老年多重耐药菌感染肺炎患者miR-127-5p、miR-127、miR-155表达水平及其与免疫相关细胞因子的相关性

doi:10.3969/j.issn.1006-5725.2025.03.012

Abstract

Abstract:

Objective To explore the expression levels of ribonucleic acid （miR）-127-5p， miR-127 and miR-155 in elderly patients with pneumonia infection by multidrug-resistant bacteria and their correlation with immune-related cytokines. Methods 65 elderly patients with pneumonia infection by multidrug-resistant bacteria， 80 healthy physical examination persons and 80 elderly patients with common pneumonia in our hospital from June 2020 to June 2022 were retrospectively selected as the observation group， the control group A and the control group B， respectively. The expression levels of miR-127-5p， miR-127， miR-155， interferon-γ （IFN-γ）， interleukin （IL-4）， IL-17， and transforming growth factor-β （TGF-β） in serum samples were detected and compared among the three groups. Pearson correlation analysis analyzed the correlation between miR-127-5p， miR-127， miR-155 and IFN-γ， IL-4， IL-17， TGF-β， and the Receiver operating characteristic curve （ROC） analyzed the diagnostic value of miR-127-5p， miR-127， miR-155 alone or in combination. Results The serum levels of miR-127-5p and IFN-γ in the control group B and the observation group were lower than those in the control group A， and the both in the observation group were lower than the both in the control group B （P < 0.05）. The serum levels of miR-127， miR-155， IL-4， IL-17， and TGF-β in the control group B and observation group were higher than those in the control group A， and those in the observation group were higher than those in the control group B （P < 0.05）. There was a positive correlation between serum miR-127-5p and serum IFN-γ levels （r = 0.463， P < 0.05）， serum miR-127 and IL-4， IL-17 or TGF-β levels （r = 0.306， 0.273 or 0.330， P < 0.05）， and serum miR-155 and IL-4， IL-17 or TGF-β levels （r = 0.410， 0.453 or 0.385， P < 0.05）， and as well as a negative correlation between serum miR-127-5p and IL-4， IL-17 or TGF-β （r = -0.378， -0.396 or -0.408， P < 0.05）， serum miR-127 or miR-155 and IFN-γ levels （r = -0.267 or -0.513， P < 0.05）. Serum miR-127-5p， miR-127 and miR-155 expression levels alone and in combination have a certain diagnostic value in elderly patients with pneumonia infection by multi-drug resistant bacteria， and the area under ROC curve （AUC） values were 0.811， 0.733， 0.817 and 0.906， and the sensitivities were 90.77%， 60.00%， 67.69%， 84.62%， and the specificities were 66.87%， 80.62%， 83.75%， 83.18%. Conclusion MiR-127-5p， miR-127 and miR-155 were differentially expressed in the elderly patients with pneumonia infection by multi-drug resistant bacteria， and were correlated with immune-related cytokines to a certain extent， and the combination of the three has a good diagnostic value， thus may providing a new target for clinical diagnosis of this disease.

Key words: elderly patients, pneumonia, multi-drug resistant bacteria, miR-127-5p, miR-127, miR-155, immune factor, correlation

CLC Number:

R563.1

Yanqing MAO,Ting FU,Juan WANG,Ya′nan WANG,Jie. LI. Expression levels of miR⁃127⁃5p， miR⁃127 and miR⁃155 in elderly patients with pneumonia infection by multidrug⁃resistant bacteria and their correlation with immune⁃related cytokines[J]. The Journal of Practical Medicine, 2025, 41(3): 385-390.

