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The diagnostic value and clinical research of 5′⁃tRF⁃GlyGCC in the early stage of colorectal cancer
Received date: 2025-02-27
Online published: 2025-06-19
Objective To detect the content of 5'-tRF-GlyGCC in peripheral blood and explore its application value in the early diagnosis of colorectal cancer （CRC）. Methods Peripheral blood samples were randomly collected from 99 CRC patients， 11 patients with enteritis， 17 patients with colorectal polyps and 99 healthy individuals in the same period at the Affiliated Hospital of Guizhou Medical University. The content of 5'-tRF-GlyGCC in total RNA of peripheral blood was detected by fluorescence RT-PCR. The receiver operating characteristic curve （ROC） was established to analyze the value of 5'-tRF-GlyGCCcontent in the early diagnosis of CRC and to explore the relationship between its content and the clinical and pathological characteristics of CRC patients. Results The content of 5'-tRF-GlyGCC in the peripheral blood of CRC patients and colorectal polyp patients was significantly higher than that of healthy individuals and patients with enteritis， and the differences were statistically significant （P < 0.05）. The area under the curve （AUC） of 5'-tRF-GlyGCC for diagnosing CRC was 0.817 （95%CI： 0.763 ~ 0.871）， with a diagnostic sensitivity of 56.7% and a specificity of 99.9%. The content of 5'-tRF-GlyGCC in peripheral blood was significantly positively correlated with the levels of CEA and CA19-9 in CRC patients. Conclusion The detection of 5'-tRF-GlyGCC in peripheral blood has a high application value in the early diagnosis of CRC and can be used for clinical reference and research.
Long WANG , Yingmin WU , Sha SHANG , Lu WANG . The diagnostic value and clinical research of 5′⁃tRF⁃GlyGCC in the early stage of colorectal cancer[J]. The Journal of Practical Medicine, 2025 , 41(11) : 1730 -1735 . DOI: 10.3969/j.issn.1006-5725.2025.11.018
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