Objective To investigate the relationship between susceptibility to allergic reaction induced by common allergens and HLA DRB1*0901（RS2395185）gene polymorphism in peripheral blood of patients with allergic skin disease. Methods A total of 304 patients who accepted treatment in department of dermatology in Guangzhou were selected. Nineteen kinds of allergens were detected by Western blotting. HLA DRB1 * 0901 geno⁃ types were detected by PCR sequencing. The relationship between HLA DRB1 * 0901 gene polymorphism and the risk of allergic reaction induced by allergens was analyzed. Results The G/G and G/T genotypes of rs2395185 were significantly different between the positive and negative patients（P < 0.05），and might be associated with the risk of house dust mite allergy（OR＝2.940，95%CI：1.167～7.407；OR＝2.889，95%CI：1.136～7.348）. There was significant difference in the distribution of G/T genotype between mild and moderate to severe allergic patients （P < 0.05）. G/T genotype may be a risk factor for the severity of allergic reaction（OR＝8.681，95%CI：1.077~ 69.989）. Conclusion The risk of skin allergy caused by Dermatophagoides pteronyssinus in population of Guang⁃ zhou may be related to the G/G and G/T polymorphisms of HLA DRB1 * 09：01（rs2395185），and the severity of allergy to 19 allergens may be related to the G/T polymorphism of rs2395185.