Abstract:

Objective To compare the diagnostic performances of high performance liquid chromatogra⁃ phy and capillary zone electrophoresis in the determination of HbA2 in α ⁃thalassemia. Methods The patients underwent α ⁃thalassemia screening in the Guangdong Second Provincial General Hospital from January 2019 to December 2021 were engaged in this study for the gathering of their clinical data such as HbA2 and thalassemia gene test results. The patients were divided into control group and α⁃thalassemia group according to the results of thalassemia gene detection. The indexes of the two groups were analyzed. The ROC was used to evaluate and com⁃ pare the diagnostic performances and cut⁃off point of HbA2 in α⁃thalassemia detected by high performance liquid chromatography and capillary zone electrophoresis. Results HbA2 in the α⁃thalassemia group was both significantly decreased when detected by both high performance liquid chromatography，as compared to the control group ［（2.78 ± 0.39）% vs.（2.57 ± 0.21）%，P < 0.001）］or capillary zone electrophoresis［（2.62 ± 0.46）% vs.（2.34 ± 0.31）%，P < 0.001）］. When detected by high performance liquid chromatography，the AUC of the HbA2 of ROC was 0.715（95%CI：0.682 ~ 0.747，P < 0.001）and the cut⁃off point was 2.8%，with sensitivity and specificity of 82.6% and 55.7%，respectively，while detected by capillary zone electrophoresis，the AUC of the HbA2 of ROC was 0.786（95%CI：0.751 ~ 0.821，P < 0.001）and the cut⁃off point was 2.6%，with sensitivity and specificity of 91.1% and 57.4%，respectively. Conclusions The capillary zone electrophoresis is better than the high perfor⁃ mance liquid chromatography when used for diagnosing α⁃thalassemia.

Key words:

capillary zone electrophoresis, HPLC, hemoglobin A2, α?thalassemia trait