The Journal of Practical Medicine >

2025 , Vol. 41 >Issue 10: 1487 - 1495

DOI: https://doi.org/10.3969/j.issn.1006-5725.2025.10.009

Basic Research

Mechanism of Jisuishang Formula in cervical spondylotic myelopathy： Regulation of neuronal injury via the Wnt/β⁃catenin signaling pathway

  • Hanli YANG ,
  • Chunzhi LIU ,
  • Ming SHI ,
  • Minggao HU ,
  • Xianzhong BU ,
  • Yuanming ZHONG ,
  • Wei. XU
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  • *.The Graduate School of Guangxi University of Chinese Medicine，Nanning 530001，Guangxi，China

Received date: 2025-02-13

  Online published: 2025-05-21

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Abstract

Objective To investigate the mechanism of Jisuishang Formula on cervical myelopathy based on Wnt/β-catenin signaling pathway. Methods Thirty-six adult male SD rats were randomly divided into sham operation group， model group， positive control group （TAK-715， 50 mg/kg）， Jisuishang Formula low （9.7 g/kg）， medium （19.4 g/kg） and high （38.8 g/kg） dose groups， with 6 rats in each group for 4 weeks. The BBB score and inclined plate test were observed at 1， 2 and 4 weeks after surgery. HE and Nissl staining were used to observe the histopathology and neuronal condition of the spinal cord. Immunofluorescence was used to detect the protein expressions of BDNF， β-catenin， Bax and Bcl-2. Western blot and qRT-PCR were used to detect the expression of Wnt/β-catenin signaling pathway-related proteins and mRNAs. Results Compared with the sham group， the BBB score and inclined plate test score were significantly decreased （P < 0.05）， the expressions of BDNF， β-catenin and Bcl-2 decreased （P < 0.05）， the expression of Bax increased （P < 0.05）， the expressions of β-catenin， LRP-6 and p-GSK-3βdecreased （P < 0.05）， and the expressions of Caspase-3 and Caspase-9 increased （P < 0.05）. Compared with the model group， the BBB score and inclined plate test score were significantly increased in the high-dose Jisuishang Formula group （P < 0.05）， the expressions of BDNF， β-catenin and Bcl-2 increased （P < 0.05）， the expression of Bax decreased （P < 0.05）， the expressions of β-catenin， LRP-6 and p-GSK-3βincreased （P < 0.05）， and the expressions of Caspase-3 and Caspase-9 decreased （P < 0.05）. Conclusion Jisuishang Formula prescription can inhibit neuronal apoptosis， improve spinal cord microenvironment， and promote neurological function recovery by activating the Wnt/β-catenin signaling pathway.

Key words： Wnt/β-catenin signaling pathway; Jisuishang Formula; cervical spondylosis; neurological recovery

Cite this article

Hanli YANG , Chunzhi LIU , Ming SHI , Minggao HU , Xianzhong BU , Yuanming ZHONG , Wei. XU . Mechanism of Jisuishang Formula in cervical spondylotic myelopathy： Regulation of neuronal injury via the Wnt/β⁃catenin signaling pathway[J]. The Journal of Practical Medicine, 2025 , 41(10) : 1487 -1495 . DOI: 10.3969/j.issn.1006-5725.2025.10.009

