miR-209下调MEK/ERK信号通路抑制胶质瘤U87细胞增殖、增强放疗敏感性的研究

doi:10.3969/j.issn.1006⁃5725.2021.13.005

实用医学杂志 ›› 2021, Vol. 37 ›› Issue (13): 1670-1673.doi: 10.3969/j.issn.1006⁃5725.2021.13.005

miR-209下调MEK/ERK信号通路抑制胶质瘤U87细胞增殖、增强放疗敏感性的研究

YANG Bin，YU Jing，LIU Xiaozhi，CHEN Lei.

天津市第五中心医院神经外科（天津 300450）

出版日期:2021-07-10 发布日期:2021-07-10
基金资助:
天津市滨海新区卫计委课题（编号：2019BWKY028）

miR ⁃ 209 inhibits proliferation of glioma U87 and enhances its radiosensitivity by downregulating signal pathway of MEK/ERK

杨彬，于晶，刘晓智，陈镭

Department of Neurosurgery，Tianjin Fifth Central Hospital，Tianjin 300450，China

Online:2021-07-10 Published:2021-07-10

摘要/Abstract

摘要：

目的探讨miR⁃209下调MEK/ERK信号通路对胶质瘤U87细胞增殖、增强放疗敏感性的影响。方法采用 Real Time PCR 的方法检测胶质瘤 U87 细胞放疗前后 miR⁃209、MEK、ERK mRNA 的表达；通过转染技术使 U87 细胞内过表达 miR⁃209，Western blot、Real time PCR 检测细胞内 MEK、ERK 表达水平。结果放疗后胶质瘤 U87 细胞中 miR⁃209 相对表达降低（t = 2.491，P = 0.037 4），放疗前后 ERK、ERK mRNA的相对表达差异无统计学意义；miR⁃209 mimics转染U87细胞后，MEK mRNA表达减少（0.11 ± 0.05）， ERK mRNA 表达减少（F = 9.891，P = 0.002 9），p⁃MEK、p⁃ERK 表达均降低。miR⁃209 转染后 24、48 h，细胞增殖受到抑制。结论 miR⁃209 通过下调胶质瘤 U87 细胞中 MEK⁃ERK 的表达，能抑制细胞增殖，增强放疗敏感性。

关键词:

胶质瘤, 放疗敏感性, 信号通路

Abstract:

Objective To investigate the effect of miR⁃209 down regulating the MEK/ERK signal pathway on the proliferation and radiosensitivity of glioma U87 cells. Methods The expressions of miR ⁃209，MEK and ERK mRNA in U87 cells before and after radiotherapy were detected by Real Time PCR. After miR⁃209 transfection， the expressions of miR⁃209，ERK and MEK were detected by Western blot and real time PCR. Results After radiotherapy，the relative expression of miR⁃209 in U87 cells decreased（t = 2.491，P = 0.0374），and there was no significant difference in ERK and ERK mRNA expressions between pre⁃ and post⁃radiotherapy. After the miR⁃209 was transfected to the U87 cells，the MEK mRNA expression decreased（0.11 ± 0.05），The ERK mRNA expression decreased（F = 9.891，P = 0.002 9），and the p⁃MEK and p⁃ERK expressions both decreased，and cell prolifera⁃ tion was inhibited. Conclusion miR⁃209 can inhibit cell proliferation and enhance radiosensitivity by down regu⁃ lating the expression of MEK⁃ERK in U87 cells.

Key words:

glioma, radiosensitivity, signal pathway

杨彬, 于晶, 刘晓智, 陈镭 .

miR-209下调MEK/ERK信号通路抑制胶质瘤U87细胞增殖、增强放疗敏感性的研究 [J]. 实用医学杂志, 2021, 37(13): 1670-1673.

YANG Bin, YU Jing, LIU Xiaozhi, CHEN Lei. .

miR ⁃ 209 inhibits proliferation of glioma U87 and enhances its radiosensitivity by downregulating signal pathway of MEK/ERK [J]. The Journal of Practical Medicine, 2021, 37(13): 1670-1673.

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miR-209下调MEK/ERK信号通路抑制胶质瘤U87细胞增殖、增强放疗敏感性的研究

miR ⁃ 209 inhibits proliferation of glioma U87 and enhances its radiosensitivity by downregulating signal pathway of MEK/ERK

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