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Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia⁃reperfusion injury
Received date: 2024-01-15
Online published: 2024-10-22
Objective To analyze the effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia?reperfusion injury. Methods 50 SPF grade Kunming mice， with 10 as the normal group，38 mices were successfully modeled and divided into a model group of 9， a mitochondrial transplantation group of 9， an Agatroban group of 10， and a combination group of 10. The mitochondrial transplantation group was given exogenous mitochondrial extract， the agatroban group was given agatroban， the combined group was given agatroban combined with mitochondrial transplantation， and the blank group and model group were injected with normal saline. Observation of changes in cerebral infarction volume， pathological morphology of brain tissue， endothelial function， hemorheology， and relative expression of apoptosis related proteins in mice after 7 days of intervention. Results Compared with normal group， cerebral infarction volume ratio， ET?1， whole blood viscosity， plasma viscosity， Caspase-3 and Bax protein expression levels were increased in model group， mitochondrial transplantation group， agatroban group and combined group， while NO level and Bcl-2 protein expression levels were decreased （P < 0.05）. Compared with the model group， the cerebral infarction volume ratio， ET-1， whole blood viscosity， plasma viscosity， Caspase-3 and Bax protein expressions in the mitochondrial transplantation group， agatroban group and combined group were decreased， and the combined group was lower than the mitochondrial transplantation group and agatroban group， and the NO level and Bcl-2 protein expression were increased. The combined group was higher than that of mitochondrial transplantation group and agatriban group （P < 0.05）. Conclusion In mice with cerebral ischemia-reperfusion injury， endothelial function injury was improved， hemorheology was regulated， and brain injury was alleviated after agatroban combined with mitochondrial transplantation.
Yingjun TAO , Tengzhu REN , Feng WEI , Xintong. LIU . Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia⁃reperfusion injury[J]. The Journal of Practical Medicine, 2024 , 40(19) : 2665 -2671 . DOI: 10.3969/j.issn.1006-5725.2024.19.001
| 1 | LIU Y， ZHU X， TONG X， et al. Syringin protects against cerebral ischemia/reperfusion injury via inhibiting neuroinflammation and TLR4 signaling［J］. Perfusion， 2022，37（6）：562-569. doi:10.1177/02676591211007025 |
| 2 | GONG S， CAO G， LI F， et al. Endothelial Conditional Knockdown of NMMHC IIA （Nonmuscle Myosin Heavy Chain IIA） Attenuates Blood-Brain Barrier Damage During Ischemia-Reperfusion Injury［J］. Stroke， 2021，52（3）：1053-1064. doi:10.1161/strokeaha.120.031410 |
| 3 | ZHENG K， LIN L， JIANG W， et al. Single-cell RNA-seq reveals the transcriptional landscape in ischemic stroke［J］. J Cereb Blood Flow Metab， 2022，42（1）：56-73. doi:10.1177/0271678x211026770 |
| 4 | MA C， WANG X， XU T， et al. Qingkailing injection ameliorates cerebral ischemia-reperfusion injury and modulates the AMPK/NLRP3 Inflammasome Signalling pathway［J］. BMC Complement Altern Med， 2019，19（1）：320. doi:10.1186/s12906-019-2703-5 |
| 5 | ANDRABI S S， PARVEZ S， TABASSUM H. Ischemic stroke and mitochondria： Mechanisms and targets［J］. Protoplasma， 2020，257（2）：335-343. doi:10.1007/s00709-019-01439-2 |
| 6 | CHEN H S， CUI Y， ZHOU Z H， et al. Effect of Argatroban Plus Intravenous Alteplase vs Intravenous Alteplase Alone on Neurologic Function in Patients With Acute Ischemic Stroke： The ARAIS Randomized Clinical Trial［J］. JAMA， 2023，329（8）：640-650. doi:10.1001/jama.2023.0550 |
| 7 | 应夏丽，王晓玲，陈妍月，等. 马钱苷对小鼠脑缺血再灌注损伤保护作用的实验研究［J］. 中国中医药科技，2021，28（6）：888-893. |
| 8 | 方雪，邵卫，许慧芳，等. 基于2?Cl?MGV?1/BDNF?TrkB通路探讨脑梗死后认知功能改善的研究［J］. 实用医学杂志，2023，39（7）：819-826. |
