阿加曲班联合线粒体移植对脑缺血再灌注损伤小鼠内皮功能及血流变学的影响

doi:10.3969/j.issn.1006-5725.2024.19.001

Abstract

Abstract:

Objective To analyze the effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia?reperfusion injury. Methods 50 SPF grade Kunming mice， with 10 as the normal group，38 mices were successfully modeled and divided into a model group of 9， a mitochondrial transplantation group of 9， an Agatroban group of 10， and a combination group of 10. The mitochondrial transplantation group was given exogenous mitochondrial extract， the agatroban group was given agatroban， the combined group was given agatroban combined with mitochondrial transplantation， and the blank group and model group were injected with normal saline. Observation of changes in cerebral infarction volume， pathological morphology of brain tissue， endothelial function， hemorheology， and relative expression of apoptosis related proteins in mice after 7 days of intervention. Results Compared with normal group， cerebral infarction volume ratio， ET?1， whole blood viscosity， plasma viscosity， Caspase-3 and Bax protein expression levels were increased in model group， mitochondrial transplantation group， agatroban group and combined group， while NO level and Bcl-2 protein expression levels were decreased （P < 0.05）. Compared with the model group， the cerebral infarction volume ratio， ET-1， whole blood viscosity， plasma viscosity， Caspase-3 and Bax protein expressions in the mitochondrial transplantation group， agatroban group and combined group were decreased， and the combined group was lower than the mitochondrial transplantation group and agatroban group， and the NO level and Bcl-2 protein expression were increased. The combined group was higher than that of mitochondrial transplantation group and agatriban group （P < 0.05）. Conclusion In mice with cerebral ischemia-reperfusion injury， endothelial function injury was improved， hemorheology was regulated， and brain injury was alleviated after agatroban combined with mitochondrial transplantation.

Key words: agatroban, mitochondrial transplantation, cerebral ischemia-reperfusion injury, endothelial function, hemorheology

CLC Number:

R745.1

Yingjun TAO,Tengzhu REN,Feng WEI,Xintong. LIU. Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia⁃reperfusion injury[J]. The Journal of Practical Medicine, 2024, 40(19): 2665-2671.

Figures/Tables 6

Tab.1

Fig.1

Tab.2

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Tab.4

Fig.2

References 26

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组别	例数	脑梗死体积比/%
正常组	10	0.00
模型组	9	40.39 ± 5.01^a
线粒体移植组	9	31.37 ± 3.25^ab
阿加曲班组	10	31.45 ± 3.31^ab
联合组	10	23.01 ± 3.01^abcd
t值^*		0.082
P值^*		0.935
F值		9.404
P值		<0.001

组别	例数	NO（μmol/L）	ET-1（ng/L）
正常组	10	20.01 ± 3.58	132.89 ± 16.01
模型组	9	7.03 ± 0.98^a	250.36 ± 28.75^a
线粒体移植组	9	13.02 ± 1.25^ab	201.58 ± 20.01^ab
阿加曲班组	10	12.99 ± 1.26^ab	200.96 ± 21.12^ab
联合组	10	16.27 ± 2.01^abcd	160.52 ± 19.35^abcd
t值^*		0.057	0.155
P值^*		0.952	0.877
F值		2.881	3.479
P值		< 0.001	< 0.001

组别	例数	全血黏度/mPa.s			血浆黏度/mPa.s
组别	例数	低切	中切	高切	血浆黏度/mPa.s
正常组	10	7.25 ± 0.72	5.12 ± 0.58	4.03 ± 0.45	1.09 ± 0.12
模型组	9	13.98 ± 1.25^?	7.25 ± 0.98^?	6.01 ± 0.62^?	2.20 ± 0.23^?
线粒体移植组	9	11.22 ± 1.02^?#	6.52 ± 0.73^?#	5.12 ± 0.58^?#	1.78 ± 0.18^?#
阿加曲班组	10	11.30 ± 1.01^?#	6.51 ± 0.71^?#	5.11 ± 0.57^?#	1.79 ± 0.17^?#
联合组	10	9.21 ± 0.80^?#△▲	5.98 ± 0.62^?#△▲	4.52 ± 0.47^?#△▲	1.42 ± 0.15^?#△▲
t值^*		0.153	0.162	0.256	0.070
P值^*		0.876	0.871	0.797	0.942
F值		5.759	3.203	2.381	5.433
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	例数	Caspase-3	Bax	Bcl-2
正常组	10	1.02 ± 0.01	0.98 ± 0.01	0.95 ± 0.02
模型组	9	2.01 ± 0.25^?	2.23 ± 0.25^?	0.21 ± 0.03^?
线粒体移植组	9	1.69 ± 0.17^?#	1.78 ± 0.19^?#	0.45 ± 0.05^?#
阿加曲班组	10	1.70 ± 0.16^?#	1.77 ± 0.18^?#	0.44 ± 0.06^?#
联合组	10	1.25 ± 0.15^?#△▲	1.23 ± 0.14^?#△▲	0.71 ± 0.08^?#△▲
t值^*		0.312	0.220	0.415
P值^*		0.756	0.827	0.678
F值		4.838	5.633	9.204
P值		< 0.001	< 0.001	< 0.001

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Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia⁃reperfusion injury

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