The Journal of Practical Medicine >

2025 , Vol. 41 >Issue 8: 1130 - 1138

DOI: https://doi.org/10.3969/j.issn.1006-5725.2025.08.007

Basic Research

Effect of lactobacillus plantarum LB12 on renal calcium oxalate stones in rats

  • Jinshan YANG ,
  • Benzhong JIA ,
  • Siwen ZHONG ,
  • Tao LI ,
  • Dengbao. LI
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  • Clinical Medical College，Guizhou Medical University，Guiyang 550004，Guizhou，China；*Department of Urology，Affiliated Hospital of Guizhou Medical University，Guiyang 550001，Guizhou，China

Received date: 2024-12-18

  Online published: 2025-04-30

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Abstract

Objective To investigate the effect of Lactobacillus plantarum LB12 on kidney calcium oxalate stones in rats. Methods A total of 24 male SD rats aged 6 ~ 8 weeks were randomly divided into four groups： control group， model group， LB12 group， and LB12 intervention group， with 6 rats in each group. The control group and LB12 group had free access to water， and were gavaged with 2 mL of normal saline in the morning. In the evening， the control group received 2 mL of normal saline， while the LB12 group received 2 mL of Lactobacillus plantarum LB12 suspension. The model group and LB12 intervention group were given 1% ethylene glycol in drinking water and 2 mL of 2% ammonium chloride solution via gavage in the morning. In the evening， both groups received 2 mL of normal saline and 2 mL of Lactobacillus plantarum LB12 suspension， respectively. After the experiment， the body weight change trend of the rats was recorded， and the levels of urinary calcium， urinary oxalate， serum creatinine， blood urea nitrogen， renal SOD， and MDA were measured. Additionally， H&E staining， VK staining， immunohistochemistry， ROS fluorescence analysis， and Western blot （WB） were performed on kidney tissues. Results Lactobacillus plantarum LB12 demonstrated a 12.34% oxalate degradation rate in vitro. Further animal experiments indicated that Lactobacillus plantarum LB12 alleviated the weight loss trend in the model rats and reduced the levels of urinary calcium， urinary oxalate， serum creatinine， urea nitrogen， and MDA， while increasing SOD levels （P < 0.05）. Histopathological analysis revealed that the LB12 intervention group had significantly fewer renal crystals compared to the model group， with lower expressions of IL-6 and ROS （P < 0.01）. Western blot analysis demonstrated that Lactobacillus plantarum LB12 inhibited the expression of TLR4， NF-κB， and OPN proteins （P < 0.01）. Conclusions Lactobacillus plantarum LB12 can effectively alleviate the oxidative stress damage caused by calcium oxalate stones to the kidneys and reduce kidney crystal formation in rats. Furthermore， it inhibits the expression of TLR4/NF-κB and OPN， making it a potential therapeutic approach for the prevention and treatment of kidney calcium oxalate stones.

Key words： calcium oxalate; lactobacillus plantarum; oxidative stress; TLR4; OPN

Cite this article

Jinshan YANG , Benzhong JIA , Siwen ZHONG , Tao LI , Dengbao. LI . Effect of lactobacillus plantarum LB12 on renal calcium oxalate stones in rats[J]. The Journal of Practical Medicine, 2025 , 41(8) : 1130 -1138 . DOI: 10.3969/j.issn.1006-5725.2025.08.007

