STK4-AS1通过MYG1/Notch信号通路对食管鳞癌恶性生物学行为的影响

doi:10.3969/j.issn.1006-5725.2025.17.008

摘要/Abstract

摘要：

目的探讨STK4-AS1通过MYG1/Notch信号通路抑制食管鳞癌细胞增殖、侵袭以及迁移能力。方法采用实时荧光定量PCR（qRT-PCR）检测食管鳞癌细胞中STK4-AS1表达。通过MTS、划痕愈合实验、Transwell小室侵袭实验检测各组食管鳞癌细胞Eca109和Kyse150的增殖、迁移以及侵袭能力。通过mRNA测序（mRNA-seq）检测STK4-AS1下游作用靶基因。通过KEGG功能富集分析预测可能的生物学过程和信号通路。采用qRT-PCR和Western blot方法检测MYG1以及Notch信号通路下游关键转录因子HES1、HES5、HEY1的mRNA表达和NICD1蛋白质表达。共转染质粒（针对STK4-AS1和MYG1过表达）检测食管鳞癌细胞的Notch信号通路下游关键转录因子HES1、HES5、HEY1的mRNA表达和NICD1蛋白质表达以及食管鳞癌细胞增殖、迁移和侵袭能力的影响。结果 STK4-AS1在食管鳞癌细胞系中表达降低（P <0.01）。过表达STK4-AS1后抑制食管鳞癌细胞Eca109和Kyse150增殖、迁移和侵袭能力（P < 0.05）。过表达STK4-AS1负向调控MYG1的表达（P < 0.01），MYG1在食管鳞癌细胞系中表达升高（P < 0.01）。过表达MYG1可部分逆转STK4-AS1对Eca109和Kyse150细胞恶性生物学行为的影响（P < 0.05），以及Notch信号通路下游关键转录因子HES1、HES5、HEY1的mRNA表达和NICD1蛋白质表达（P < 0.05）。结论 STK4-AS1通过MYG1/Notch信号通路影响食管鳞癌恶性生物学行为。

关键词: 食管鳞状细胞癌, 长链非编码RNA, STK4-AS1, Notch信号通路

Abstract:

Objective To investigate the role of STK4?AS1 in regulating the proliferation， invasion， and migration of esophageal squamous cell carcinoma （ESCC） cells through the MYG1/Notch signaling pathway. Methods Quantitative real?time PCR （qRT?PCR） was used to detect the expression of STK4?AS1 in ESCC cells. MTS assay， wound healing and Transwell assay were conducted to explore the proliferation， migration， and invasion abilities in each group in Eca109 and Kyse150 cells. mRNA sequencing （mRNA?seq） was used to detect the downstream target genes of STK4?AS1. KEGG functional enrichment analyses were used to predict the possible biological processes and signaling pathways. qRT?PCR and western blot were performed to identify mRNA expression of MYG1 and the key downstream transcription factors HES1， HES5， and HEY1 of the Notch signaling pathway， as well as the protein expression of NICD1. Co?transfection plasmids （for over?expressing STK4?AS1 and MYG1） were used to detect the mRNA expression of HES1， HES5， and HEY1 and the protein expression of NICD1 which acted as the key downstream transcription factors in the Notch signaling pathway， as well as the effects on the proliferation， migration， and invasion abilities of ESCC cells. Results The expression of STK4?AS1 was decreased in ESCC cell lines （P < 0.01）. Over?expression of STK4?AS1 inhibited the proliferation， migration and invasion abilities in Eca109 and Kyse150 cells （P < 0.05）. STK4?AS1 negatively regulated the expression of MYG1 （P < 0.01）， and the expression of MYG1 was increased in ESCC cell lines （P < 0.01）. Over?expression of MYG1 could partially reverse the effect of STK4?AS1 on the malignant biological behavior of Eca109 and Kyse150 cells （P < 0.05）， as well as the mRNA expressions of HES1， HES5， and HEY1 and the protein expression of NICD1 （P < 0.05）. Conclusion STK4?AS1 affects the malignant biological behaviors of ESCC through the MYG1/Notch signaling pathway。

Key words: esophageal squamous cell carcinoma, long non?coding RNA, STK4?AS1, Notch signaling pathway

中图分类号:

R735.1

冯博,曹家瑞,李东东,许彦超,马纯政. STK4-AS1通过MYG1/Notch信号通路对食管鳞癌恶性生物学行为的影响[J]. 实用医学杂志, 2025, 41(17): 2661-2669.

Bo FENG,Jiarui CAO,Dongdong LI,Yanchao XU,Chunzheng MA. Role of STK4⁃AS1 in regulating malignant biological behavior of esophageal squamous cell carcinoma through the MYG1/Notch signaling pathway[J]. The Journal of Practical Medicine, 2025, 41(17): 2661-2669.

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