Abstract:

Objective To explore the changes of intestinal flora during Mycobacterium tuberculosis infec⁃ tion and conventional anti⁃tuberculosis chemotherapy，and to provide scientific basis for clinical treatment of tuber⁃ culosis. Methods Genomic DNA was extracted from 89 stool samples，and the changes of intestinal microflora during mycobacterium tuberculosis infection and conventional anti ⁃ tuberculosis chemotherapy were analyzed by metagenomic sequencing and bioinformatics methods. Results After mycobacterium tuberculosis infection，the composition of intestinal microflora in tuberculosis patients was significantly changed（P = 0.0134）. With the pro⁃ longation of conventional anti⁃tuberculosis chemotherapy，the Shannon index decreased，and the diversity of intesti⁃ nal flora in tuberculosis patients was significantly reduced（P = 0.0088），and the composition of bacterial flora in tuberculosis patients after cure was significantly changed compared with that before treatment（P = 0.0021）. The relative abundances of Bacteroides and Veillonella increased gradually during Mycobacterium tuberculosis infection and routine anti⁃tuberculosis chemotherapy. Some functional pathways in the gut microbiota of tuberculosis patients were changed after anti⁃tuberculosis chemotherapy. Conclusion Mycobacterium tuberculosis infection and conven⁃ tional anti⁃tuberculosis chemotherapy can lead to disturbance of intestinal flora，affecting the diversity，structural composition and metabolic function of intestinal flora.

Key words: Mycobacterium tuberculosis； , intestinal flora； , anti?tuberculosis drugs