Figures/Tables 5

Tab.1

Tab.2

Tab.3

Tab.4

Fig.1

References 26

1	VAN DUIN D， PATERSON D L. Multidrug-resistant bacteria in the community： an update［J］. Infect Dis Clin North Am， 2020， 34（4）： 709-722. doi:10.1016/j.idc.2020.08.002 doi: 10.1016/j.idc.2020.08.002
2	刘欣，曹雯雯，白冰. TNF-α、NLRP3、CRP/ALB与老年碳青霉烯类耐药肺炎克雷伯菌感染病情程度的关系及对预后的影响［J］. 实用医学杂志， 2021， 37（24）： 3174-3178.
3	ZHANG F， ZHOU Y， DING J. The current landscape of microRNAs （miRNAs） in bacterial pneumonia： opportunities and challenges［J］. Cell Mol Biol Lett， 2022， 27（1）： 70. doi:10.1186/s11658-022-00368-y doi: 10.1186/s11658-022-00368-y
4	FANG X， ZHU L， WU Q， et al. The influence of mdr1 expression regulated by mir-138 through trps1 signaling pathway on multidrug resistance of osteosarcoma and formation of bacterial infection biofilm［J］. Cell Mol Biol （Noisy-le-grand）， 2023， 69（5）： 51-57. doi:10.14715/cmb/2023.69.5.9 doi: 10.14715/cmb/2023.69.5.9
5	LIU H， BAI C， XIAN F， et al. A high-calorie diet aggravates lps-induced pneumonia by disturbing the gut microbiota and th17/treg balance［J］. J Leukoc Biol， 2022， 112（1）： 127-141. doi:10.1002/jlb.3ma0322-458rr doi: 10.1002/jlb.3ma0322-458rr
6	CHEN C， LIN S， ZHOU L， et al. MicroRNA-127-5p attenuates severe pneumonia via tumor necrosis factor receptor-associated factor 1［J］. Exp Ther Med， 2020， 20（3）： 2856-2862.
7	ZHANG Y， ZHAO P， LI S， et al. Circscaper knockdown attenuates il-1beta-induced chondrocyte injury by mir-127-5p/tlr4 axis in osteoarthritis［J］. Autoimmunity， 2022， 55（8）： 577-586. doi:10.1080/08916934.2022.2103798 doi: 10.1080/08916934.2022.2103798
8	TIAN K， XU W. Mir-155 regulates th9 differentiation in children with methicillin-resistant staphylococcus aureus pneumonia by targeting sirt1［J］. Hum Immunol， 2021， 82（10）： 775-781. doi:10.1016/j.humimm.2021.07.002 doi: 10.1016/j.humimm.2021.07.002
9	PARK C M， KIM W， LEE E S， et al. Comparison of frailty index to pneumonia severity measures in older patients with pneumonia［J］. J Am Med Dir Assoc， 2022， 23（1）： 165-169. doi:10.1016/j.jamda.2021.08.044 doi: 10.1016/j.jamda.2021.08.044
10	施毅. 中国成人医院获得性肺炎与呼吸机相关性肺炎诊断和治疗指南（2018年版）［J］. 中华结核和呼吸杂志， 2018， 41（4）： 255-280.
11	李春辉，吴安华. 医疗机构耐药菌MDR、XDR、PDR的国际标准化定义专家建议（草案）［J］. 中国感染控制杂志， 2011， 10（3）： 238-240.
12	卜春红，段立娟，付强，等. ICU老年脑卒中相关性肺炎患者感染病原菌分布及多重耐药菌感染危险因素［J］. 中国老年学杂志， 2022， 42（14）： 3484-3486.
13	唐秋萍，王远芳，袁余，等. 2019-2023年6428例肿瘤患者病原菌分布与耐药性监测报告［J］. 四川大学学报（医学版）， 2024， 55（5）： 1264-1271.
14	陈慧玲，张媛，徐敏，等. 医养结合机构老年坠积性肺炎患者多重耐药菌分布及感染的危险因素［J］. 中国老年学杂志， 2020， 40（16）： 3425-3427.
15	ZHANG D， PAN F， ZHU M， et al. Exosomes derived mir-362 exacerbates pneumonia by increasing interleukin-6 via targeting ventx［J］. Environ Toxicol， 2023， 38（10）： 2298-2309. doi:10.1002/tox.23867 doi: 10.1002/tox.23867
