Objective To investigatethe effect and mechanism of anal lotion on repairing wounds inanal fistula model rats. Methods Forty rats were randomly divided into the normal control group，model group，anal lotion group and positive control group，with 10 rats in each group. After the 14⁃day experiment，the wound healing rate was calculated by taking pictures of the wound with a fixed focal length，and angiopoietin⁃I（Ang⁃I），angiopoi⁃ etin⁃Ⅱ（Ang⁃Ⅱ），vascular endothelial growth factor（VEGF），prostaglandin E2（PGE2），interleukin⁃2（IL⁃2） and interleukin ⁃ 1β（IL ⁃ 1β）were detected by enzyme ⁃linked immunoassay（ELISA）. The pathological changes were detected by HE staining. MRNA expression of AMPK，mTOR and Aktwasdetected by RT⁃PCR. Protein expres⁃ sion of AMPK，mTOR and Aktwasdetected by Western blot assay. Results Anal lotion could significantly im⁃ prove the wound healing ratein anal fistula rats，decreased the expression of PGE2，IL⁃1β andIL⁃2，increased the expression of Ang⁃Ⅰ，Ang⁃Ⅱand VEGF，as well as promoting the mRNA and protein expression of AMPK，mTOR andAkt. HE staining results showed that the neonatal epidermal creeping，capillary neovascularization，dense and orderly arrangement of collagen fibers，neonatal hair follicles and other more complete or maturing skin appendages were seen in the wounds of rats in the anal lotion group. Conclusion Anal lotion can inhibit the inflammatory reaction of anal fistula in rats，promoting angiogenesis and wound healing，which may be achieved by regulating the AMPK/mTOR/Aktsignal pathway.