Objective To investigate different HBV infection patterns of couples on the pregnancy and neonatal outcomes in the intrauterine insemination（IUI）. Methods A retrospective analysis of IUI treatment from January 2016 to June 2020 in Reproductive Medicine Center in our hospital was conducted. According to whether HBsAg was seropositive or not，the patients were divided into group A（male partners being HBV+）；group B （female partners being HBV+）；group C（both partners being HBV+）and group D（controls，both partners being HBV⁃seronegative）. The differences of basic parameters，clinical pregnancy and neonatal outcomes among the four groups were compared. Results Compared with that in the control group，there was no difference in pregnancy outcomes in group A，B and C. In terms of the neonatal outcomes，we found the cesarean rate was significantly higher in group A when compared with the control group（67.3% vs. 46.7%，P = 0.010）. Moreover，the sex ratio of offspring in group A was statistically higher than that in the control group［（39∶14）vs.（107∶89），P = 0.013］. However，there was no difference between group B or C and the control group. All offspring were no deforming and free of HBV infection. Conclusion Different HBV infection patterns of couples have no effect on pregnancy out⁃ comes. However，male partners being HBV infection may increase the risk of cesarean rate，and has certain effect on the sex ratio of newborns.