实用医学杂志 ›› 2021, Vol. 37 ›› Issue (14): 1789-1794.doi: 10.3969/j.issn.1006⁃5725.2021.14.003

• 基础研究 • 上一篇    下一篇

细胞周期蛋白L1行为的影响对人绒毛膜滋养细胞生物学

杨闪闪1,唐冉冉2,江华2,汤秋勤2   

  1. 1南京医科大学(南京210029);2南京医科大学附属妇产医院(南京市妇幼保健院)(南京210004)

  • 出版日期:2021-07-25 发布日期:2021-07-25
  • 通讯作者: 江华 E⁃mail:jianghua@njmu.edu.cn
  • 基金资助:

    国家自然科学基金项目(编号:81771597,81971405)

Effects of cyclin L1 on biological behavior of human trophoblast cells 

YANG Shanshan*,TANG Ranran,JI⁃ ANG Hua,TANG Qiuqin.    

  1. Nanjing Medical University,Nanjing 210029,China 

  • Online:2021-07-25 Published:2021-07-25
  • Contact: JIANG Hua E⁃mail:jianghua@njmu.edu.cn

摘要:

目的 探讨细胞周期蛋白L1(CCNL1)对人绒毛膜滋养细胞生物学行为的影响及调控机制。方法 以人绒毛膜滋养细胞HTR⁃8/SVneo为模型,根据转染的类型分为CCNL1过表达组,过表达对照组,CCNL1干扰组1,CCNL1干扰组2,CCNL1干扰组3,干扰对照组,采用实时荧光定量(qPCR)及WesternBlot方法检测CCNL1mRNA和蛋白水平的表达变化;CCK⁃8法检测增殖能力;Transwell方法检测细胞侵袭、迁移能力;流式技术检测细胞周期和凋亡情况。结果 过表达CCNL1后,滋养层细胞的迁移、侵袭能力降低(P<0.05);增殖能力降低(P<0.05);细胞周期S期的细胞数减少(P<0.05)。干扰CCNL1后,滋养层细胞的迁移、侵袭能力增强(P<0.05);增殖能力增强(P<0.05)。结论 CCNL1基因可能通过调控滋养层细胞的迁移、侵袭以及增殖能力而影响胎盘的功能。

关键词:

细胞周期蛋白L1, 滋养层细胞, 胎儿生长受限, 细胞增殖, 细胞迁移

Abstract:

Objective To explore the effect of Cyclin L1(CCNL1)on the biological behaviors and regula⁃ tory mechanism of human trophoblast cells. Methods We used HTR ⁃ 8/SVneo as the model and divide it into CCNL1 overexpression group,CCCNL1 overexpression control group,CCNL1 interference group 1,CCNL1 inter⁃ ference group 2,CCNL1 interference group 3 and interference control group. The mRNA expression level of CCNL1 was detected by Quantitative Real⁃time PCR(qPCR);the protein expression level of CCNL1 by Western Blot and cell proliferation by CCK⁃8. Cell migration and invasion were detected by Transwell assay and cell cycle and cell apoptosis by flow cytometry. Results Overexpression CCNL1 significantly decreased the migration and invasion ability of trophoblast cells(P < 0.05),the proliferation ability of trophoblast cells(P < 0.05),and the number of S cells(P < 0.05). Interfered CCNL1 significantly increased the migration and invasion ability of tropho⁃ blast cells(P < 0.05),and the proliferation ability of trophoblast cells(P < 0.05). Conclusion CCNL1 gene may affect placental function by regulating the migration,invasion and proliferation of trophoblast cells.

Key words:

cyclin L1, trophoblast cells, fetal growth restriction, cell proliferation, cell migration