The Journal of Practical Medicine >

2025 , Vol. 41 >Issue 2: 186 - 194

DOI: https://doi.org/10.3969/j.issn.1006-5725.2025.02.005

Basic Research

Effect of miR⁃378⁃5p on angiogenesis of glioma

  • Jiayi CAI ,
  • Siyu CHEN ,
  • Nyulue CAI ,
  • Wende. LI
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  • 1.Animals Department of Guangdong Laboratory Animals Monitoring Institute，Guangzhou 510663，Guangdong，China
    2.Pharmacy Department of the First Affiliated Hospital of Zhejiang Chinese Medical University，Hangzhou 310006，Zhejiang，China
    3.Clinical Pharmacy Teaching and Research Office of Zhejiang Chinese Medical University，Hangzhou 310006，Zhejiang，China

Received date: 2024-10-10

  Online published: 2025-01-26

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Abstract

Objective To investigate the effect of miR-378-5p on glioma angiogenesis and its molecular mechanism. Methods The expression of miR-378-5p were detected by qRT-PCR in four glioma cells. The levels of VEGF were analyzed by ELISA. HUVECs were treated conditioned medium which collected by glioma cells supernatant. The effects of miR-378-5p on the proliferation， migration， invasion， and angiogenesis of HUVECs were detected by CCK-8， Transwell assay， and tubular formation assay. Results U251 was the highest expression of miR-378-5p， while U87 MG was the lowest expression. Glioma-conditioned media increased miR-378-5p levels significantly promoted the proliferation， migration， invasion， and angiogenesis of HUVECs. The results were contrary with glioma-conditioned media decreased miR-378-5p levels. miR-378-5p promoted the release of VEGF-A from glioma cells. The proliferation， migration， invasion， and angiogenesis of HUVECs treated-VEGF neutralizing antibody were significantly downregulated. Conclusion miR-378-5p promoted HUVECs proliferation， migration， invasion， and angiogenesis by increasing the release of VEGF from glioma cells.

Key words： microRNA-378-5p; glioma; angiogenesis; VEGF-A

Cite this article

Jiayi CAI , Siyu CHEN , Nyulue CAI , Wende. LI . Effect of miR⁃378⁃5p on angiogenesis of glioma[J]. The Journal of Practical Medicine, 2025 , 41(2) : 186 -194 . DOI: 10.3969/j.issn.1006-5725.2025.02.005

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