16	WANG J， YANG Z， HE X， et al. Dnm3os/mir-127-5p/cdh11， activates wnt3a/beta-catenin/lef-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis［J］. Noncoding RNA Res， 2024， 9（2）： 294-306. doi:10.1016/j.ncrna.2024.01.008 doi: 10.1016/j.ncrna.2024.01.008
17	ZHU S， FANG Z. Microrna-127-3p inhibits cardiomyocyte inflammation and apoptosis after acute myocardial infarction via targeting cdkn3［J］. Int Heart J， 2023， 64（6）： 1133-1139. doi:10.1536/ihj.23-379 doi: 10.1536/ihj.23-379
18	SHI Y， LI Z， LI K， et al. Mir-155-5p accelerates cerebral ischemia-reperfusion inflammation injury and cell pyroptosis via dusp14/ txnip/nlrp3 pathway［J］. Acta Biochim Pol， 2022， 69（4）： 787-793.
19	钟婉红，郝金香，黄一桂，等. 老年重症肺炎miR-146a和miR-127与miR-21表达水平及其预测价值［J］. 中华医院感染学杂志， 2024， 34（11）： 1626-1630.
20	XU Y， ZHANG C， CAI D， et al. Exosomal mir-155-5p drives widespread macrophage m1 polarization in hypervirulent klebsiella pneumoniae-induced acute lung injury via the msk1/p38-mapk axis［J］. Cell Mol Biol Lett， 2023， 28（1）： 92-98. doi:10.1186/s11658-023-00505-1 doi: 10.1186/s11658-023-00505-1
21	ZHENG X L， GU W J， ZHANG F， et al. Exosomal mir-127-5p from bmscs alleviated sepsis-related acute lung injury by inhibiting neutrophil extracellular trap formation［J］. Int Immunopharmacol， 2023， 123（1）： 110759-110764. doi:10.1016/j.intimp.2023.110759 doi: 10.1016/j.intimp.2023.110759
22	张晴. 某三甲医院鲍曼不动杆菌流行特征及其多重耐药菌感染危险因素分析［D］. 蚌埠：蚌埠医学院， 2023.
23	WANG J， ZHANG Z Q， GIGLIOTTI F， et al. Ifn-gamma limits immunopathogenesis but delays fungal clearance during pneumocystis pneumonia［J］. J Immunol， 2023， 211（9）： 1397-1405. doi:10.4049/jimmunol.2300460 doi: 10.4049/jimmunol.2300460
24	WU Z， MEHRABI NASAB E， ARORA P， et al. Study effect of probiotics and prebiotics on treatment of ova-lps-induced of allergic asthma inflammation and pneumonia by regulating the tlr4/nf-kb signaling pathway［J］. J Transl Med， 2022， 20（1）： 130-135. doi:10.1186/s12967-022-03337-3 doi: 10.1186/s12967-022-03337-3
25	YING H， FANG M， HANG Q Q， et al. Pirfenidone modulates macrophage polarization and ameliorates radiation-induced lung fibrosis by inhibiting the tgf-beta1/smad3 pathway［J］. J Cell Mol Med， 2021， 25（18）： 8662-8675. doi:10.1111/jcmm.16821 doi: 10.1111/jcmm.16821
26	CHENG L， LI Y， LUO Y， et al. Decreased th1 cells and increased th2 cells in peripheral blood are associated with idiopathic inflammatory myopathies patients with interstitial lung disease［J］. Inflammation， 2023， 46（1）： 468-479. doi:10.1007/s10753-022-01747-5 doi: 10.1007/s10753-022-01747-5

