电针心包经穴对APP/PS1小鼠脑功能网络连接的影响

doi:10.3969/j.issn.1006-5725.2026.08.001

Abstract

Abstract:

Objective To explore the effect of electroacupuncture at the Neiguan and Jianshi acupoints on the brain functional network connectivity in APP/PS1 mice. Methods A total of 18 5-month-old SPF-grade male mice were selected， among which 12 model mice carrying the APP/PS1 double transgenic were randomly divided into two groups according to the random number table method： the model group （n = 6） and the electroacupuncture group （n = 6）. Additionally， 6 C57BL/6 mice with the same genetic background were selected as the control group. The mice in the electroacupuncture group were given electroacupuncture intervention at the "Neiguan" and "Jianshi" acupoints. The electroacupuncture device was connected to the acupoints on the same side， and the acupoints on both sides were alternately used once every other day for a total of 5 weeks. After electroacupuncture， the spatial learning and memory ability of mice in each group was detected by Morris water maze， and the non-spatially dependent memory ability of mice in each group was observed by new object recognition. Taking the bilateral hippocampus as the region of interest （ROI）， resting-state functional magnetic resonance imaging （rs-fMRI） was used to analyze the functional connectivity between the hippocampus and the whole brain. The changes in the contents of norepinephrine （NE） and dopamine （DA） in the hippocampus and prefrontal cortex were detected by ELISA. The expression levels of glycogen synthase kinase 3β （GSK-3β） and synaptic protein I（SynI） in the hippocampus were detected by Western blot. Results Compared with the control group， the latency of mice in the model group was prolonged （P < 0.01）， the number of platform crossings and the exploration time of the target quadrant decreased （P < 0.01）， and the cognitive index decreased （P < 0.01）. Compared with the model group， the latency of mice in the electroacupuncture group was shortened （P < 0.01）， the number of platform crossings and the exploration time of the target quadrant were both increased （P < 0.01）， and the cognitive index was elevated （P < 0.01）. Compared with the control group， the functional connections of the transition area between the right hippocampus and the left posterior piriform region， the right central amygdala， the left dentate gyrus， the right entorhinal region， and the right main olfactory bulb in the model group mice were decreased. Compared with the model group， the functional connections of the transition area between the right hippocampus and the left posterior piriform region， the right central amygdala， the transition area of the right posterior piriform region， the left dentate gyrus， the right main olfactory bulb， and the left main olfactory bulb in the electroacupuncture group were increased. The functional connections between the left hippocampus and the right lateral nucleus， the right hippocampal subCA3， the left main olfactory bulb， and the left entorhinal area were elevated. Compared with the control group， the contents of NE and DA in the hippocampus of mice in the model group decreased （P < 0.05）， and the contents of NE and DA in the prefrontal cortex decreased （P < 0.05）. Compared with the model group， the contents of NE and DA in the hippocampus of mice in the electroacupuncture group increased （P < 0.05）， and those in the prefrontal cortex increased （P < 0.05）. Compared with the control group， the expression of GSK-3β in the hippocampus of mice in the model group increased and the expression of SynI decreased （P < 0.05）. Compared with the model group， the expression of GSK-3β in the hippocampus of mice in the electroacupuncture group decreased and the expression of SynI increased （P < 0.05）. Conclusions Electroacupuncture at the pericardium acupoint can restore the functional connectivity between the hippocampus and other brain regions in APP/PS1 mice. The mechanism may be related to the disorder of neurotransmitters regulating the key brain regions of AD.

Key words: Alzheimer's disease, electroacupuncture, pericardium meridian points, functional connectivity, neurotransmitters

CLC Number:

R245

Xun ZHANG,Juan XIAO,Chaochao YU,Zhaoxie YU,Zhipeng FENG,Yankun PAN,Shuo YANG,Feng SHEN. The effect of electroacupuncture at the Heart-Pericardium Meridian points on the brain functional network connectivity in APP/PS1 mice[J]. The Journal of Practical Medicine, 2026, 42(8): 1301-1311.

