The Journal of Practical Medicine ›› 2021, Vol. 37 ›› Issue (15): 1921-1927.doi: 10.3969/j.issn.1006⁃5725.2021.15.003

• Basic Research • Previous Articles     Next Articles

Inhibition of apoptosis of CD4 + T cells in hepatitis B cirrhosis through inducing mitophagy by Jiangan granules

LI Yuan*，LONG Fuli，ZHANG Kan，GUAN Zhijie，YAO Fan，PENG Yunhe，SHU Faming，TANG Chunhui，MAO Dewen，HUANG Guye.   

  1.  Department of Hepatic Diseases，the First Affiliated Hospital of Guangxi Uni⁃ versity of Chinese Medicine，Nanning 530023，China；* Guangxi Key Laboratory of Translational Medicine for Treat⁃ ing High⁃Incidence Infectious Diseases with Integrative Medicine，Nanning 530023，China

  • Online:2021-08-10 Published:2021-08-10
  • Contact: HUANG Guye E⁃mail：huangguye@163.com
  • Supported by:


Abstract:

Objective Based on the PINK1/Parkin/ROS pathway，to explore the effect of Jiangan Gran⁃ ules on inhibiting the apoptosis of CD4+ T cells in liver cirrhosis and its mechanism. Methods Hepatitis B cirrho⁃ sis（HBC）patients were divided into control group and observation group，and healthy people were selected as the normal group，with 50 cases in each group. The control group was treated with entecavir，and the observation group with Jiangan Granules combined with entecavir. CD4 + T cells in the peripheral blood of each group were screened by magnetic bead sorting method. CD4+ T cells in the normal group were divided into control group，H2O2 group and H2O2+CCCP（Mitophagy inducer）group. The contents of ROS，SOD and MDA were detected by ELISA. The expression of PINK1，Parkin，LC3⁃Ⅱ，Bax and Bcl⁃2 was detected by Western blot. The mitochondrial mem⁃ brane potential and apoptosis rate were detected by flow cytometry. Results CCCP could reverse the promoting ef⁃ fect of H2O2 on ROS and apoptosis rate of CD4+ T cell and the inhibiting effect of LC3⁃Ⅱ expression（P<0.05）. Ji⁃ angan Granules combined with entecavir could down ⁃ regulate ROS，MDA and SOD in serum，while up ⁃ regulate the expression of PINK1，Parkin，LC3⁃Ⅱ and Bcl⁃2 in CD4+ T cells and it could also down⁃regulate the expression of Bax，increase mitochondrial membrane potential and reduce the apoptosis rate（P<0.05）. The effect was better than that of entecavir alone. Conclusion Upregulation of CD4 + T cell mitophagy level could inhibit oxidative stress and apoptosis. Jiangan Granules could inhibit the apoptosis of CD4+ T cell in compensated patients with HBCby inducing mitophagy.

Key words:

Jiangan granules, mitophagy, CD4+ T cells, hepatitis B cirrhosis, oxidative stress