| 9 | DAVE K M， STOLZl D B， MANICKAM D S. Delivery of mitochondria-containing extracellular vesicles to the BBB for ischemic stroke therapy［J］. Expert Opin Drug Deliv， 2023，20（12）：1769-1788. doi:10.1080/17425247.2023.2279115 |
| 10 | IKEDA G， SANTOSO M R， TADA Y， et al. Mitochondria-Rich Extracellular Vesicles From Autologous Stem Cell-Derived Cardiomyocytes Restore Energetics of Ischemic Myocardium［J］. J Am Coll Cardiol， 2021，77（8）：1073-1088. doi:10.1016/j.jacc.2020.12.060 |
| 11 | ZHANG X， ZHONG W， XUE R， et al. Argatroban in Patients With Acute Ischemic Stroke With Early Neurological Deterioration： A Randomized Clinical Trial［J］. JAMA Neurol， 2024，81（2）：118-125. doi:10.1001/jamaneurol.2023.5093 |
| 12 | LV B， GUO F F， LIN J C， et al. Efficacy and safety of argatroban in treatment of acute ischemic stroke： A meta-analysis［J］. World J Clin Cases， 2022，10（2）：585-593. doi:10.12998/wjcc.v10.i2.585 |
| 13 | XU S， ZHANG W， ZHANG Y， et al. Efficacy and Prognosis of Adjuvant Argatroban Treatment in Acute Ischemic Stroke Patients with Early Neurological Deterioration［J］. Discov Med， 2023，35（175）：185-192. doi:10.24976/discov.med.202335175.19 |
| 14 | CHEN B， ZHANG Y， CHEN S， et al. The role of vascular endothelial growth factor in ischemic stroke［J］. Pharmazie， 2021，76（4）：127-131. |
| 15 | LI J， YANG R， WANG S， et al. Bioinformatics-based analysis of mechanistic differences in vascular endothelial injury ischemic stroke induced by atrial fibrillation and atherosclerosis［J］. J Neurochem，2023，166（2）：265-279. doi:10.1111/jnc.15849 |
| 16 | TSAO C C， BAUMANN J， HUANG S F， et al. Pericyte hypoxia-inducible factor-1 （HIF-1） drives blood-brain barrier disruption and impacts acute ischemic stroke outcome［J］. Angiogenesis， 2021，24（4）：823-842. doi:10.1007/s10456-021-09796-4 |
| 17 | DANIELE E， NAZER Y， KORTEBI I， et al. Oral probiotic therapy improves motor function in a rodent model of sensorimotor stroke［J］. Exp Brain Res， 2023，241（7）：1931-1943. doi:10.1007/s00221-023-06651-4 |
| 18 | ALRAFIAH A R. Secondary Cerebellar Cortex Injury in Albino Male Rats after MCAO： A Histological and Biochemical Study［J］. Biomedicines， 2021，9（9）：1267. doi:10.3390/biomedicines9091267 |
| 19 | 彭羽， 林赟， 余能伟， 等. 银杏叶提取物联合低频重复经颅磁刺激治疗缺血性脑卒中的临床研究［J］. 四川大学学报（医学版）， 2021， 52（5）： 883-889. doi:10.12182/20210960202 |
| 20 | WU L， SHI P， ZHAO Y， et al. Hemorheology and Inflammatory Marker Changes in Patients with Acute Ischemic Stroke after Intravenous Thrombolysis with Mechanical Thrombectomy［J］. Pak J Med Sci， 2024，40（3）：342-346. doi:10.12669/pjms.40.3.8396 |
| 21 | GYAWALI P， LILLICRAP TP， TOMARI S， et al. Whole blood viscosity is associated with baseline cerebral perfusion in acute ischemic stroke［J］. Neurol Sci， 2022，43（4）：2375-2381. doi:10.1007/s10072-021-05666-5 |
| 22 | MA L， CHEN W， PAN Y， et al. Comparison of VerifyNow， thromboelastography， and PL-12 in patients with minor ischemic stroke or transient ischemic attack［J］. Aging （Albany NY）， 2021，13（6）：8396-8407. doi:10.18632/aging.202650 |
| 23 | LUO D， YU B， SUN S， et al. Effects of adjuvant berberine therapy on acute ischemic stroke： A meta-Analysis［J］. Phytother Res， 2023，37（9）：3820-3838. doi:10.1002/ptr.7920 |
| 24 | TANI A， SAKAKIMA H， OTSUKA S， et al. Stimulation of functional recovery via neurorepair mechanisms by the traditional Japanese Kampo medicine， Ninjin'yoeito， and physical exercise in a rat ischemic stroke model［J］. J Ethnopharmacol， 2023，302（1）：115927. doi:10.1016/j.jep.2022.115927 |
| 25 | SHARMA H， REETA K H， SHARMA U， et al. AMPA receptor modulation through sequential treatment with perampanel and aniracetam mitigates post-stroke damage in experimental model of ischemic stroke［J］. Naunyn Schmiedebergs Arch Pharmacol， 2023，396（12）：3529-3545. doi:10.1007/s00210-023-02544-z |
| 26 | ZHENG Z， HOU F， HE G， et al. Carvedilol Reduces the Neuronal Apoptosis after Ischemic Stroke by Modulating Activator of Transcription 3 Expression in vitro［J］. Dev Neurosci， 2023，45（2）：94-104. doi:10.1159/000527484 |
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