微小RNA-378-5p对胶质瘤血管生成的影响

doi:10.3969/j.issn.1006-5725.2025.02.005

Abstract

Abstract:

Objective To investigate the effect of miR-378-5p on glioma angiogenesis and its molecular mechanism. Methods The expression of miR-378-5p were detected by qRT-PCR in four glioma cells. The levels of VEGF were analyzed by ELISA. HUVECs were treated conditioned medium which collected by glioma cells supernatant. The effects of miR-378-5p on the proliferation， migration， invasion， and angiogenesis of HUVECs were detected by CCK-8， Transwell assay， and tubular formation assay. Results U251 was the highest expression of miR-378-5p， while U87 MG was the lowest expression. Glioma-conditioned media increased miR-378-5p levels significantly promoted the proliferation， migration， invasion， and angiogenesis of HUVECs. The results were contrary with glioma-conditioned media decreased miR-378-5p levels. miR-378-5p promoted the release of VEGF-A from glioma cells. The proliferation， migration， invasion， and angiogenesis of HUVECs treated-VEGF neutralizing antibody were significantly downregulated. Conclusion miR-378-5p promoted HUVECs proliferation， migration， invasion， and angiogenesis by increasing the release of VEGF from glioma cells.

Key words: microRNA-378-5p, glioma, angiogenesis, VEGF-A

CLC Number:

R739.41

Jiayi CAI,Siyu CHEN,Nyulue CAI,Wende. LI. Effect of miR⁃378⁃5p on angiogenesis of glioma[J]. The Journal of Practical Medicine, 2025, 41(2): 186-194.

Figures/Tables 9

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引物名称	引物序列（5′-3′）
miR-378-5p-RT	GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACACA
miRNA通用引物	CTCCTGACTCCAGGTCC
U6-RT	GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAAAATATG
U6-FT	TTAGCATGGCCCCTGCGC

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Effect of miR⁃378⁃5p on angiogenesis of glioma

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