Abstract:

Objective To investigate the effects of Interleukin⁃17A on rat bronchial fibroblasts prolifera⁃ tion，autophagy and collagen degradation after autophagy. Methods Enzyme combined digestion method and tissue block adherence method was used to culture and identify BF. CCK⁃8 method was used to detect cell prolifera⁃ tion. Experimental groups：control group，rapamycin group，IL ⁃17A + rapamycin group，IL⁃17A group. The forma⁃ tion of autophagic vesicles was observed by transmission electron microscopy. The expression of autophagy marker proteins LC3 and p62 in BF were detected by Western blot. ELISA was used to detect matrix metalloproteinase⁃ 1 and matrix metalloproteinase inhibitor expression. Results The proliferation ability of BF cells was best when the IL⁃17A concentration was 20 ng/ml. The number of autophagic vesicles in the IL⁃17A group was significantly reduced， the ratio of LC3⁃II/LC3⁃I and the expression level of MMP⁃1 were significantly down⁃regulated，and the expression levels of p62 and TIMP⁃1 were significantly up⁃regulated（P < 0.05）. Rapamycin partially reversed the effects of IL⁃17A. Conclusion IL⁃17A can promote the proliferation of BF cells. IL⁃17A also can inhibit the degradation of collagen by inhibiting autophagy，and promote the occurrence and development of airway remodeling

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