Abstract:

Objective To explore the effect of Cyclin L1（CCNL1）on the biological behaviors and regula⁃ tory mechanism of human trophoblast cells. Methods We used HTR ⁃ 8/SVneo as the model and divide it into CCNL1 overexpression group，CCCNL1 overexpression control group，CCNL1 interference group 1，CCNL1 inter⁃ ference group 2，CCNL1 interference group 3 and interference control group. The mRNA expression level of CCNL1 was detected by Quantitative Real⁃time PCR（qPCR）；the protein expression level of CCNL1 by Western Blot and cell proliferation by CCK⁃8. Cell migration and invasion were detected by Transwell assay and cell cycle and cell apoptosis by flow cytometry. Results Overexpression CCNL1 significantly decreased the migration and invasion ability of trophoblast cells（P < 0.05），the proliferation ability of trophoblast cells（P < 0.05），and the number of S cells（P < 0.05）. Interfered CCNL1 significantly increased the migration and invasion ability of tropho⁃ blast cells（P < 0.05），and the proliferation ability of trophoblast cells（P < 0.05）. Conclusion CCNL1 gene may affect placental function by regulating the migration，invasion and proliferation of trophoblast cells.

Key words:

cyclin L1, trophoblast cells, fetal growth restriction, cell proliferation, cell migration