组别	例数	miR-127-5p	miR-127	miR-155
对照A组	80	1.56 ± 0.31	0.72 ± 0.22	0.69 ± 0.20
对照B组	80	1.23 ± 0.27	1.24 ± 0.35	1.04 ± 0.28
观察组	65	1.05 ± 0.20	1.48 ± 0.49	1.29 ± 0.34
t₁ 值		7.180	11.251	9.098
P₁ 值		< 0.05	< 0.05	< 0.05
t₂ 值		11.463	12.424	13.223
P₂ 值		< 0.05	< 0.05	< 0.05
t₃ 值		4.469	3.434	4.856
P₃ 值		< 0.05	< 0.05	< 0.05
F值		68.770	86.022	87.788
P值		< 0.05	< 0.05	< 0.05

组别	例数	IFN-γ/（ng/L）	IL-4/（ng/L）	IL-17/（ng/L）	TGF-β/（μg/L）
对照A组	80	71.16 ± 13.28	10.08 ± 3.10	45.52 ± 7.38	0.25 ± 0.08
对照B组	80	63.42 ± 10.69	15.32 ± 4.23	68.41 ± 8.41	0.76 ± 0.14
观察组	65	54.39 ± 11.23	18.11 ± 4.35	92.83 ± 9.55	1.21 ± 0.22
t₁ 值		4.056	8.937	18.298	28.290
P₁ 值		< 0.05	< 0.05	< 0.05	< 0.05
t₂ 值		8.096	12.955	33.646	36.217
P₂ 值		< 0.05	< 0.05	< 0.05	< 0.05
t₃ 值		4.949	3.900	16.361	14.951
P₃ 值		< 0.05	< 0.05	< 0.05	< 0.05
F值		36.067	80.406	567.548	719.177
P值		< 0.05	< 0.05	< 0.05	< 0.05

指标	截断值	AUC值	95% CI	敏感度(%)	特异度(%)
miR-127-5p	≤ 1.23	0.811*	0.754 ~ 0.806	90.77	66.87
miR-127	> 1.29	0.733*	0.670 ~ 0.790	60.00	80.62
miR-155	> 1.13	0.817*	0.760 ~ 0.865	67.69	83.75
联合	-	0.906	0.860 ~ 0.940	84.62	83.18

