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Study on the improvement of cardiac and renal function in rats with cardiorenal syndrome by inhibiting fibrosis with 6-gingerol
Received date: 2025-03-28
Online published: 2025-06-19
Objective To evaluate whether 6-gingerol（6G）can inhibit fibrosis and improve the cardiac and renal function and in rats with cardiorenal syndrome. Methods In the in vitro experiments of this study， the incorporation of isotope-labeled amino acids was used to detect the intervention of 6-gingerol on normal rat kidney-49F （NRK-49F） and normal rat kidney-52E （NRK-52E） cells. 68 male SD rats weighing 200 ~ 250 g were used to establish a rat model of cardiorenal syndrome by ligating the left anterior descending coronary artery and performing 5/6 nephrectomy. The rats were randomly divided into a control group， a model group， a low dose 6-gingerol group （6 mg/kg）， a high dose 6-gingerol group （30 mg/kg）， and a losartan potassium group （20 mg/kg）. The 6-gingerol group received intraperitoneal injection of 6-gingerol， while the control group and model group received intraperitoneal injection of an equal amount of physiological saline. The losartan group received oral administration of losartan potassium for a total of 6 weeks. After successful modeling， blood samples were taken for biochemical and cardiac ultrasound examinations. After the experiment， blood， heart， and kidney samples were taken for Masson， immunohistochemistry， and Western blot. Results 6-gingerol 20 μmol/L can reduce NRK-49F collagen synthesis and inhibit NRK-52E protein synthesis. Biochemical results showed that the serum creatinine， urea nitrogen， and brain natriuretic peptide （BNP） levels of rats in the low and high dose 6-gingerol groups and the losartan group were all reduced， with high dose 6-gingerol groups and losartan group showing the most significant decrease （P < 0.05）. Echocardiographic parameters showed that the 6-gingerol group and losartan potassium group improved cardiac contractile function and ventricular remodeling in rats （P < 0.05）. Masson staining and Western Blot showed renal collagen deposition， with reduced expression of collagen I and α-SMA （P < 0.05）. Immunofluorescence showed a decrease in the expression of renal collagen deposition I， α-SMA， and TGF-β1 （P < 0.05）. Conclusion 6-Gingerol may improve the cardiac and renal function and renal fibrosis in rats with cardiorenal syndrome.
Key words： 6?gingerol; cardiorenal syndrome; fibrosis; cardiac function; renal function
Ting DENG , Qiang FU , Zhiliang LI , Qingmin CHU , Xiaoxiong. ZHOU . Study on the improvement of cardiac and renal function in rats with cardiorenal syndrome by inhibiting fibrosis with 6-gingerol[J]. The Journal of Practical Medicine, 2025 , 41(11) : 1627 -1636 . DOI: 10.3969/j.issn.1006-5725.2025.11.004
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