胞磷胆碱钠联合尤瑞克林对缺血性脑卒中患者miR-17-5p、miR-29b表达的影响

doi:10.3969/j.issn.1006-5725.2024.19.012

Abstract

Abstract:

Objective To investigate the effects of citicoline sodium and Ureicrin on the expression of miR-17-5p and miR-29b in patients with ischemic stroke. Methods A total of 100 patients with ischemic stroke treated in the hospital were divided into control group and combination group by random table method. From February 2023 to February 2024， they were respectively given Eurecrine monotherapy and citicoline sodium and eurecrine combination therapy. Cerebral blood perfusion indexes， inflammatory factors， oxidative stress， miR-17-5p， miR-29b levels and clinical efficacy were detected in the two groups， and adverse reactions were statistically recorded. Results There was no significant difference in CBF， CBV， hs-CRP， miR-29b， IL-6， TNF-α and miR-17-5p between the two groups before treatment （P > 0.05）. After treatment， the levels of CBF， CBV， miR-29b， SOD and GSH-Px increased， while the levels of hs-CRP， IL-6， TNF-α， miR-17-5p and MDA decreased， and the levels of CBF， CBV， miR-29b， SOD and GSH-Px in the combined group were higher than those in the control group. The levels of hs-CRP， TNF-α， IL-6， miR-17-5p and MDA were significantly different from those of the control group （P < 0.05）. The incidence of adverse reactions and the total effective rate of treatment in combination group were higher than those in control group （P < 0.05）， and the adverse reactions were mild and tolerable. Conclusion The combination of citicoline sodium and Ureicrin can improve the clinical efficacy and prognosis of patients.

Key words: citicoline sodium, eurekline, ischemic stroke, miR-17-5p, miR-29b

CLC Number:

R743.3

Baozhu ZHAO,Zhengming DU,Xiuxiu. CHEN. Effect of citicoline sodium combined with Eurekline on the expression of miR⁃17⁃5p and miR⁃29b in patients with ischemic stroke[J]. The Journal of Practical Medicine, 2024, 40(19): 2733-2737.

Figures/Tables 7

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References 25

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基因		序列
miR-17-5p	上游	5′-CCAGGATCCTTTATAGTTGTTAGAGTTTG-3′
miR-17-5p	下游	5′-CGGAATTCTAATCTACTTCACTATCTGCAC-3′
miR-29b	上游	5′-GGGTAGCACCATTTGAAATC-3′
miR-29b	下游	5′-TTTGGCACTAGCACATT-3′
U6内参	上游	5′-CTCGCTTCGGCAGCACA-3′
U6内参	下游	5′-AACGCTTCACGAATTTGCGT-3′

组别	例数	CBF/［mL/（100g·min）］		CBV/（mL/100g）
组别	例数	治疗前	治疗后	治疗前	治疗后
对照组	50	17.60 ± 1.39	20.72 ± 2.10	1.72 ± 0.28	2.02 ± 0.18
联合组	50	17.63 ± 1.35	23.85 ± 2.76	1.74 ± 0.23	2.35 ± 0.15
t值		0.110	6.382	0.390	9.959
P值		0.913	< 0.001	0.697	< 0.001

组别	例数	hs-CRP/（mg/L）		IL-6/（ng/mL）		TNF-α/（pg/mL）
组别	例数	治疗前	治疗后	治疗前	治疗后	治疗前	治疗后
对照组	50	17.44 ± 6.24	10.17 ± 3.55	16.14 ± 2.47	12.24 ± 2.36	46.24 ± 5.47	25.94 ± 4.03
联合组	50	17.48 ± 6.52	5.51 ± 1.13	16.18 ± 2.51	9.34 ± 1.03	46.28 ± 5.41	14.34 ± 2.16
t值		0.003	8.845	0.080	7.964	0.037	1.937
P值		0.975	< 0.001	0.936	< 0.001	0.971	< 0.001

组别	例数	miR-17-5p		miR-29b
组别	例数	治疗前	治疗后	治疗前	治疗后
对照组	50	3.62 ± 1.23	1.12 ± 0.94	0.39 ± 0.15	0.73 ± 0.16
联合组	50	3.64 ± 1.25	0.25 ± 0.76	0.37 ± 0.14	1.95 ± 0.35
t值		0.081	5.089	0.689	22.420
P值		0.936	< 0.001	0.492	< 0.001

组别	例数	MDA/（μmol/L）		SOD/（U/mL）		GSH-Px/(U/mL)
组别	例数	治疗前	治疗后	治疗前	治疗后	治疗前	治疗后
对照组	50	14.14 ± 1.84	9.52 ± 1.13	67.44 ± 9.37	85.74 ± 11.46	47.81 ± 6.34	67.64 ± 8.23
联合组	50	14.48 ± 1.82	6.51 ± 0.84	67.46 ± 9.52	108.34 ± 15.73	47.53 ± 6.87	89.24 ± 11.16
t值		0.929	15.120	0.011	8.211	0.212	11.010
P值		0.355	< 0.001	0.992	< 0.001	0.833	< 0.001

Effect of citicoline sodium combined with Eurekline on the expression of miR⁃17⁃5p and miR⁃29b in patients with ischemic stroke

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组别	例数	痊愈	有效	无效	总有效率
对照组	50	10	15	10	41（80.39）
联合组	50	17	10	1	51（98.07）
χ²值					8.442
P值					0.004

组别	例数	腹泻	皮疹	恶心	总发生率
对照组	50	0	1	1	2（3.92）
联合组	50	3	3	3	9（17.31）
χ²值					4.863
P值					0.027

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