Abstract:

Objective To explore the effect and mechanism of AMD3100 on CXCR4 and MUC5ac in asthma mice. Methods Female BALB/c mice aged to 6 ~ 8 weeks were randomly divided into normal group（NS group）， asthma group（AS group），CXCR4 blockers group（AMD group），Eight in each group.Asthma was induced by OVA sensitization and challenge，intervention was performed in each group before stimulation. IL ⁃ 4，IL ⁃5，CXCR4 in BALF were detected by ELISA.The pathological changes of lung were observed by HE staining，AB ⁃PAS staining； IHC and RT⁃PCR were used to detect the expression of Muc5ac，CXCR4 protein level and CXCR4 mRNA in lung tissues. Results The CXCR4，IL⁃4 and IL⁃5 levels，infiltration of inflammatory cells around the airway of lung tis⁃ sue，positive relative coloring area of airway mucus，CXCR4 and Muc5ac protein content，mRNA relative expression of CXCR4，AMD group were lower than AS group（P < 0.01）. was higher than NS group（P < 0.05）. Conclusions AMD3100 intervention can reduce the expression of CXCR4 protein，inhibits airway inflammatory and down regu⁃ lates the expression of MUC5AC in asthmatic mice，alleviate airway mucus hypersecretion，and improves asthmatic symptoms.

Key words:

bronchial asthma, AMD3100, CXC chemokine receptor 4, Mucin5AC