关键词:

脓毒症, 血红素氧合酶?1, 大鼠, 肺组织, HE 染色, HO?1 mRNA

Abstract:

Objective To study the expression of heme oxidase（HO⁃1）and HO⁃1 mRNA in the lung tissue of septic rats. Methods Forty rats were selected and randomly divided into a sham operation group of 20 rats and a sepsis group of 20 rats；the two groups were decapitated and sacrificed at 20 min before surgery and 2，6 h and 12 h after modeling. HE stained light microscope pathological sections；collected the detection results of HO1⁃1 protein activity level by Western blotting method and the detection results of HO⁃1 mRNA level by Realtime PCR method；constructed sepsis model by rat cecal ligation and perforation（CLP）method；immune group Observe the lung tissue morphology，HO⁃1mRNA and HO⁃1 protein expression at each time point；observe the lung injury tissue morphology and HO ⁃1 level changes in sepsis rats；analyze the corresponding indexes in HO ⁃1 and sepsis And lung function damage. Results The lung tissue morphology of the sham operation group was always in a com⁃ plete state，while the lung tissue morphology of the sepsis group had obvious pathological changes in the lung tissue；the color of HO⁃1 imprints in the sepsis group was very different at each time point. The color of the blot is darkest at 12 h，followed by the lightest color at 6，24 h；while the color of the HO⁃1 blot at the 3 time points of the sham operation group is very light，and the color of the blot at 12 h is the darkest at 12 h like the sepsis group. Followed by the lightest color at 6 and 24 h；the sham operation group has remained stable without significant changes，while the sepsis group has the lowest OD value at 2 h，and then gradually increases. The difference in OD at the 3 time points is statistically significant significance（P < 0.05）；the OD value of rats in the sepsis group was higher than that in the sham operation group at each time point（P < 0.05）. Conclusion The expression of HO⁃1 in the lung tissue of the sepsis group was significantly higher than that in the sham operation group，indicat⁃ ing that HO⁃1 has a significant effect on the pathomorphological changes of the lung tissue of patients with sepsis

lung tissue in the sepsis group The pathomorphological damage of the lung tissue gradually reduced with the increase of the expression of HO⁃1 in the lung tissue，indicating that HO⁃1 has a significant protective effect and improvement effect on the pathological morphology of the lung tissue of patients with sepsis.

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