The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 16: 2263 - 2269

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.16.011

Basic Research

MiR⁃365a⁃3p affects vascular endothelial cell function through TGF⁃β signaling pathway and participates in the pathogenesis of preeclampsia

  • Zhaohua YAN ,
  • Jianbin ZHENG ,
  • Na ZHANG ,
  • Chunyan CAO ,
  • Luchun. YAN
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  • *.Department of Obstetrics and Gynecology，Affiliated Hospital of Guangdong Medical University，Zhanjiang 524001，China

Received date: 2023-07-03

  Online published: 2024-08-26

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Abstract

Objective To explore the mechanism of microRNA （miRNA）-365a-3p affecting the function of vascular endothelial cells involved in the pathogenesis of preeclampsia （PE）. Methods Primary human umbilical vein endothelial cells （HUVECs） were set as a NC group （transfected miR-365a-3p NC）， a mimics group （transfected miR-365a-3p mimics） and a inhibitor group （transfected miR-365a-3p inhibitor）. Logarithmic HUVECs cells were set as the blank group. The cell proliferation， migration and angiogenesis in each group were detected. Dual luciferin assay verified the targeting relationship between miR-365a-3p and downstream gene. The protein expressions of TGF-β1， Smad4 and Smad7 in each group were detected. Results Compared with the blank group and the NC group， the absorbance value and mobility of 24， 48 and 72 h were decreased （P < 0.05）， the number of tubular structures per field were decreased in the mimics group （P < 0.05）， the absorbance value and mobility of 24， 48 and 72 h were increased （P < 0.05）， and the number of tubular structures per field were increased in the inhibitor group （P < 0.05）. Dual luciferin assay showed that Smad7 was a target gene of miR-365a-3p. Compared with the blank group and the NC group， the protein expressions of TGF-β1 and Smad4 in the mimics group were increased （P < 0.05）， while the protein expression of Smad7 was decreased （P < 0.05）. The protein expression levels of TGF-β1 and Smad4 in the inhibitor group were decreased （P < 0.05）， while the protein expression levels of Smad7 were increased （P < 0.05）. Conclusion miR-365a-3p may affect the function of vascular endothelial cells by regulating the downstream TGF-β signaling pathway， and thus participate in the pathogenesis of PE.

Key words： preeclampsia; microRNA-365a-3p; vascular endothelial cell function; transforming growth factor beta

Cite this article

Zhaohua YAN , Jianbin ZHENG , Na ZHANG , Chunyan CAO , Luchun. YAN . MiR⁃365a⁃3p affects vascular endothelial cell function through TGF⁃β signaling pathway and participates in the pathogenesis of preeclampsia[J]. The Journal of Practical Medicine, 2024 , 40(16) : 2263 -2269 . DOI: 10.3969/j.issn.1006-5725.2024.16.011

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