大黄素上调Sirt2减轻脂多糖致RAW264.7细胞的氧化应激反应

doi:10.3969/j.issn.1006-5725.2024.13.004

Abstract

Abstract:

Objective The aim of this study was to investigate the impact and mechanisms of emodin on oxidative stress response in lipopolysaccharide （LPS）-induced murine mononuclear macrophages （RAW264.7）. Methods Involved the use of LPS， RAW264.7 cells， and emodin. Experimental groups included a control group， LPS（1 μg/mL） group， and LPS（1 μg/mL） + emodin （15 μmmol/L） pretreatment group. Aldehyde malondialdehyde （MDA） content， intracellular reactive oxygen species （ROS） levels， and silent information regulator 2（Sirt2） expression were evaluated at 6， 12， and 18 hours after LPS exposure. Additionally， RAW264.7 cells were pretreated with Sirt2 inhibitor AGK2 （20 μmol/L） followed by LPS stimulation， and the above-mentioned parameters were assessed at 6 hours. Results Compared to the control group， MDA content， ROS levels， Sirt2 mRNA， and protein expression in RAW264.7 cells in the LPS group increased at all time points （all P < 0.05）. At 6 and 18 hours， MDA content and ROS levels in RAW264.7 cells in the LPS + emodin group decreased significantly （all P < 0.05）， while at 12 hours， ROS levels were lower in the LPS group compared to the LPS + emodin group （P < 0.05）. Sirt2 mRNA and protein levels significantly increased at all time points （all P < 0.05） compared to the LPS group. In the LPS + emodin + AGK2 group， Sirt2 mRNA and protein levels decreased， and MDA content and ROS levels increased compared to the LPS + emodin group （all P < 0.05）. Conclusion LPS-induced oxidative stress in RAW264.7 cells and emodin attenuate LPS-induced oxidative stress in RAW264.7 cells through Sirt2.

Key words: sepsis, macrophages, oxidative stress, emodin, Sirt2

CLC Number:

R441.8

Chun LONG,Hongying BI,Changzhen YANG,Jiakai WANG,Yan TANG,Xu. LIU. Emodin upregulates the Sirt2 to attenuate LPS-induced oxidative stress response in RAW264.7 cells[J]. The Journal of Practical Medicine, 2024, 40(13): 1785-1790.

Figures/Tables 8

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References 23

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基因	引物序列（5′-3′）
GAPDH	F：GGTTGTCTCCTGCGACTTCA
GAPDH	R：TGGTCCAGGGTTTCTTACTCC
Sirt2	F：ACATAGACACGCTGGAACGA
Sirt2	R：CACCTGGGAGTTGCTTCTGA

组别	例数	MDA含量（nmol/gprot）	ROS相对生成量（%）
Control	3	2.03 ± 0.93	2.83 ± 0.64
LPS 6 h	3	5.14 ± 0.32^*	13.57 ± 0.55^*
LPS 12 h	3	4.78 ± 0.19^*	38.18 ± 1.82^*
LPS 18 h	3	5.09 ± 0.66^*	48.09 ± 3.24^*
LPS+Emodin 6 h	3	2.35 ± 0.74^#	5.01 ± 0.65^#
LPS+Emodin 12 h	3	2.76 ± 1.03	13.09 ± 1.93^*#
LPS+Emodin 18 h	3	2.35 ± 0.88^#	12.67 ± 0.59^*#

组别	例数	MDA含量（nmol/gprot）	ROS相对生成量（%）
Control	3	3.02 ± 0.85	3.01 ± 0.37
LPS	3	6.91 ± 0.77^*	40.58 ± 4.82^*
LPS+Emodin	3	3.82 ± 0.7^#	5.73 ± 0.47^#
LPS+Emodin+AGK2	3	5.44 ± 1.49^&	13.34 ± 1.93^#&

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Emodin upregulates the Sirt2 to attenuate LPS-induced oxidative stress response in RAW264.7 cells

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