References

1 SOROKIN I， MAMOULAKIS C， MIYAZAWA K， et al. Epidemiology of stone disease across the world［J］. World J Urol， 2017， 35（9）： 1301-1320. doi:10.1007/s00345-017-2008-6
2 WALTER K. Kidney Stones［J］. JAMA， 2022， 328（9）： 898. doi:10.1001/jama.2022.12609
3 吕昂，范新，苏倩，余陈欢，等. 车前草醇提物治疗肾草酸钙结石的作用及其机制［J］. 中国临床药理学与治疗学， 2016， 21（11）： 1239-1245.
4 王演， 潘铁军， 刘振宇， 等. 肠道微生物网络在高草酸诱导大鼠肾损伤中的保护作用［J］. 实用医学杂志， 2024， 40（13）： 1771-1777.
5 ALFORD A， FURROW E， BOROFSKY M， et al. Animal models of naturally occurring stone disease［J］. Nat Rev Urol， 2020， 17（12）： 691-705. doi:10.1038/s41585-020-00387-4
6 SUN Y， SUN H， ZHANG Z， et al. New insight into oxidative stress and inflammatory responses to kidney stones： Potential therapeutic strategies with natural active ingredients［J］. Biomed Pharmacother， 2024， 179： 117333. doi:10.1016/j.biopha.2024.117333
7 WANG Z， ZHANG Y， ZHANG J， et al. Recent advances on the mechanisms of kidney stone formation （Review）［J］. Int J Mol Med， 2021， 48（2）： 149. doi:10.3892/ijmm.2021.4982
8 FALONY G. Beyond Oxalobacter： The gut microbiota and kidney stone formation［J］. Gut， 2018， 67（12）： 2078-2079. doi:10.1136/gutjnl-2018-316639
9 CAPOLONGO G， FERRARO P M， UNWIN R. Inflammation and kidney stones： Cause and effect？［J］. Curr Opin Urol， 2023， 33（2）： 129-135. doi:10.1097/mou.0000000000001066
10 YUAN T， XIA Y， LI B， et al. Gut microbiota in patients with kidney stones： A systematic review and meta-analysis［J］. BMC Microbiol， 2023， 23（1）： 143. doi:10.1186/s12866-023-02891-0
11 TIAN L， LIU Y， XU X， et al. Lactiplantibacillus plantarum J-15 reduced calcium oxalate kidney stones by regulating intestinal microbiota， metabolism， and inflammation in rats［J］. FASEB J， 2022， 36（6）： e22340. doi:10.1096/fj.202101972rr
12 XU M， QIN Y， XIA Y， et al. Screening of oxalate-degrading probiotics and preventive effect of Lactiplantibacillus plantarum AR1089 on kidney stones［J］. Food Funct， 2024， 15（19）： 10163-10178. doi:10.1039/d4fo03133d
13 纪媛媛， 马欣莹， 步雨珊， 等. 降解草酸盐益生菌的筛选及其体内功效评价［J］. 食品与发酵工业， 2022， 48（13）： 78-83.
14 WIGNER P， BIJAK M， SALUK-BIJAK J. Probiotics in the Prevention of the Calcium Oxalate Urolithiasis［J］. Cells， 2022， 11（2）： 284. doi:10.3390/cells11020284
15 WEI Z， CUI Y， TIAN L， et al. Probiotic Lactiplantibacillus plantarum N-1 could prevent ethylene glycol-induced kidney stones by regulating gut microbiota and enhancing intestinal barrier function［J］. FASEB J， 2021， 35（11）： e21937. doi:10.1096/fj.202100887rr
16 ZHU H， CAO C， WU Z， et al. The probiotic L. casei Zhang slows the progression of acute and chronic kidney disease［J］. Cell Metab， 2021， 33（10）： 1926-1942.e8. doi:10.1016/j.cmet.2021.06.014
17 WANG L， CHEN Z， LIU Y， et al. Ozone oxidative postconditioning inhibits oxidative stress and apoptosis in renal ischemia and reperfusion injury through inhibition of MAPK signaling pathway［J］. Drug Des Devel Ther， 2018， 12： 1293-1301. doi:10.2147/dddt.s164927
18 WIGNER P， GR?BOWSKI R， BIJAK M， et al. The Molecular Aspect of Nephrolithiasis Development［J］. Cells， 2021， 10（8）： 1926. doi:10.3390/cells10081926
19 PENG Q， LI C， ZHAO Y， et al. Protective Effect of Degraded Porphyra yezoensis Polysaccharides on the Oxidative Damage of Renal Epithelial Cells and on the Adhesion and Endocytosis of Nanocalcium Oxalate Crystals［J］. Oxid Med Cell Longev， 2021， 2021： 6463281. doi:10.1155/2021/6463281
20 WANG G， HAO M， LIU Q， et al. Protective effect of recombinant Lactobacillus plantarum against H（2）O（2）-induced oxidative stress in HUVEC cells［J］. J Zhejiang Univ Sci B， 2021， 22（5）： 348-365. doi:10.1631/jzus.2000441
21 JIANG K， SUN Y， CHEN X. Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury： A Narrative Overview of Preclinical Studies［J］. Front Pharmacol， 2022， 13： 875103. doi:10.3389/fphar.2022.875103
22 LIU Y， SUN Y， KANG J， et al. Role of ROS-Induced NLRP3 Inflammasome Activation in the Formation of Calcium Oxalate Nephrolithiasis［J］. Front Immunol， 2022， 13： 818625. doi:10.3389/fimmu.2022.818625
23 WANG Q， ZHANG J， CHEN X， et al. Renal tubular epithelial cells treated with calcium oxalate up-regulate S100A8 and S100A9 expression in M1-polarized macrophages via interleukin 6［J］. Iran J Basic Med Sci， 2023， 26（5）： 603-608.
24 LIU Y， YANG J， WENG D， et al. A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation （Ser536） in Renal Carcinoma Cells［J］. Int J Mol Sci， 2024， 25（7）： 3576. doi:10.3390/ijms25073576
25 JIA Q， HUANG Z， WANG G， et al. Osteopontin： An important protein in the formation of kidney stones［J］. Front Pharmacol， 2022， 13： 1036423. doi:10.3389/fphar.2022.1036423
26 TAGUCHI K， OKADA A， UNNO R， et al. Macrophage Function in Calcium Oxalate Kidney Stone Formation： A Systematic Review of Literature［J］. Front Immunol， 2021， 12： 673690. doi:10.3389/fimmu.2021.673690
27 KHAN S R， CANALES B K， DOMINGUEZ-GUTIERREZ P R. Randall's plaque and calcium oxalate stone formation： Role for immunity and inflammation［J］. Nat Rev Nephrol， 2021， 17（6）： 417-433. doi:10.1038/s41581-020-00392-1
28 MEHRA Y， RAJESH N G， VISWANATHAN P. Analysis and Characterization of Lactobacillus paragasseri and Lacticaseibacillus paracasei： Two Probiotic Bacteria that Can Degrade Intestinal Oxalate in Hyperoxaluric Rats［J］. Probiotics Antimicrob Proteins， 2022， 14（5）： 854-872. doi:10.1007/s12602-022-09958-w
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