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Electroacupuncture improves gastrointestinal dyskinesia in rats with functional dyspepsia via PLC/IP3 pathway
Received date: 2023-12-14
Online published: 2024-08-26
Objective This study aims to determine whether electroacupuncture regulates the phospholipase C （PLC）/inositol-1，4，5-trisphosphate （PLC/IP3） pathway in platelet-derived growth factor receptor α-positive （PDGFRα+） cells， thereby improving gastrointestinal motility disorders in functional dyspepsia （FD）. Methods 40 SD rats were randomly divided into blank group， model group， electroacupuncture group， U73122 （PLC inhibitor） group， and U73122+electroacupuncture group， with 8 rats in each group. Except for the blank group， all rats were subjected to multi-factor stress intervention to establish the FD model. After successful modeling， the U73122 group was given intraperitoneal injection of inhibitor， the electroacupuncture group was acupunctured at Zusanli and Taichong points， and the U73122+electroacupuncture group was injected with inhibitor 2 hours before acupuncture. Ten days later， gastrointestinal motility was tested； immunoblotting was used to detect the protein expression levels of PDGFRα， PLC， P-PLC， IP3； immunofluorescence was used to detect the average fluorescence density and co-localization expression of PDGFRα and PLC， IP3； electron microscope was used to observe the gap junction （GJ） situation in the gastric antrum area. Results After modeling， the gastrointestinal motility of rats was weakened， the protein expression levels of PDGFRα， PLC， and IP3 were significantly reduced， GJ widened， and cell morphology changed； compared with the model group， the gastrointestinal motility of rats in the electroacupuncture group， U73122 group， and U73122+electroacupuncture group was significantly improved， the expression levels of PDGFRα， PLC， P-PLC， IP3 increased， GJ was slightly tight， and cell morphology recovered； there was no significant difference in the expression levels of PDGFRα， PLC， P-PLC， IP3 in the gastric antrum of U73122 group and U73122 + electroacupuncture group； PDGFRα， PLC， and IP3 had fluorescence co-localization. Conclusion Electroacupuncture can improve gastrointestinal dyskinesia in FD rats by activating the PLC/IP3 pathway in PDGFRα+ cells.
Deqian YANG , Qi CHEN , Shuwen JIN , Paidi. XU . Electroacupuncture improves gastrointestinal dyskinesia in rats with functional dyspepsia via PLC/IP3 pathway[J]. The Journal of Practical Medicine, 2024 , 40(16) : 2284 -2290 . DOI: 10.3969/j.issn.1006-5725.2024.16.014
| 1 | FORD A C， MAHADEVA S， CARBONE M F， et al. Functional dyspepsia ［J］. Lancet， 2020， 396（10263）： 1689-1702. doi:10.1016/s0140-6736(20)30469-4 |
| 2 | 吕咪，张坤漓，史中斐，等. 结合罗马Ⅳ标准探讨功能性消化不良、肠易激综合征重叠的现状［J］. 实用医学杂志，2021，37（09）：1213-1216. doi:10.3969/j.issn.1006-5725.2021.09.024 |
| 3 | WANG X， LIU H， LI W， et al. Bibliometric analysis of functional dyspepsia research trends over the past 20 years ［J］. Front Public Health， 2022， 10： 1019110. doi:10.3389/fpubh.2022.1019110 |
