Abstract:

Objective For the purpose of developing a barrier membrane with osteoimmunomodulatory property，this study prepared electroactive biomembranes and clarified its regulatory effects on macrophage polar⁃ ization typing，and further explored the effect of electroactive biomembrane⁃mediated macrophage polarization on osteogenic differentiation. Methods Preparing charged poly（vinylidene fluoride trifluoroethylene）［P（VDF⁃TrFE）］ membrane. A co⁃culture system of P（VDF⁃TrFE）membrane，macrophages，and bone marrow mesenchymal stem cells（BMSCs）was constructed. Uncharged P（VDF ⁃TrFE）membrane was set as a control group. Flow cytometry and PCR techniques were used to detect the phenotype of macrophages on the charged P（VDF⁃TrFE）membrane. Alkaline phosphatase（ALP）and alizarin red staining techniques were used to detect the osteogenic activity of BM⁃ SCs under the stimulation of P（VDF ⁃ TrFE）membrane ⁃ macrophage conditioned medium. Results Charged P （VDF⁃TrFE）membrane had good electrical stability. Macrophages showed high expression of M2 characteristic protein CD206 on the charged membrane，PCR results also showed that Arg⁃1 and IL⁃10 had higher expression on the charged membrane（P＜0.05），and BMSCs produced more ALP and mineralized nodules in the charged membrane⁃macrophage environment. Conclusion The electroactive P（VDF⁃TrFE）membrane can effectively pro⁃ mote the polarization of macrophages to the M2 phenotype. Under its mediated immune environment，BMSCs show high osteogenic activity，which is of great significance to the regeneration and repair of bone defects. 

Key words:

biological materials, macrophages, osteoimmunology, osteogenic differentiation, im?munomodulatory property