Abstract:

Objective To investigate the expression of lncRNA HOXA⁃AS2 in pancreatic cancer tissues， and the effect of silencing this gene on the proliferation and invasiveness of pancreatic cancer cells. Methods A total of 97 cases of patients with pancreatic cancer underwent radical surgery in our hospital were selected from August 2015 to August 2020. The human pancreatic cancer PANC⁃1 and AsPC⁃1 cells were cultured and were divide into si⁃HOXA⁃AS2 group，si⁃Con group and blank group，respectively，transfected with the interference sequence，negative control sequence and no treatment. RT⁃PCR was used to detect the expressions of HOXA⁃AS2 in pancreatic cancer and adjacent tissues，as well as the expression of HOXA⁃AS2 of cells in different groups. CCK⁃8 and Transwell methods were used to detect cell proliferation activity and cell invasiveness. Results The expression level of HOXA⁃AS2 in pancreatic cancer tissue was（2.21 ± 0.20），which was higher than（1.03 ± 0.14）in adja⁃ cent tissues，and the difference was statistically significant（t = 48.030，P < 0.001）. The differences of the expres⁃ sion levels of HOXA⁃AS2 in pancreatic cancer tissues between different TNM stages，degree of differentiation and whether lymph node metastasis were statistically significant（P < 0.05）. In PANC⁃1 and AsPC⁃1 cells，compared with the si⁃Con group and the blank group，the expression level of HOXA⁃AS2 in the si⁃HOXA⁃AS2 group was sig⁃ nificantly decreased（P < 0.05），and the absorbance OD values at 24，48，72 and 96 h in the si ⁃HOXA ⁃AS2 group were lower than those in the si⁃Con group and the blank group（P < 0.05），and the number of invasive cells in the si⁃HOXA⁃AS2 group was lower than that in the si⁃Con group and the blank group（P < 0.05）. Conclusion The expression level of HOXA⁃AS2 is increased in pancreatic cancer tissues and in correspondance to clinical indi⁃ cators of malignant progression. Silencing its expression can reduce cell proliferation and inhibit cell invasion.

Key words:

 , pancreatic cancer, lncRNA HOXA ?AS2, clinical indicators, cell proliferation, cell invasion