| 4 | LAN L， ZENG F， LIU G J， et al. Acupuncture for functional dyspepsia ［J］. Cochrane Database Syst Rev， 2014， 10： CD008487. doi:10.1002/14651858.cd008487.pub2 |
| 5 | VANUYTSEL T， BERCIK P， BOECKXSTAENS G. Understanding neuroimmune interactions in disorders of gut-brain interaction： from functional to immune-mediated disorders ［J］. Gut， 2023， 72（4）： 787-798. doi:10.1136/gutjnl-2020-320633 |
| 6 | HAN Y L， PENG X M， ZHANG H X， et al. Electroacupuncture Regulates TRPV1 through PAR2/PKC Pathway to Alleviate Visceral Hypersensitivity in FD Rats ［J］. Evid Based Complement Alternat Med， 2021， 2021： 1975228. doi:10.1155/2021/1975228 |
| 7 | ZHOU L， ZENG Y， ZHANG H， et al. The Role of Gastrointestinal Microbiota in Functional Dyspepsia： A Review ［J］. Front Physiol， 2022， 13： 910568. doi:10.3389/fphys.2022.910568 |
| 8 | OKATA T， ASANUMA K， NAKAGAWA K， et al. The Impact of Duodenal Mucosal Vulnerability in the Development of Epigastric Pain Syndrome in Functional Dyspepsia ［J］. Int J Mol Sci， 2022， 23（22）： 13947. doi:10.3390/ijms232213947 |
| 9 | CHENG J， GUO J， XU L， et al. The Overlap Subgroup of Functional Dyspepsia Exhibits More Severely Impaired Gastric and Autonomic Functions ［J］. J Clin Gastroenterol， 2024， 58（1）： 31-38. doi:10.1097/mcg.0000000000001802 |
| 10 | IINO S， HORIGUCHI K， HORIGUCHI S， et al. c-Kit-negative fibroblast-like cells express platelet-derived growth factor receptor alpha in the murine gastrointestinal musculature ［J］. Histochem Cell Biol， 2009， 131（6）： 691-702. doi:10.1007/s00418-009-0580-6 |
| 11 | LIN Q， QIN M， ZHAO S G， et al. The roles of PDGFRα signaling in the postnatal development and functional maintenance of the SMC-ICC-PDGFRα+ cell （SIP） syncytium in the colon ［J］. Neurogastroenterol Motil， 2019， 31（5）： e13568. doi:10.1111/nmo.13568 |
| 12 | 占煜，闻永，杜丽娟，等. 橙皮苷改善洛哌丁胺诱导便秘大鼠结肠SIP合胞体功能的研究［J］. 中国中西医结合杂志，2023，43（1）：67-75. |
| 13 | SANDERS K M， BAKER S A， DRUMM B T， et al. Ca（2+） Signaling Is the Basis for Pacemaker Activity and Neurotransduction in Interstitial Cells of the GI Tract ［J］. Adv Exp Med Biol， 2022， 1383： 229-241. doi:10.1007/978-3-031-05843-1_22 |
| 14 | FANG X， NI K， GUO J， et al. FRET Visualization of Cyclic Stretch-Activated ERK via Calcium Channels Mechanosensation While Not Integrin β1 in Airway Smooth Muscle Cells ［J］. Front Cell De Biol， 2022， 10： 847852. doi:10.3389/fcell.2022.847852 |
| 15 | MUSSA B M， KHAN A A， SRIVASTAVA A， et al. Differentiated PDGFRalpha-Positive Cells： A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase ［J］. Int J Mol Sci， 2021， 22（7）： 3514. doi:10.3390/ijms22073514 |
| 16 | 肖逸，周竟颖，尹鸿智，等. 电针“内关”“足三里”对功能性消化不良大鼠胃窦组织胃肠激素的影响［J］. 中国针灸， 2023， 43（12）： 1435-1440. |
| 17 | 潘小丽，周丽，王丹，等. 电针“足三里”对功能性消化不良大鼠胃排空反自噬信号通路的影响 ［J］. 针刺研究， 2019， 44（7）： 486-491. |
| 18 | 刘昊，王馨嫒，李文静，等. 病证结合的功能性消化不良动物模型的研究进展［J］. 中国实验动物学报，2023，31（10）：1342-1350. doi:10.3969/j.issn.1005-4847.2023.10.012 |
| 19 | HOU C， KIRCHNER T， SINGER M， et al. In vivo activity of a phospholipase C inhibitor， 1-（6-（（17beta-3-methoxyestra-1，3，5（10）-trien-17-yl）amino）hexyl）-1H-pyrrole-2，5-di one （U73122）， in acute and chronic inflammatory reactions ［J］. J Pharmacol Exp Ther， 2004， 309（2）： 697-704. doi:10.1124/jpet.103.060574 |
