强直性脊柱炎患者环状RNA hsa_circ_0001707的表达及意义

doi:10.3969/j.issn.1006-5725.2026.02.015

Abstract

Abstract:

Objective To investigate the clinical value of circular RNAs （circRNAs） in the diagnosis and disease activity assessment of ankylosing spondylitis （AS） by measuring the expression levels of hsa_circ_0001707 and hsa_circ_0075522 in peripheral blood mononuclear cells （PBMCs） of AS patients. Methods A case?control study was conducted， enrolling 88 newly diagnosed AS patients and 80 healthy controls （HCs） with matched baseline characteristics. The expression of hsa_circ_0001707， hsa_circ_0075522， and HLA?B27 was detected via quantitative polymerase chain reaction （qPCR）. Disease activity was evaluated using the Bath Ankylosing Spondylitis Disease Activity Index （BASDAI）. Erythrocyte sedimentation rate （ESR）， C?reactive protein （CRP）， and complete blood count parameters were measured using automated analyzers. Novel inflammatory indices， including the lymphocyte?to?monocyte ratio （LMR）， neutrophil?to?lymphocyte ratio （NLR）， platelet?to?lymphocyte ratio （PLR）， derived NLR （dNLR）， and systemic immune?inflammation index （SII）， were calculated. Results circRNA analysis revealed significantly upregulated hsa_circ_0001707 and downregulated hsa_circ_0075522 in AS patients （both P < 0.05）. Receiver operating characteristic （ROC） curve analysis demonstrated that hsa_circ_0001707 exhibited moderate diagnostic efficacy for distinguishing AS from HCs （AUC = 0.677， P < 0.001）， which significantly improved when combined with dNLR （AUC = 0.835， P < 0.001）. In disease activity assessment， hsa_circ_0001707 levels were significantly higher in active AS （BASDAI ≥ 4） than in inactive patients and correlated with CRP. Its discriminative power for active AS surpassed that of CRP （AUC： 0.684 vs. 0.674）. Correlation analysis further supported the potential role of hsa_circ_0001707 in AS pathogenesis， showing positive associations with inflammatory markers （NLR， SII， CRP） and BASDAI （ P < 0.05）， but negative correlations with lymphocyte percentage （L%） and LMR. In contrast， hsa_circ_0075522 showed no significant clinical correlations. Conclusions Hsa_circ_0001707 may serve as a promising peripheral blood biomarker for AS diagnosis and disease activity monitoring.

Key words: ankylosing spondylitis, circRNA hsa_circ_0001707, biomarker, disease activity, inflammation, diagnostic model

CLC Number:

R593.23

Yao ZHOU,Qianbin DAI,Wei LONG,Kaixiang HU,Rui WU,Biqi FU. Expression and significance of circular RNA hsa_circ_0001707 in ankylosing spondylitis[J]. The Journal of Practical Medicine, 2026, 42(2): 295-302.

