Abstract:

Objective To explore the effects of neural stem cells（NSCs）carrying bovine parvovirus bpV（PIC）combined with neurotrophin 3（NT ⁃ 3）chitosan scaffold on the nerves repair after traumatic brain injury（TBI）. Methods 90 rats were randomly divided into control group，TBI group，TBI +NSCs group，TBI + bpV（PIC）⁃NSCs group，TBI+NT⁃3 chitosan group and TBI+bpV（PIC）⁃NSCs+NT⁃3 Group（n = 15）. The TBI modelwas constructed by impact，and NSCs，bpV（PIC）⁃NSCs，NT⁃3 chitosan or NT⁃3 chitosan loaded with bpV（PIC）⁃NSCs were implanted after impact. Neural function was evaluated by mNSS score and water maze test. HE stainingand BrdU staining were used to evaluate brain tissue damage and neuron regeneration. Results The mNSS score，the degree of brain damage，and the number of new neurons in the TBI group were significantly higher than thoseof the control group，and the target quadrant residence time and the number of crossing platforms were significantlylower than those of the control group（P < 0.05）. The mNSS score and the degree of brain damage in the TBI+NSCsgroup were significantly lower than those in the TBI group，while the target quadrant residence time，the number ofcrossing platforms and the number of new neurons were significantly higher than those in the TBI group（P < 0.05）.The mNSS score and the degree of brain damage in the TBI+bpV（PIC）⁃NSCs group were significantly lower thanthose in the TBI+NSCs group，while the target quadrant residence time，the number of crossing platforms，and thenumber of newborn neurons were significantly higher than those in the TBI+NSCs group（P < 0.05 ）. The mNSSscore and the degree of brain damage in the TBI+bpV（PIC）⁃NSCs+NT⁃3 group were significantly lower than thosein the TBI+bpV（PIC）⁃NSCs group，while the target quadrant residence time，the number of crossing platforms，and the number of newborn neurons were significantly higher In the TBI+bpV（PIC）⁃NSCs group（P < 0.05）.Conclusion The combination of bpV（PIC）⁃NSCs and NT⁃3 chitosan scaffold can significantly promote nerverepair in TBI rats.

Key words:

traumatic brain injury, neural stem cells, bovine parvovirus, chitosan scaffold