Abstract:

Objective By detecting the expression of long⁃chain non⁃coding RNA LINC00467 in ovarian cancer tissues and ovarian cancer cell lines，to preliminarily explore its relationship with the clinicopathological characteristics of ovarian cancer and its biological characteristics in ovarian cancer，and the possible regulatory mechanism of LINC00467 was preliminarily explored. Methods Firstly，based on the gene expression profile interactive analysis database of Cancer Genome Atlas（TCGA），the expression level of LINC00467 in ovarian cancer tissue（Ovarian cancer，OC）was evaluated through bioinformatics analysis；then the possible miRNA targets of LINC00467 were predicted using the Starbase database. Then quantitative real ⁃time PCR（qPCR）was used to detect the expression of LINC00467 in 66 pairs of ovarian cancer tissues and normal ovarian tissues，and analyze its relationship with the clinicopathological characteristics of ovarian cancer. Furthermore，after reducing the expres⁃ sion of LINC00467 through gene silencing technology，CCK⁃8 experiment，colony formation experiment，Transwell migration and invasion experiment were used to determine the effect of LINC00467 on the biological characteristics of ovarian cancer cells. The binding relationship between LINC00467 and miR⁃302a⁃3p was detected by dual⁃lucif⁃erase reporter gene，and the expression of miR⁃302a⁃3p was detected after reducing the expression of LINC00467. Results The bioinformatics analysis showed that the expression of LINC00467 in ovarian cancer tissue（OC）in the Cancer Genome Atlas（TCGA）database was significantly higher than that of the normal control group；quantitative real⁃time PCR（qPCR）indicated that the expression of LINC00467and miR⁃302a⁃3p in 66 cases of OC group was significantly higher than that of the normal ovarian group. The expression of LINC00467in ovarian cancer cell lines was also significantly higher than that in human ovarian epithelial cell lines（HOSEpiC），which verified the results revealed by bioinformatics analysis. Further analysis showed that the expression level of LINC00467 was positively correlated with the pathological grade of ovarian cancer，lymph node metastasis and FIGO staging. The results of survival analysis showed that the overall survival of OC patients with low expression of LINC00467 was better than that of OC patients with high expression of LINC00467. After silencing the expression of LINC00467，the proliferation activity，colony forming ability，migration and invasion ability of ovarian cancer cells were significantly inhibited. Conclusion The long⁃chain non⁃coding RNA LINC00467 is highly expressed in ovarian cancer tissues and cells， which is related to the proliferation and metastasis of ovarian cancer cells，and positively related to the poor prognosis of patients with ovarian cancer. Its mechanism may be that LINC00467 targets and regulates miR⁃302a⁃3p.

Key words:

long non?coding RNA, LINC00467, ovarian cancer, survival analysis