Abstract:

Objective To evaluate the effect of Diosmetin on proliferation，apoptosis，colony formation， migration，adhesion and stemness of PC⁃3cells. Methods The effect of Diosmetin on PC⁃3 cells proliferation was detected using CCK⁃8 assay，and the half maximal inhibitory concentration（IC50）value of Diosmetin was calculated. Cell morphology was observed under a microscope，and the apoptotic⁃related proteins were detected using WB as aforementioned. The ability of colony ⁃forming，adherence and migration of PC ⁃3 cells were determined by colony formation assay，cell adhesion experiment and Transwell experiment，respectively. Results Diosmetin inhibited the growth of PC⁃3 cells in a dose⁃dependent manner，with statistically significant difference（P < 0.05），and the results showed an IC50 value of 10 μg/mL. At the same time，Diosmetin induced PC⁃3 cell apoptosis，western blot analysis demonstrated that the expression of the apoptosis⁃associated genes caspase⁃8，Bax was upregulated while Bcl⁃2 was downregulated. In addition，Diosmetin suppressed PC⁃3 cells colony formation，adhesion，migration and sphere ⁃formingabilities，with statistically significant the difference（P < 0.05）. Conclusion Diosmetin inhibited proliferation，colony formation，adhesion，migration abilities and stemness of PC⁃3 cellsin vitro. Meanwhile，Dios⁃ metin induces apoptosis of PC⁃3 cells.

Key words:

Diosmetin, prostate cancer, bone metastasis, Bax, Bcl2, cancer stem cells