| 20 | CHEN S H， ZHU L J， ZHI Y H， et al. Pitongshu alleviates the adverse symptoms in rats with functional dyspepsia through regulating visceral hypersensitivity caused by 5-HT overexpression ［J］. Comb Chem High Throughput Screen， 2022， 26（7）： 1424-1436. doi:10.2174/1386207325666220827152654 |
| 21 | WAUTERS L， TALLEY N J， WALKER M M， et al. Novel concepts in the pathophysiology and treatment of functional dyspepsia ［J］. Gut， 2020， 69（3）： 591-600. doi:10.1136/gutjnl-2019-318536 |
| 22 | 金舒文，刘伟，刘嘉宝，等. 基于NGF/TrKA/TRPV1通路探讨电针改善功能性消化不良大鼠胃高敏感性［J］. 实用医学杂志，2023，39（22）：2928-2933. doi:10.3969/j.issn.1006-5725.2023.22.012 |
| 23 | VANDENBERGHE A， SCHOL J， VAN DEN HOUTE K， et al. Current and emerging therapeutic options for the management of functional dyspepsia ［J］. Expert opinion on pharmacotherapy， 2020， 21（3）： 365-376. doi:10.1080/14656566.2019.1707805 |
| 24 | ZHAO J， ZHAO L， ZHANG S， et al. Modified Liu-Jun-Zi decoction alleviates visceral hypersensitivity in functional dyspepsia by regulating EC cell-5HT3r signaling in duodenum ［J］. J Ethnopharmacol， 2020， 250： 112468. doi:10.1016/j.jep.2019.112468 |
| 25 | HOSIE S， ABO-SHABAN T， LEE C Y Q， et al. The Emerging Role of the Gut-Brain-Microbiota Axis in Neurodevelopmental Disorders ［J］. Adv Exp Med Biol， 2022， 1383： 141-156. doi:10.1007/978-3-031-05843-1_14 |
| 26 | VICENTINI F A， FAHLMAN T， RAPTIS S G， et al. New Concepts of the Interplay Between the Gut Microbiota and the Enteric Nervous System in the Control of Motility ［J］. Adv Exp Med Biol， 2022， 1383： 55-69. doi:10.1007/978-3-031-05843-1_6 |
| 27 | HWANG S J， DRUMM B T， KIM M K， et al. Calcium transients in intramuscular interstitial cells of Cajal of the murine gastric fundus and their regulation by neuroeffector transmission ［J］. J Physiol， 2022， 600（20）： 4439-4463. doi:10.1113/jp282876 |
| 28 | PIERRE O， FOUCHARD M， BUSCAGLIA P， et al. Calcium Increase and Substance P Release Induced by the Neurotoxin Brevetoxin-1 in Sensory Neurons： Involvement of PAR2 Activation through Both Cathepsin S and Canonical Signaling ［J］. Cells， 2020， 9（12）： 2704. doi:10.3390/cells9122704 |
| 29 | KIM H J. A functional role for the 'fibroblast-like cells' in gastrointestinal smooth muscles （J Physiol 2011；589［Pt 3］：697-710） ［J］. J Neurogastroenterol Motil， 2011， 17（4）： 425-426. doi:10.5056/jnm.2011.17.4.425 |
| 30 | GáRRIZ A， AUBRY S， WATTIAUX Q， et al. Role of the Phospholipase C Pathway and Calcium Mobilization in Oxytocin-Induced Contraction of Lacrimal Gland Myoepithelial Cells ［J］. Invest Opthalmol Vis Sci， 2021， 62（14）： 25. doi:10.1167/iovs.62.14.25 |
| 31 | BAKER S A， HWANG S J， BLAIR P J， et al. Ca（2+） transients in ICC-MY define the basis for the dominance of the corpus in gastric pacemaking ［J］. Cell Calcium， 2021， 99： 102472. doi:10.1016/j.ceca.2021.102472 |
| 32 | MARCHI S， GIORGI C， GALLUZZI L， et al. Ca2+ Fluxes and Cancer ［J］. Mol Cell， 2020， 78（6）： 1055-1069. doi:10.1016/j.molcel.2020.04.017 |
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