Cardiac lymphoma is a rare form of extranodal lymphoma with an extremely poor prognosis. Sec⁃ ondary cardiac lymphoma（SCL）is more common than primary cardiac lymphoma and is usually found in patients with disseminated lymphoma or advanced lymphoma. Patients with SCL often experience non ⁃ specific symptoms， which lead to a delay in diagnosis and treatment，and in some cases，SCL cannot be detected until a postmortem. There are no systematic guidelines for the diagnosis and treatment of SCL. The value of early diagnosis and therapy in enhancing survival rates cannot be underestimated. This article outlines the most recent developments in the diag⁃ nosis and treatment of SCL.

Objective To investigate the effect of tetraspanin 1（Tspan1）overexpression on oxaliplatin （OXA）⁃induced apoptosis in human colorectal cancer cell line HCT116 and to analyze its possible mechanism of action. Methods HCT116 cells were transfected with Tspan1 overexpression plasmid and its negative control vec⁃ tor plasmid. The expression levels of Tspan1 mRNA and protein were detected by qRT⁃PCR and Western blot. The transfected HCT116 cells were treated with 14.15 μg/mL OXA or combined with 5 mmol/L autophagy inhibitor 3⁃ MA. MTT assay was used to detect cell proliferation. Flow cytometry was used to detect cell apoptosis. The expres⁃ sion of LC⁃3B protein was detected by immunofluorescence. The expression levels of autophagy⁃related proteins LC⁃ 3，Beclin1 and P62 were detected by Western blot. Results Overexpression of Tspan1 significantly up⁃regulated Tspan1 mRNA and protein expression levels in HCT116 cells，reduced the sensitivity of HCT116 cells to OXA，in⁃ hibited OXA⁃induced apoptosis of HCT116 cells，and enhanced the fluorescence intensity of LC3B protein，up⁃reg⁃ ulated the protein expression level of Beclin1 and the ratio of LC⁃3II/LC⁃3I，down⁃regulated the protein expression level of p62，and antagonized the induction of apoptosis by OXA. However，combined treatment with 3⁃MA re⁃ versed the inhibitory effect of Tspan1 overexpression on OXA ⁃induced apoptosis of HCT116 cells，and increased the sensitivity of cells to OXA. Conclusion Overexpression of Tspan1 antagonizes OXA ⁃ induced apoptosis in HCT116 cells by a mechanism that may be mediated through the induction of cellular autophagy.

Objective To investigate the effect of gene expression by NSUN2 in ovarian cancer cell line A2780 by RNA ⁃seq technology，and to provide a theoretical basis for clarifying the role of NSUN2 in the occur⁃ rence and progression of ovarian cancer. Methods Expression of NSUN2 in A2780 cells was knocked down by lentivirus⁃mediated RNA interference technique. The cells transfected with empty vector were used as control，and the interfering cell lines were screened for differentially expressed genes by high⁃throughput sequencing using RNA⁃ seq technology. KEGG Pathway，GO analysis and GSEA enrichment analysis were used to explore the related sig⁃ naling pathways and biological processes of NSUN2⁃regulated differential genes. Results Compared with the con⁃ trol cells，there were 1 642 differentially expressed genes in the A2780 cell line with NSUN2 knockdown，includ⁃ ing 1 020 up⁃regulated genes and 622 down⁃regulated genes. Through GO analysis，KEGG Pathway and GSEA en⁃ richment analysis，it was found that differentially expressed genes were mainly enriched in hedgehog signaling path⁃ way and P53 signaling pathway，and were involved in viral transcription，translation initiation and regulation. Three key genes related to the P53 signaling pathway（ZMAT3，EI24 and CCND2）were screened out through fur⁃ ther analysis of the genes related to the p53 signaling pathway. The expression results were verified by RT ⁃qPCR and the sequencing results were consistent. Conclusion There are significant differences in gene expression pro⁃ files between A2780 sh⁃NSUN2 group and A2780 sh⁃NC group，and the differential genes are mainly concentrated in P53 signaling pathway. In ovarian cancer，NSUN2 may regulate the occurrence and development of ovarian can⁃ cer by regulating the expression of P53 signaling pathway related factors.

