关键词: 循环肿瘤细胞, 前列腺癌, 诊断价值

Abstract:

Objective To investigate the clinical value of circulating tumor cells（CTCs）in the diagnosisand treatment of prostate cancer patients. Methods From September 2016 to September 2019，78 patients withprostate cancer who were treated in the urology department of the Second Affiliated Hospital of Zhengzhou Universitywere selected as the research group and 35 healthy subjects were used as the control group. The amount of 7.5 mLvenous blood was collected from each subject of both groups. CanPatrolTM technology was used to enrich，isolate，and count CTCs. According to CTCs count，prostate cancer patients were sub⁃divided into CTCs positive group andCTCs negative group，and their age，blood routine，biochemical indicators，prostate specific antigen（PSA），abdominal CT，prostate nuclear magnetic resonance（MRI），bone scan，Gleason were recorded at the same time.The correlations between the above indicators and the number of CTCs were analyzed. The diagnostic value of PSAlevel and CTCs level in prostate cancer was evaluated by the four⁃grid. Results The positive rate of CTCs in thepatients with metastatic prostate cancer was significantly higher than that in the patients without metastasis（P<；0.05）. The diagnostic sensitivity of CTCs and PSA for prostate cancer was 88.46% and 85.89%，respectively，thespecificity was 74.28% and 65.71%，respectively，and the accuracy was 84.07% and 79.64%，respectively. Thelevels of HB and ALB in the CTCs⁃positive group were significantly lower than those in the CTCs⁃negative group（P < 0.05），and the levels of PLT，FIB，DD，AKP，and LDH were significantly higher than those in CTCs⁃negativegroup（P < 0.05）. Pearson analysis showed that the CTCs were significantly positively correlated with PSA andGleason scores（r = 0.601，P < 0.05；r = 0.316，P < 0.05）. The PSA levels increased with the worsening stages，with significant differences at stage Ⅱ，stage Ⅲ，stage Ⅳ as compared with stage I，and between stage Ⅳ andstage Ⅱ（P < 0.05）. The number of CTCs also increased with the worsening pathological stages，with statisticaldifferences between stage Ⅳ and stage Ⅱ（P < 0.05）. Conclusion The CTCs levels are correlated with tumorigenesisand metastasis，related serological indicators，Gleason score of prostate cancer，and tumor pathological stage，andthey are of clinical significance in predicting the development of prostate cancer.

Key words: circulating tumor cells, prostate cancer, diagnostic value