[1]	Hao WANG,Xiaoyi LU,Wenjie. WANG. Clinical efficacy of concurrent chemoradiotherapy followed by sequential sintilimab immunotherapy in elderly patients with locally advanced esophageal cancer [J]. The Journal of Practical Medicine, 2025, 41(3): 365-370.
[2]	Lei LEI,Yun HU,Di ZHANG,Xinyu HUANG,Yu PENG. Correlation of serum ferritin and 25⁃hydroxyvitamin D with cervical vascular plaque and cardiac metabolism index in elderly patients with nonalcoholic fatty liver disease [J]. The Journal of Practical Medicine, 2025, 41(1): 78-83.
[3]	Jingcai WANG,Chunyan GUO,Lixin YANG,Xiaoqing. JING. Correlation of serum RAGE and HMGB1 expression with the occurrence of acute respiratory distress syndrome and IFN⁃γ/IL⁃4 ratio in patients with severe pneumonia [J]. The Journal of Practical Medicine, 2024, 40(4): 515-520.
[4]	Lihong DING,Shijia GENG,Yujie WANG. Effects of wedelobata on apoptosis and secretion of inflammatory cytokines in the alveolar epithelium infected by Streptococcus pneumonia [J]. The Journal of Practical Medicine, 2024, 40(3): 316-320.
[5]	Hongmei ZHU,Yongjiu HUANG,Jiusheng CHU,Xiuhong. PANG. Expression level of neutrophil/lymphocyte ratio and monocyte/high density lipoprotein cholesterol ratio in patients with sudden deafness and their correlation with prognosis [J]. The Journal of Practical Medicine, 2024, 40(24): 3503-3508.
[6]	Qianqian CHEN,Qiuyu LIN,Xiangyun ZHANG,Dufei. ZHANG. Relationship between macrolide⁃resistant mycoplasma pneumoniae infection and refractory Mycoplasma pneumoniae pneumonia in children [J]. The Journal of Practical Medicine, 2024, 40(22): 3190-3195.
[7]	Xinpan LI,Yi FANG,Jun QIU. Predictive value of CD3⁺/CD4⁺T lymphocyte level and neutrophil to lymphocyte ratio in radiation pneumonia [J]. The Journal of Practical Medicine, 2024, 40(20): 2923-2928.
[8]	Guojiang YIN,Bixi LI,Pengxiao WEI,Yuqin YAN,Xiaoyang SONG,Kun LI. Effect of anterior quadratus lumborum block at the lateral supra⁃arcuate ligament on postoperative analgesia and inflammatory response in elderly patients undergoing robot⁃assisted radical prostatectomy [J]. The Journal of Practical Medicine, 2024, 40(2): 202-206.
[9]	Xing HU,Qingrong LI,Jiang LI,Wei HE,Ping′an HE,Mei LV,Xu. YANG. The Nomogram model was established for the risk assessment of intestinal colonization with neonatal CRKP [J]. The Journal of Practical Medicine, 2024, 40(2): 231-236.
[10]	Yao ZHANG,Yingxin HUO,Wei ZHAO,Rongjie TANG,Qiufang. LIAN. The relationship between blood uric acid levels and non⁃alcoholic fatty liver disease in patients with type H hypertension [J]. The Journal of Practical Medicine, 2024, 40(18): 2561-2565.
[11]	Hongmei LIU,Huahong ZHOU,Xiangjiu CHEN,Guobao HONG,Xiongbin WU,Yanjuan LIANG,Chunting LI,Meidi ZHENG,Yueqin LAI,Fanna. LIU. Association of frailty with anxiety and depression in patients on maintenance hemodialysis [J]. The Journal of Practical Medicine, 2024, 40(18): 2612-2617.
[12]	Tingting YANG,Yidan HUANG,Rongrong YANG,Ying YANG,Jing. ZHANG. Study on the relationship between serum LRG1， LDH with periodontal indexes and periodontal lesions in patients with type 2 diabetes and chronic periodontitis [J]. The Journal of Practical Medicine, 2024, 40(16): 2250-2255.
[13]	Ying LI,Yan LUO,Wenquan LI,Weifeng. LUO. Expression of miR⁃34c and miR⁃29b in peripheral blood of patients with sensorineural deafness and clinical significance [J]. The Journal of Practical Medicine, 2024, 40(15): 2105-2109.
[14]	Yuhong ZHAN,Xinjie SHAN,Jun. ZHOU. MiR⁃155 regulates inflammatory responses and Th17/Treg imbalances in rheumatoid arthritis through the SOCS1/STAT3 pathway [J]. The Journal of Practical Medicine, 2024, 40(13): 1791-1796.
[15]	Fei CHAI,Zhenwen CHEN,Hongyan LIU,Su LI,Yuanyuan GONG,Yingying. ZHAO. Expression level of serum UBE2C and TRIM27 in patients with endometrial cancer and their correlation with pathological parameters [J]. The Journal of Practical Medicine, 2024, 40(13): 1808-1813.

Expression levels of miR⁃127⁃5p， miR⁃127 and miR⁃155 in elderly patients with pneumonia infection by multidrug⁃resistant bacteria and their correlation with immune⁃related cytokines

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 26

Related Articles 15

Recommended Articles

Metrics

Comments

指标	IFN-γ		IL-4		IL-17		TGF-β
指标	r值	P值	r值	P值	r值	P值	r值	P值
miR-127-5p	0.463	< 0.05	-0.378	< 0.05	-0.396	< 0.05	-0.408	< 0.05
miR-127	-0.267	< 0.05	0.306	< 0.05	0.273	< 0.05	0.330	< 0.05
miR-155	-0.513	< 0.05	0.410	< 0.05	0.453	< 0.05	0.385	< 0.05