Objective To observe the effect on myocardial H9c2 cells gap junction intercellular communi⁃ cation（GJIC）by rat cardiac fibroblasts（RCFs）after hypothermia hypoxia/reoxygen（H/R）and to explore the pos⁃ sible mechanisms. Methods After co⁃cultured of RCFs cells with H9c2 cells in a 2:1 number ratio by Transwell， cells was randomly divided into 6 groups. The T+AngⅡ group and H/R⁃T+AngⅡ group were added 1.5nM AngⅡ， and the T+ARB group and H/R⁃T+ARB group were added 1nM valsartan. The T group，T+AngⅡ group and T+ ARB group were cultured normally，and the H/R⁃T group，H/R⁃T+AngⅡ and H/R⁃T+ARB group were treated with H/R. The AngⅡ concentration in the culture medium；the expression and phosphorylation of Cx43 and ERK1/2； and GJIC of H9c2 cells were detected. Results Compared with the T group，the expression and phosphorylation of ERK1/2 and Cx43 were decreased（P < 0.05）and GJIC was diminished（P < 0.05）in H9c2 cells in the H/R⁃T and T+Ang II group; he expression and phosphorylation of ERK1/2 and Cx43 were increased（P < 0.05）and GJIC was enhanced（P < 0.05）in H9c2 cells in the T+ARB group. Compared with H/R ⁃T group，he expression and phosphorylation of ERK1/2 and Cx43 were decreased（P < 0.05）and GJIC was diminished（P < 0.05）in H9c2 cells in H/R ⁃T+Ang II group; and the expression and phosphorylation of ERK1/2 and Cx43 were increased（P < 0.05）and GJIC was diminished（P < 0.05）in H9c2 cells in H/R ⁃T+ARB group. Conclusion After H/R，in⁃ creased AngⅡ secretion by RCFs may downregulate Cx43 expression by inhibiting the MAPK/ERK pathway in H9c2 cells，resulting in diminished GJIC between H9c2 cells.

Objective To investigate the effect and molecular mechanism of remimazolam on LPS⁃induced acute lung injury. Methods C57BL/6 mice were divided into normal control group，acute lung injury group，remimazolam pretreatment group and remimazolam control group，After 12 hours，the mice were sacrificed for BALF，serum and lung tissue collection. Lung injury was evaluated by HE，W/D ratio and total protein concentra⁃ tion in BALF. ELISA was used to detected the contents of TNF⁃α，IL⁃6，IL⁃1β，IL⁃10. The M1 macrophage marker iNOS + and the M2 macrophage marker CD206 + in F4/80 + macrophages were detected by flow cytometry and the mRNA and protein expressions of TNF ⁃ α，iNOS，CD206 and Arg1 were detected by real ⁃time quantitative PCR and Western blotting. Results Twelve hours after intratracheal injection of LPSA，the lung injury score，W/D ra⁃ tio and total protein concentration in BALF were significantly higher，the TNF⁃α，IL⁃6，IL⁃1β in BALF and serum was significantly increased，iNOS + macrophage was significantly increased，the mRNA and protein expressions of TNF⁃α and iNOS were significantly increased，as compared with the control group. After activated by remimazol⁃ am，the lung injury score，W/D ratio and total protein concentration in BALF were significantly decreased，the TNF⁃α，IL⁃6，IL⁃1β in BALF and serum was significantly decreased，IL⁃10 was significantly increased，iNOS + macrophage was significantly decreased，CD206 + macrophage was significantly increased，the mRNA and protein expressions of TNF⁃α and iNOS were significantly decreased，the mRNA and protein expressions of CD206 and Arg⁃ 1 were significantly increased，as compared with the LPS group. Conclusion Remimazolam alleviates LPS ⁃in⁃ duced acute lung injury by inhibiting macrophage polarization to M1 phenotype and promoting the macrophage po-larization to M2 phenotype.

