Objective To compare the perinatal outcomes of cleavage embryo transfer using time⁃lapse（TL）and conventional culture and evaluate the security of TL. Methods Aretrospective analysis on clinical dataof 1，999 IVF/ICSI⁃ET cycles in our center from January 2016 to March 2019 were performed. According to themethod of embryo culture，they weredivided into TL group（n = 871）and conventional group（n = 1 128）and theperinatal outcomes in the two groups were compared. Results There was no significant difference in the twogroups in terms of endometrium，ectopic pregnancy rate，early abortion rate，late abortion rate，multifetation rate，premature birth rate，adverse neonatal outcome，extremely Low birth weight infants，very low birth weight infant，macrosomic infant，gestational weeks，neonatal weight，malformation rate and sex ratio（P > 0.05）. The numbersof transplanted embryos in the TL group were significantly less than those in the conventional group（P < 0.05）.Clinical pregnancy rate，implantation rate，live birth rate and the rate of holding babieshome in the TL group weresignificantly higher than those in the conventional group（P < 0.05）. Conclusion Without adverse effects on peri⁃natal outcomes of offspring，TL can significantly improve IVF/ICSI clinical outcomes. It is a safe and effective meth⁃od for embryo culture.