The Journal of Practical Medicine >
The expression and clinical significance of hsa_circ_0003922 in endometrioid carcinoma
Received date: 2023-09-09
Online published: 2024-07-15
Objective Investigation of the expression and clinical significance of circular RNA hsa_circ_0003922 in endometrioid carcinoma （EEC）. Methods Three EEC tissues and three normal endometrial tissues were selected for high-throughput molecular sequencing to identify specific circRNAs and related functional molecules. Additionally， 36 EEC patients were selected as the experimental group， while 36 patients with benign uterine lesions served as the control group. The differentially expressed hsa_circ_0003922 was chosen as the research target， and its expression in EEC tissues and the control group was detected using real-time fluorescence quantitative PCR （RT-qPCR）. The relationship between its expression level and clinical pathological features of EEC was analyzed. Furthermore， the miRNAs associated with hsa_circ_0003922 were predicted. The receiver operating characteristic （ROC） curve analysis was employed to evaluate the diagnostic value of hsa_circ_0003922 for EEC. Results The results of high-throughput molecular sequencing showed that there were 293 differentially expressed circRNAs in EEC tissues compared to the control group， including 115 upregulated and 178 downregulated circRNAs. RT-qPCR further revealed that the expression of hsa_circ_0003922 was significantly decreased in EEC tissues compared to the control group （P < 0.05）. The expression level of hsa_circ_0003922 was significantly negatively correlated with FIGO staging and histological grading of EEC （P < 0.05）. The area under the ROC curve （AUC） for hsa_circ_0003922 in distinguishing EEC patients from the control group was 0.7724 （P<0.05）. Conclusion The expression of hsa_circ_0003922 is associated with FIGO staging and histological grading of EEC， indicating its potential as a reference index for auxiliary diagnosis， prognosis evaluation， and as a potential molecular target for adjuvant therapy in EEC.
Key words： endometrioid carcinoma; circular RNAs; biomarkers; expression; diagnosis
Jie YANG , Chunhong LI , Jingfei HUANG , Zhiwei CHEN , Lin. LIU . The expression and clinical significance of hsa_circ_0003922 in endometrioid carcinoma[J]. The Journal of Practical Medicine, 2024 , 40(14) : 1975 -1980 . DOI: 10.3969/j.issn.1006-5725.2024.14.013
| 1 | SUNG H， FERLAY J， SIEGEL R L， et al. Global Cancer Statistics 2020： GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries［J］. CA Cancer J Clin， 2021， 71（3）： 209-249. doi:10.3322/caac.21660 |
| 2 | 中国抗癌协会妇科肿瘤专业委员会， 中华医学会病理学分会， 国家病理质控中心. 子宫内膜癌分子检测中国专家共识（2021年版）［J］. 中国癌症杂志， 2021， 31（11）： 1126-1144. doi:10.19401/j.cnki.1007-3639.2021.11.012 |
| 3 | LAUBY-SECRETAN B， SCOCCIANTI C， LOOMIS D， et al. Body Fatness and Cancer--Viewpoint of the IARC Working Group［J］. N Engl J Med， 2016， 375（8）： 794-798. doi:10.1056/nejmsr1606602 |
| 4 | RAHIB L， SMITH B D， AIZENBERG R， et al. Projecting cancer incidence and deaths to 2030： the unexpected burden of thyroid， liver， and pancreas cancers in the United States［J］. Cancer Res， 2014， 74（11）： 2913-2921. doi:10.1158/0008-5472.can-14-0155 |
| 5 | 杜鲁涛， 靖旭， 段伟丽. 妇科肿瘤标志物应用专家共识［J］. 山东大学学报（医学版）， 2018， 56（10）： 3-8. |
| 6 | SIEGEL R L， MILLER K D， JEMAL A. Cancer statistics， 2018［J］. CA Cancer J Clin， 2018， 68（1）： 7-30. doi:10.3322/caac.21442 |
| 7 | HOLDT L M， KOHLMAIER A， TEUPSER D. Molecular roles and function of circular RNAs in eukaryotic cells［J］. Cellular & Molecular Life Sciences Cmls， 2018， 75（11）： 1071-1098. doi:10.1007/s00018-017-2688-5 |