Objective To explore the molecular mechanism of arginine methyltransferase 1（CARM1）reg⁃ ulating ferroptosis in malignant biology of lung cancer cells. Methods The expression of CARM1 in normal human bronchial epithelial cells（HBE4）and lung cancer lines（A549，H1299，H1640，HCC827）was detected by west⁃ ern blot. CARM1 sequence or Vector and shRNA sequence against CARM1（shCARM1）and Notch homologue 2 （shNotch2）or negative control shRNA（shNC）were transfected into lung cancer cells. Cell proliferation，migration and invasion were measured by CCK ⁃8 test and Transwell test respectively. RIP ⁃PCR and MeRIP ⁃qPCR analysis were used to explore the mechanism of CARM1. Erastin，a classical ferroptosis inducer，was used to treat lung can⁃ cer cells to determine whether CARM1 could affect the sensitivity of cells to ferroptosis. Results Compared with human normal airway epithelial cell HBE4，CARM1 was significantly over⁃expressed in lung cancer lines（A549， H1299，H1640，HCC827）（P < 0.05）. Compared with Vector group，the proliferation，migration and invasion ability of A549 cells in CARM1 group was significantly increased（P < 0.001），while that of HCC827 cells in shCARM1 group was significantly lower than that in shNC group（P < 0.001）. MeRIP⁃qPCR analysis showed that m6A abundance of Notch2 mRNA increased significantly when CARM1 was overexpressed（P < 0.05）. RIP analy⁃ sis showed that CARM1 overexpression significantly promoted the binding between CARM1 and Notch2 mRNA （P < 0.05）. Notch2 knockdown weakened the proliferation，invasion and migration of A549 cells up⁃regulated by CARM1（P < 0.001）. Conclusion CARM1 is significantly elevated in lung cancer，and plays its carcinogenic role by activating Notch2. In addition，CARM1 can make lung cancer cells insensitive to ferroptosis by stabilizing Notch2.

Objective To investigate the composition in bacterial microbial composition in silicosis pa⁃ tients with different stages. Methods 13 healthy subjects，29 patients with occupational silicosis，including 6 pa⁃ tients with silicosis I，15 patients with silicosis Ⅱ and 8 patients with silicosis Ⅲ. The sputum of four groups of subjects was collected，16S rDNA sequencing technology was used to detect bacterial microorganisms in the spu⁃ tum samples，and the relative abundance of bacterial microorganisms and the difference of microbial diversity in⁃ dex（alpha diversity index，beta diversity index）were analyzed；the subject working characteristic curve（ROC） was drawn to assess whether bacterial microorganisms have the potential of biomarkers. Results The relative abun⁃ dance of Neisseria，Moraxella，Staphylococcus，Corynebacterium，Dolosigranulum and Treponema in sputum sam⁃ ples of subjects（P values < 0.05）. The results of the LEfSe analysis showed that，the increased species in the sili⁃ cosis I group include Pseudomonadales，Neisseriaceae，Moraxellaceae，and Neisseria. The additional species in⁃ cluded in the silicosis Ⅱ group were Staphyloccaceae Bacillaceae，Bacillus，Staphylococcus，and Staphylococ⁃ cus_aureus. The ROC analysis showed that the area using Neisseria relative abundance of silicosis Ⅰ and silicosis Ⅱ was 0.87（95% confidence 0.71～0.99），sensitivity = 100.0%，specificity = 73.3%，Youden index = 0.73， P = 0.01. Conclusion Neisseria may be related to the disease process in patients with silicosisis and have the po⁃ tential as a biomarker for distinguishing the silicosis stage.

Objective To compare the effect of quadratus lumborum block at the lateral supra⁃arcuate liga⁃ ment and subcostal transversus abdominis plane block on postoperative painand recovery quality in patients under⁃ going open partial hepatectomy. Methods Sixty patients undergoing open partial hepatectomy were enrolled and randomly allocated into two groups：a quadratus lumborum block at the lateral supra⁃arcuate ligament group（group Q，n = 30）and a subcostal transversus abdominis plane block group（group T，n = 30）. The cumulative total mor⁃ phine equivalent consumption and numerical rating scale（NRS）scores over the 48 hours postoperative period were recorded. Other outcomes including quality of recovery⁃15（QoR⁃15）scores，Athens insomnia scale（AIS）scores， time to first patient ⁃controlled intravenous analgesia（PCIA），invalid bolus times in PCIA，the rescue analgesia rate and adverse events were also recorded. Results Compared with group T，the cumulative total morphine equiv⁃ alent consumption in postoperative 48 hours and the NRS pain scores in 24 hours after surgery were decreased sig⁃ nificantly in group Q（P < 0.05）. The rescue analgesia rate and invalid bolus times in PCIA were significantly low⁃ er in group Q than in group T（P < 0.05）. Time to first PCIA request were prolonged significantly in group Q than in group T（P < 0.05）. There was a significant increase in QoR⁃15 scores and a significant decrease in AIS scores among patients in group Q compared with those in group T（P < 0.05）. No significant difference in adverse events was observed between the two groups（P > 0.05）. Conclusion Compared with subcostal transversus abdominis plane block，ultrasound⁃guided quadratus lumborum block at the lateral supra⁃arcuate ligament could provide bet⁃ ter postoperative analgesia，reduce perioperative opioid consumption and promote the early recovery quality for pa⁃ tients undergoing open partial hepatectomy.