References

1 WILLIAMS J， D'AMORE P， REDLICH N， et al. Degenerative Cervical Myelopathy： Evaluation and Management［J］. Orthop Clin North Am， 2022， 53（4）：509-521. doi:10.1016/j.ocl.2022.05.007
2 HEJRATI N， MOGHADDAMJOU A， MARATHE N， et al. Degenerative Cervical Myelopathy： Towards a Personalized Approach［J］. Can J Neurol Sci， 2022， 49（6）：729-740. doi:10.1017/cjn.2021.214
3 贺朋飞， 李晋虎， 陈毅， 等. 采用零切迹融合器的ACDF手术预后模型构建与验证：一项基于颈椎矢状位参数的回顾性研究［J］. 临床神经外科杂志， 2024， 21（5）：546-552，557.
4 刘磊， 宋飞霏， 马超， 等. 脚趾站立试验在退行性脊髓型颈椎病临床诊断中应用的可行性分析［J］. 中国骨与关节损伤杂志， 2024， 39（1）：1-6.
5 BERLIN C， MARINO A C， MUMMANENI P V， et al. Determining the time frame of maximum clinical improvement in surgical decompression for cervical spondylotic myelopathy when stratified by preoperative myelopathy severity： A cervical Quality Outcomes Database study［J］. J Neurosurg Spine， 2022， 37（5）：758-766. doi:10.3171/2022.5.spine211425
6 杨勇， 陈仁军， 葛建岭， 等. 基于Wnt/β-catenin信号通路研究右美托咪定对七氟烷诱发认知功能损伤的保护作用［J］. 实用医学杂志， 2024， 40（15）：2063-2068.
7 EMON S T， USLU S， AYDINLAR E I， et al. Effects of Ozone on Spinal Cord Recovery via the Wnt/ &Beta；-Catenin Pathway Following Spinal Cord Injury in Rats［J］. Turk Neurosurg， 2017， 27（6）：946-951.
8 WANG Q， WANG L， BOTCHWAY B， et al. OTULIN Can Improve Spinal Cord Injury by the NF-κB and Wnt/β-Catenin Signaling Pathways［J］. Mol Neurobiol， 2024， 61（11）：8820-8830. doi:10.1007/s12035-024-04134-3
9 ZHAO Z， GAO K， SHAO W， et al. Protocatechuic aldehyde promotes the functional recovery of spinal cord injury by activating the Wnt/β-catenin signaling pathway［J］. J Spinal Cord Med， 2024， 47（5）：669-680. doi:10.1080/10790268.2023.2183329
10 刘春志， 钟妮， 张鼎， 等. 基于PERK信号通路调控ERS介导神经元凋亡探讨脊髓伤方对大鼠脊髓压迫神经元的保护作用及机制［J］. 时珍国医国药， 2024， 35（13）：2905-2911.
11 GAO K， NIU J， DANG X. Neuroprotection of netrin-1 on neurological recovery via Wnt/β-catenin signaling pathway after spinal cord injury［J］. Neuroreport， 2020， 31（7）：537-543. doi:10.1097/wnr.0000000000001441
12 ZHANG X， PENG B， MA Z， et al. Anterior Cervical Discectomy and Fusion for the Treatment of Pincer Cervical Spondylotic Myelopathy： A Single-Center Retrospective Study［J］. World Neurosurg， 2024， 191：e772-e778. doi:10.1016/j.wneu.2024.09.041
13 MOWFORTH O D， DAVIES B M， KOTTER M R. Quality of Life Among Informal Caregivers of Patients With Degenerative Cervical Myelopathy： Cross-Sectional Questionnaire Study［J］. Interact J Med Res， 2019， 8（4）：e12381. doi:10.2196/12381
14 AGARWAL N， JOHNSON S E， BYDON M， et al. Cervical spondylotic myelopathy and driving abilities： Defining the prevalence and long-term postoperative outcomes using the Quality Outcomes Database［J］. J Neurosurg Spine， 2024， 40（5）：630-641. doi:10.3171/2023.11.spine23738
15 钟远鸣， 许伟， 李智斐， 等. 运用脊髓伤方治疗脊髓型颈椎病的临床研究［J］. 辽宁中医杂志， 2018， 45（10）：2133-2136.
16 钟远鸣， 张家立， 米琨， 等. 脊髓伤方治疗胸腰椎骨折并截瘫的疗效观察［J］. 辽宁中医杂志， 2004，31（1）：34-35.
17 GAO J， LIAO Y， QIU M， et al. Wnt/β-Catenin Signaling in Neural Stem Cell Homeostasis and Neurological Diseases［J］. Neuroscientist， 2021， 27（1）：58-72. doi:10.1177/1073858420914509
18 ZHANG L， YAN R， ZHANG Q， et al. Survivin， a key component of the Wnt/β-catenin signaling pathway， contributes to traumatic brain injury-induced adult neurogenesis in the mouse dentate gyrus［J］. Int J Mol Med， 2013， 32（4）：867-875. doi:10.3892/ijmm.2013.1456
19 CLEVERS H. Wnt/beta-catenin signaling in development and disease［J］. Cell， 2006， 127（3）：469-480. doi:10.1016/j.cell.2006.10.018
20 MACDONALD B T， TAMAI K， HE X. Wnt/beta-catenin signaling： components， mechanisms， and diseases［J］. Dev Cell， 2009， 17（1）：9-26. doi:10.1016/j.devcel.2009.06.016
21 NUSSE R， CLEVERS H. Wnt/β-Catenin Signaling， Disease， and Emerging Therapeutic Modalities［J］. Cell， 2017， 169（6）：985-999. doi:10.1016/j.cell.2017.05.016
22 CHENG J， SHEN W， JIN L， et al. Treadmill exercise promotes neurogenesis and myelin repair via upregulating Wnt/β?catenin signaling pathways in the juvenile brain following focal cerebral ischemia/reperfusion［J］. Int J Mol Med， 2020， 45（5）：1447-1463.
23 XU D， LI F， XUE G， et al. Effect of Wnt signaling pathway on neurogenesis after cerebral ischemia and its therapeutic potential［J］. Brain Res Bull， 2020， 164：1-13. doi:10.1016/j.brainresbull.2020.07.005
24 邹德峰， 杨晓松， 卜繁旺， 等. 蛇床子素激活wnt/β-catenin信号通路抑制急性脊髓损伤大鼠神经细胞凋亡［J］. 解剖科学进展， 2020， 26（3）：303-306.
25 XIANG Z， ZHANG S， YAO X， et al. Resveratrol promotes axonal regeneration after spinal cord injury through activating Wnt/ β-catenin signaling pathway［J］. Aging （Albany NY）， 2021， 13（20）：23603-23619. doi:10.18632/aging.203628
26 DING Y， CHEN Q. Recent advances on signaling pathways and their inhibitors in spinal cord injury［J］. Biomed Pharmacother， 2024， 176：116938. doi:10.1016/j.biopha.2024.116938
27 LI C， JIAO G， WU W， et al. Exosomes from Bone Marrow Mesenchymal Stem Cells Inhibit Neuronal Apoptosis and Promote Motor Function Recovery via the Wnt/β-catenin Signaling Pathway［J］. Cell Transplant， 2019， 28（11）：1373-1383. doi:10.1177/0963689719870999
28 莫怡， 卜若晨， 唐福波， 等. 基于网络药理学与分子对接技术探讨脊髓伤方治疗脊髓损伤的作用机制［J］. 海南医学， 2023， 34（11）：1521-1531.
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