关键词:

子宫内膜异位症, 肠道菌群, 16S rRNA, 免疫失衡

Abstract:

Objective To investigate the differences in gut microbiome among patients with ovarian endo⁃ metriosis，those with deep infiltrating endometriosis，and healthy women，and to predict the gene function of flora. Methods Feces were collected from 14 patients with ovarian endometriosis（OE group，n = 14），12 with deep infiltrating endometriosis（DIE group，n = 12），and healthy women（control group，n = 10）during the period of 2019 to 2020. The common conserved region V3⁃V4 of intestinal flora was sequenced by 16S rRNA amplification and the sequencing results among three groups were analyzed；the gene function of microbiome was predicted. Results The diversity of the flora did not differ significantly among the three groups. Negativicutes and Fusicateni⁃ bacter were significantly decreased while NK4A214_group and Eubacterium_coprostanoligenes_group were signifi⁃ cantly increased in OE group and DIE group. Amino acid metabolism and ABC transporter were enriched in OE group. Conclusions Imbalance of intestinal flora was existed in patients with endometriosis. The development of ovarian endometriosis and deep infiltrating endometriosis may be related to amino acid metabolism and transporters. 

Key words:

endometriosis, gut microbiome, 16S rRNA, immune imbalance