The Journal of Practical Medicine >

2026 , Vol. 42 >Issue 14: 2533 - 2541

DOI: https://doi.org/10.3969/j.issn.1006-5725.2026.14.006

Oncology: Diagnosis, Treatment and Prevention

Effect of NEDD4L on proliferation and apoptosis of acute myeloid leukemia cells via the Wnt/β-catenin pathway

  • Yuancheng LIU ,
  • Chenglong HU ,
  • Wu ZHOU ,
  • Jingxin ZHANG ,
  • Qinglin ZHANG ,
  • Huali HU ,
  • Sixi WEI
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  • 1.School of Medical Laboratory Science，Guizhou Medical University，Guiyang 550004，Guizhou，China
    2.Clinical Laboratory Center，the Affiliated Hospital of Guizhou Medical University，Guiyang 550004，Guizhou，China

Received date: 2026-03-24

  Online published: 2026-08-05

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Abstract

Objective To investigate the effect of NEDD4L on the proliferation and apoptosis of acute myeloid leukemia （AML） cells and its underlying mechanism via the regulation of the Wnt/β-catenin pathway. Methods Stable NEDD4L-overexpressing and NEDD4L-knockdown Kasumi-1 and U937 cell lines were successfully established via lentiviral transduction technology. The efficiency of NEDD4L overexpression and knockdown was rigorously verified by RT-qPCR and Western blot. Cell proliferation was accurately assessed using the CCK-8 assay， and apoptosis was precisely detected through flow cytometry. The expression levels of the apoptosis-related proteins BAX and Bcl2， along with the key proteins of the Wnt/β-catenin pathway （β-catenin， c-Myc， and cyclin D1）， were measured by Western blot. Results Results from RT-qPCR and Western blot assays demonstrated that NEDD4L was successfully overexpressed and knocked down in Kasumi-1 and U937 cell lines （P < 0.05）. CCK-8 assay results indicated that overexpression of NEDD4L significantly inhibited the proliferative capacity of Kasumi-1 and U937 cells （P < 0.05）. Flow cytometry results showed that overexpression of NEDD4L significantly increased the apoptosis rate in Kasumi-1 and U937 cells. Western blot analysis results revealed that overexpression of NEDD4L upregulated the pro-apoptotic protein BAX and downregulated the anti-apoptotic protein Bcl2 （P < 0.05）. Mechanistic studies results showed that overexpression of NEDD4L decreased the expression of β-catenin， c-Myc， and cyclin D1， whereas knockdown of NEDD4L upregulated these proteins. Furthermore， the Wnt/β-catenin pathway agonist SKL2001 partially reversed the pathway inhibition induced by overexpression of NEDD4L （P < 0.05）. Conclusion NEDD4L inhibits the proliferation of AML cells and induces the apoptosis of AML cells by suppressing the activity of the Wnt/β-catenin pathway， which suggests that NEDD4L may serve as a potential therapeutic target for AML.

Key words： NEDD4L; Wnt/β-catenin pathway; acute myeloid meukemia; cell proliferation; cell apoptosis

Cite this article

Yuancheng LIU , Chenglong HU , Wu ZHOU , Jingxin ZHANG , Qinglin ZHANG , Huali HU , Sixi WEI . Effect of NEDD4L on proliferation and apoptosis of acute myeloid leukemia cells via the Wnt/β-catenin pathway[J]. The Journal of Practical Medicine, 2026 , 42(14) : 2533 -2541 . DOI: 10.3969/j.issn.1006-5725.2026.14.006

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