Figures/Tables 6

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Tab.2

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References 27

[1]	MAURO D， THOMAS R， GUGGINO G， et al. Ankylosing spondylitis： An autoimmune or autoinflammatory disease？［J］. Nat Rev Rheumatol， 2021， 17（7）： 387-404. doi：10.1038/s41584-021-00625-y . doi: 10.1038/s41584-021-00625-y
[2]	黄烽，朱剑，王玉华，等. 强直性脊柱炎诊疗规范［J］. 中华内科杂志， 2022， 61（8）： 893-900. doi：10.3760/cma.j.cn112138-20211226-00913 . doi: 10.3760/cma.j.cn112138-20211226-00913
[3]	EBRAHIMIADIB N， BERIJANI S， GHAHARI M， et al. Ankylosing spondylitis［J］. J Ophthalmic Vis Res， 2021， 16（3）： 462-469. doi：10.18502/jovr.v16i3.9440 . doi: 10.18502/jovr.v16i3.9440
[4]	NGO L H， BERT A G， DREDGE B K， et al. Nuclear export of circular RNA［J］. Nature， 2024， 627（8002）： 212-220. doi：10.1038/s41586-024-07060-5 . doi: 10.1038/s41586-024-07060-5
[5]	NIU D， WU Y， LIAN J. Circular RNA vaccine in disease prevention and treatment［J］. Signal Transduct Target Ther， 2023， 8（1）： 341. doi：10.1038/s41392-023-01561-x . doi: 10.1038/s41392-023-01561-x
[6]	LUO Q， YE Y， ZHANG L， et al. Hsa_circ_0044235 and hsa_circ_0001947 as novel biomarkers in plasma of patients with new-onset systemic lupus erythematosus［J］. J Immunotoxicol， 2023， 20（1）： 2196453. doi：10.1080/1547691X.2023.2196453 . doi: 10.1080/1547691X.2023.2196453
[7]	LUO Q， ZENG L， ZENG L， et al. Expression and clinical significance of circular RNAs hsa_circ_0000175 and hsa_circ_0008410 in peripheral blood mononuclear cells from patients with rheumatoid arthritis［J］. Int J Mol Med， 2020， 45（4）： 1203-1212. doi：10.3892/ijmm.2020.4498 . doi: 10.3892/ijmm.2020.4498
[8]	PAN H， WU S， WANG J， et al. TNFRSF₂₁ mutations cause high myopia［J］. J Med Genet， 2019， 56（10）： 671-677. doi：10.1136/jmedgenet-2018-105684 . doi: 10.1136/jmedgenet-2018-105684
[9]	MA J， HUANG L， GAO Y B， et al. M2 macrophage facilitated angiogenesis in cutaneous squamous cell carcinoma via circ_TNFRSF₂1/miR-3619-5p/ROCK axis［J］. Kaohsiung J Med Sci， 2022， 38（8）： 761-771. doi：10.1002/kjm2.12555 . doi: 10.1002/kjm2.12555
[10]	CUI H， LIU Y， ZHENG Y， et al. Intelectin enhances the phagocytosis of macrophages via CDC42-WASF₂-ARPC2 signaling axis in Megalobrama amblycephala ［J］. Int J Biol Macromol， 2023， 236： 124027. doi：10.1016/j.ijbiomac.2023.124027 . doi: 10.1016/j.ijbiomac.2023.124027
[11]	SUN L N， XING C， ZHI Z， et al. Dicer suppresses cytoskeleton remodeling and tumorigenesis of colorectal epithelium by miR-324-5p mediated suppression of HMGXB3 and WASF-2［J］. Oncotarget， 2017， 8（34）： 55776-55789. doi：10.18632/oncotarget.18218 . doi: 10.18632/oncotarget.18218
[12]	LUO Q， ZHANG L， FANG L， et al. Circular RNAs hsa_circ_0000479 in peripheral blood mononuclear cells as novel biomarkers for systemic lupus erythematosus［J］. Autoimmunity， 2020， 53（3）： 167-176. doi：10.1080/08916934.2020.1728529 . doi: 10.1080/08916934.2020.1728529
[13]	LIVAK K J， SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 （-Delta Delta C（T）） Method［J］. Methods， 2001， 25（4）： 402-408. doi：10.1006/meth.2001.1262 . doi: 10.1006/meth.2001.1262
[14]	冯其柱，卢曼曼，孙杰，等. 新型全身性炎症指标对急性胰腺炎早期病情严重程度的预测价值［J］. 实用医学杂志， 2024， 40（14）： 1963-1968. doi：10.3969/j.issn.1006-5725.2024.14.011 . doi: 10.3969/j.issn.1006-5725.2024.14.011