| 8 | GUO J， TONG J， ZHENG J. Circular RNAs： A Promising Biomarker for Endometrial Cancer［J］. Cancer Manag Res， 2021， 13：1651-1665. doi:10.2147/cmar.s290975 |
| 9 | CHEN L L. The expanding regulatory mechanisms and cellular functions of circular RNAs［J］. Nat Rev Mol Cell Biol， 2020， 21（8）： 1-16. doi:10.1038/s41580-020-0243-y |
| 10 | 曹金秀， 江雁琼， 孙嘉. 环状RNA circ0023033及其靶基因在肺癌中的表达及诊断标志物意义［J］. 实用医学杂志， 2018， 34（14）： 2420-2424. doi:10.3969/j.issn.1006-5725.2018.14.034 |
| 11 | ZHANG S， XU Y， ZHENG Q. circRNA_0000285 knockdown suppresses viability and promotes apoptosis of cervical cancer cells by sponging microRNA-654-3p［J］. Bioengineered， 2022， 13（3）： 5251-5261. doi:10.1080/21655979.2022.2037870 |
| 12 | NI Y， LU C， WANG W， et al. circBANP promotes colorectal cancer growth and metastasis via sponging let-7d-5p to modulate HMGA1/Wnt/β-catenin signaling［J］. Mol Ther Oncolytics， 2021， 21： 119-133. doi:10.1016/j.omto.2021.03.012 |
| 13 | 何春萍， 黄超， 周瑞， 等. circLMNB1诱导调控内质网应激介导结直肠癌转移发生［J］. 实用医学杂志， 2021， 37（12）： 1645-1648. doi:10.3969/j.issn.1006 |
| 14 | YANG C， MOU Z， WU S， et al. High-throughput sequencing identified circular RNA circUBE2K mediating RhoA associated bladder cancer phenotype via regulation of miR-516b-5p/ARHGAP5 axis［J］. Cell Death Dis， 2021， 12（8）： 1-12. doi:10.1038/s41419-021-03977-1 |
| 15 | LIU L， SANG M， SHI J， et al. CircRNA mannosidase alpha class 1A member 2 promotes esophageal squamous cell carcinoma progression by regulating C-C chemokine ligand 5［J］. Biochem Biophys Res Commun， 2023， 645： 61-70. doi:10.1016/j.bbrc.2023.01.043 |
| 16 | 高丽娜， 董燕， 何晓春， 等. 环状RNA circAAS1对人绒毛膜滋养细胞侵袭及迁移的影响［J］. 实用医学杂志， 2022， 38（17）： 2151-2157. doi:10.3969/j.issn.1006-5725.2022.17.007 |
| 17 | LEWIS B P， SHIH I H， JONES-RHOADES M W， et al. Prediction of mammalian microRNA targets［J］. Cell， 2003， 115（7）： 787-798. doi:10.1016/s0092-8674(03)01018-3 |
| 18 | BETEL D， WILSON M， GABOW A， et al. The microRNA.org resource： targets and expression［J］. Nucleic Acids Res， 2008， 36（Database issue）： D149-D153. doi:10.1093/nar/gkm995 |
| 19 | QU S， YANG X， LI X， et al. Circular RNA： a new star of noncoding RNAs［J］. Cancer letters， 2015， 365（2）： 141-148. doi:10.1016/j.canlet.2015.06.003 |
| 20 | ZHOU Y， PAN A， ZHANG Y， et al. Hsa_circ_0039569 facilitates the progression of endometrial carcinoma by targeting the miR-197/high mobility group protein A1 axis［J］. Bioengineered， 2022， 13（2）： 4212-4225. doi:10.1080/21655979.2022.2027060 |
| 21 | MA J， ZHAO X， SHI L. Circ 003390/Eukaryotic translation initiation factor 4A3 promoted cell migration and proliferation in endometrial cancer via vascular endothelial growth factor signaling by miR-195-5p［J］. Bioengineered， 2022， 13（5）： 11958-11972. doi:10.1080/21655979.2022.2069358 |
| 22 | LIU Y， YUAN H， HE T. Downregulated circular RNA hsa_circ_0005797 inhibits endometrial cancer by modulating microRNA-298/Catenin delta 1 signaling［J］. Bioengineered， 2022， 13（3）： 4634-4645. doi:10.1080/21655979.2021.2013113 |
| 23 | WU X， HAN Y， LIU F， et al. Downregulations of miR-449a and miR-145-5p Act as Prognostic Biomarkers for Endometrial Cancer［J］. J Comput Biol， 2020， 27（5）： 834-844. doi:10.1089/cmb.2019.0215 |
| 24 | 门颖超，张蕾，艾浩. miR-145-5p靶向双特异性磷酸酶6对人子宫内膜癌增殖、迁移、侵袭与凋亡的影响［J］. 南方医科大学学报， 2020， 40（1）： 61-66. doi:10.12122/j.issn.1673-4254.2020.01.09 |
| 25 | SUN G， TIAN J， XIAO Y， et al. Circular RNA circ_0005667 promotes cisplatin resistance of endometrial carcinoma cells by regulating IGF2BP1 through miR-145-5p［J］. Anticancer Drugs， 2023， 34（7）： 816-826. doi:10.1097/cad.0000000000001479 |
/
| 〈 |
|
〉 |