The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 20: 2579 - 2583

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.20.004

Basic Research

Effect of chronic high fat diet on brain injury in ischemia/reperfusion rats and its mechanisms

  • Jiashan LI ,
  • Debin YANG ,
  • Zhifeng. PENG
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  • 1.Department of Anatomy，School of Medicine，Shanxi Datong University，Datong 037009，China

Received date: 2023-06-17

  Online published: 2023-11-15

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Abstract

Objective To evaluate the effect of chronic high fat diet （HFD） on brain injury in ischemia/reperfusion （I/R） rats and its mechanisms. Methods Sixty male Wistar rats were randomly divided into normal diet （ND） group （n = 30） and high fat diet （HFD） group （n = 30）. Each group was further divided into Sham group and I/R group， with 15 rats in each group. Neurobehavioral method was used to evaluate the neurological deficits of rats in each group； HE staining was used to detect the pathological changes of brain tissue of rats in each group； the survival rate of neurons in each group was measured by Nissl staining； western blotting method was used to detect the Pyroptosis-related proteins （NLRP3， Caspase-1， IL-1β， and HMGB1/TLR4/NF-κB signal pathway protein expression in brain tissue of rats in each group. Results Compared with the ND group， the body weight， visceral adipose tissue， triglyceride， total cholesterol and glucose water of rats in HFD group were increased （all P < 0.05）. In the I/R group， compared with the ND rats， HFD rats showed more severe neurological impairment； HE staining showed that HFD rats had more damaged cells； niss staining showed that the number of surviving neurons in HFD rats decreased； the expressions of pyroptosis related proteins （NLRP3， Caspase-1， IL-1β） were higher in HFD rats； high expression of HMGB1 and its downstream factors TLR4 and NF-κB in HFD rats （all P < 0.05）. Conclusion HFD intake could aggravate pyrodeath and enhance the expression of HMGB1/TLR4/NF-κB signaling pathway after brain I/R injury， leading to adverse outcomes after I/R.

Key words： high fat diet; ischemia/reperfusion; pyroptosis; rats

Cite this article

Jiashan LI , Debin YANG , Zhifeng. PENG . Effect of chronic high fat diet on brain injury in ischemia/reperfusion rats and its mechanisms[J]. The Journal of Practical Medicine, 2023 , 39(20) : 2579 -2583 . DOI: 10.3969/j.issn.1006-5725.2023.20.004

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