[15]	刘剑，李敏菁. 全身免疫炎症指数对非小细胞肺癌免疫检查点抑制剂疗效的预测价值［J］. 实用医学杂志， 2022， 38（7）： 904-908. doi：10.3969/j.issn.1006-5725.2022.07.024 . doi: 10.3969/j.issn.1006-5725.2022.07.024
[16]	RAPAPA K A， DENG Y， PENG Y， et al. The association and clinical significance of hematological biomarkers in ankylosing spondylitis［J］. Sci Rep， 2025， 15（1）： 15755. doi：10.1038/s41598-025-99274-4 . doi: 10.1038/s41598-025-99274-4
[17]	SARIYILDIZ A， BENLIDAYI I C， TURK I， et al. Evaluation of the relationship between blood cell markers and inflammation， disease activity， and general health status in ankylosing spondylitis［J］. Rev Assoc Med Bras， 2023， 69（10）： e20230722. doi：10.1590/1806-9282.20230722 . doi: 10.1590/1806-9282.20230722
[18]	MANGONI A A， ZINELLU A. The diagnostic role of the systemic inflammation index in patients with immunological diseases： A systematic review and meta-analysis［J］. Clin Exp Med， 2024， 24（1）： 27. doi：10.1007/s10238-024-01294-3 . doi: 10.1007/s10238-024-01294-3
[19]	BOYRAZ İ， KOÇ B， ÇOĞALGIL S. Assessment of neutrophil- lymphocyte and platelet- lymphocyte ratios in ankylosing spondylitis［J］. Arch Rheumatol， 2016， 31（2）： 199-200. doi：10.5606/ArchRheumatol.2016.5729 . doi: 10.5606/ArchRheumatol.2016.5729
[20]	COŞKUN B N， ÖKSÜZ M F， ERMURAT S， et al. Neutrophil lymphocyte ratio can be a valuable marker in defining disease activity in patients who have started anti-tumor necrosis factor （TNF） drugs for ankylosing spondylitis［J］. Eur J Rheumatol， 2014， 1（3）： 101-105. doi：10.5152/eurjrheumatol.2014.034 . doi: 10.5152/eurjrheumatol.2014.034
[21]	BRAUN J， SIEPER J. Fifty years after the discovery of the association of HLA B27 with ankylosing spondylitis［J］. RMD Open， 2023， 9（3）： e003102. doi：10.1136/rmdopen-2023-003102 . doi: 10.1136/rmdopen-2023-003102
[22]	余明阳，李甲，冯新哲，等. 强直性脊柱炎中Th17细胞分化调控机制及其治疗靶点的研究进展［J］. 实用医学杂志， 2025， 41（18）： 2953-2960. doi：10.3969/j.issn.1006-5725.2025.18.024 . doi: 10.3969/j.issn.1006-5725.2025.18.024
[23]	LUO Q， WU Z， XIAO Q， et al. The significance of m6A-circFOXK2 in CD4⁺T cells from patients with rheumatoid arthritis and circFOXK2 correlates with Th17% and autophagy［J］. J Transl Autoimmun， 2025， 11： 100326. doi：10.1016/j.jtauto. 2025.100326 . doi: 10.1016/j.jtauto. 2025.100326
[24]	RAMIRO S， VAN DER HEIJDE D， VAN TUBERGEN A， et al. Higher disease activity leads to more structural damage in the spine in ankylosing spondylitis： 12-year longitudinal data from the OASIS cohort［J］. Ann Rheum Dis， 2014， 73（8）： 1455-1461. doi：10.1136/annrheumdis-2014-205178 . doi: 10.1136/annrheumdis-2014-205178
[25]	PINA VEGAS L， SBIDIAN E， WENDLING D， et al. Factors associated with remission at 5-year follow-up in recent-onset axial spondyloarthritis： Results from the DESIR cohort［J］. Rheumatology， 2022， 61（4）： 1487-1495. doi：10.1093/rheumatology/keab565 . doi: 10.1093/rheumatology/keab565
[26]	SU J， CUI L， YANG W， et al. Baseline high-sensitivity C-reactive protein predicts the risk of incident ankylosing spondylitis： Results of a community-based prospective study［J］. PLoS One， 2019， 14（2）： e0211946. doi：10.1371/journal.pone. 0211946 . doi: 10.1371/journal.pone. 0211946
[27]	BARALIAKOS X， SZUMSKI A， KOENIG A S， et al. The role of C-reactive protein as a predictor of treatment response in patients with ankylosing spondylitis［J］. Semin Arthritis Rheum， 2019， 48（6）： 997-1004. doi：10.1016/j.semarthrit.2018.10.019 . doi: 10.1016/j.semarthrit.2018.10.019