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References 45

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组别	第1天	第2天	第3天	第4天	第5天
对照组	55.90 ± 4.12	38.83 ± 4.84	29.89 ± 3.39	18.79 ± 4.26	6.62 ± 2.78
模型组	58.22 ± 2.71	52.70 ± 5.61^*	46.88 ± 4.23^*	39.64 ± 3.74^*	28.41 ± 4.26^*
电针组	57.77 ± 3.88	39.75 ± 5.22^#	31.11 ± 2.64^#	19.65 ± 4.59^#	6.97 ± 3.14^#
F值	0.836	7.972	44.375	30.566	41.336
P值	0.455	< 0.01	< 0.01	< 0.01	< 0.01

组别	穿越平台次数/次	目标象限探索时间/s
对照组	8.00 ± 1.41	36.25 ± 5.17
模型组	2.00 ± 0.89^*	10.62 ± 3.08^*
电针组	6.50 ± 2.07^#	35.14 ± 7.20^#
F值	24.718	42.884
P值	< 0.01	< 0.01

组别	认知指数
对照组	68.58 ± 9.38
模型组	33.39 ± 4.74^*
电针组	66.59 ± 9.38^#
F值	35.43
P值	< 0.01

脑区	T值	体素	MNI峰值坐标
脑区	T值	体素	X	Y	Z
右侧后梨状区过渡区域	-4.230 9	22	33	-18	-21
左侧主嗅球	-4.456 0	43	-3	30	9
左侧内嗅区	-3.256 9	12	-27	-33	9

脑区	T值	体素	MNI峰值坐标
脑区	T值	体素	X	Y	Z
右侧外侧隔核	3.784 6	29	6	12	-6
右侧海马亚CA3	3.752 3	33	21	-18	-12
左侧主嗅球	3.575 5	9	-6	45	6
左侧内嗅区	3.081 6	9	-27	-33	6

The effect of electroacupuncture at the Heart-Pericardium Meridian points on the brain functional network connectivity in APP/PS1 mice

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组别	DA含量/（ng/mL）	NE含量/（pg/mL）
对照组	145.17 ± 2.66	1 305.08 ± 60.47
模型组	91.63 ± 5.35^*	1 036.91 ± 27.10^*
电针组	136.20 ± 21.00^#	1 263.94 ± 96.63^#
F值	10.35	9.117
P值	< 0.05	< 0.05

组别	DA含量/（ng/mL）	NE含量/（pg/mL）
对照组	170.89 ± 6.62	961.09 ± 58.40
模型组	131.65 ± 6.16^*	794.31 ± 20.23^*
电针组	160.96 ± 12.41^#	906.94 ± 38.50^#
F值	15.877	12.288
P值	< 0.01	< 0.01

组别	GSK-3β/β-actin	SynI/β-actin
对照组	0.03 ± 0.01	0.44 ± 0.04
模型组	0.66 ± 0.05^*	0.23 ± 0.02^*
电针组	0.19 ± 0.04^#	0.38 ± 0.02^#
F值	224.135	45.794
P值	< 0.01	< 0.01

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[8]	LI Hanzhang, QI Ling, ZHANG Xiaolei, CHEN Xianglin, GUO Lei, GUO Shuqin, MA Jun.. Effect of electroacupuncture on GLP⁃1R⁃mediated PI3K/AKT/GSK⁃3β pathway in mice with Parkinson′s disease [J]. The Journal of Practical Medicine, 2021, 37(21): 2712-2716.

脑区	T值	体素	MNI峰值坐标
脑区	T值	体素	X	Y	Z
左侧后梨状区过渡区域	-3.766 5	13	-33	-18	-15
右侧中央杏仁核	-6.223 3	30	24	-6	-15
左侧齿状回	-5.342 5	17	-18	-24	-12
右侧内嗅区	-4.019 0	59	45	-21	-3
右侧主嗅球	-3.044 1	11	9	42	12