目的基因	前引物（F）	后引物（R）
hsa_circ_0001707	ATTGAGCTCCCCACAGAAAC	TTAGTAATTCCCCAACGTGCCA
hsa_circ_0075522	TTGGCAGCGTCTTCTGTTGT	TGGTTGTCCCATTGGCTGTT
GADPH	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAG

项目	AS组（n = 88）	健康对照组（n = 80）	t/χ2/Z值	P值
性别/例			0.074	0.785
男	61	57
女	27	23
年龄/岁	32.15 ± 10.66	32.98 ± 9.38	-0.593	0.593
病程/年	4.56 ± 6.13
BASDAI/分	3.99 ± 1.33
HLA-27阳性/［例（%）］	78（88.64）
ESR/（mm/h）	20.86 ± 21.43
CRP/（mg/L）	16.33 ± 26.54
WBC/（×10⁹·L^-1）	7.54 ± 2.50	6.38 ± 1.22	3.855	< 0.001
RBC/（×10¹²·L^-1）	4.84 ± 0.65	5.03 ± 0.44	-2.16	0.032
HGB/（g/L）	138.61 ± 17.06	149.9 ± 20.91	-3.845	< 0.001
HCT/（L/L）	0.43 ± 0.05	0.45 ± 0.04	-4.197	< 0.001
PLT/（×10⁹·L^-1）	288.1 ± 83.37	239.21 ± 39.97	4.915	< 0.001
MPV［M（P₂₅，P₇₅）］/fl	9.50（9.00，10.4）	10.45（10.00，11.10）	-5.62	< 0.001
PCT/%	0.28 ± 0.07	0.25 ± 0.04	-2.958	0.004
PDW/fl	14.66 ± 2.38	12.7 ± 1.68	6.176	< 0.001
L/（×10⁹·L^-1）	3.88 ± 18.57	2.17 ± 0.46	0.822	0.412
L/%	26.32 ± 6.26	34.53 ± 6.99	-8.027	< 0.001
M/（×10⁹·L^-1）	0.44 ± 0.14	0.45 ± 0.14	-0.451	0.653
M/%	6.00 ± 1.55	7.05 ± 1.65	-4.263	< 0.001
N/（×10⁹·L^-1）	5.02 ± 2.25	3.60 ± 0.97	5.379	< 0.001
N/%	65.46 ± 7.7	55.89 ± 7.18	8.301	< 0.001
LMR	10.77 ± 58.16	5.18 ± 1.62	5.632	< 0.001
PLR	116.26 ± 66.03	115.58 ± 35.47	0.858	0.392
NLR	2.79 ± 1.49	1.76 ± 0.87	5.464	< 0.001
DNLR	2.38 ± 0.96	1.34 ± 0.46	6.491	< 0.001
SII	844.14 ± 734.91	417.44 ± 199.6	5.025	< 0.001
ECR	6.58 ± 15.44
hsa_circ_0001707［M（P₂₅，P₇₅）］	3.04（0.88，15.5）	1.00（0.3，2.51）	3.959	< 0.001
hsa_circ_0075522［M（P₂₅，P₇₅）］	0.77（0.4，1.41）	1.75（0.43，2.87）	3.190	0.001

项目	HLA?B27阴性（n = 10）	HLA?B27阳性（n = 78）	Z值	P值
hsa_circ_0001707	17.79（1.90，40.93）	2.47（0.84，8.40）	-1.51	0.133
hsa_circ_0075522	1.14（0.33，1.76）	0.73（0.40，6.04）	-0.72	0.479

Expression and significance of circular RNA hsa_circ_0001707 in ankylosing spondylitis

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