Objective To explore the early prediction value of related test indicators for non-biliary acute pancreatitis and the influence of interaction of related test indicators on non-biliary acute pancreatitis. Methods A total of 280 patients with the first onset of non-biliary acute pancreatitis who were hospitalized in Jinshan Hospital of Fudan University from July 2019 to July 2023 were divided into mild group and severe group. The severe group included moderately severe and severe patients. Clinical data， tests and examination indicators of first-attack inpatients within 48 hours after admission were collected. Through logistics regression analysis， early detection indicators related to the severity of non-biliary acute pancreatitis were explored， and the influence of their interaction was studied. Results Single-factor analysis showed that albumin （ALB）， thyroxine （T4）， serum free triiodothyronine， albumin （ALB）， Thyroxine （T4） in both groups. FT3）， thyroid stimulating hormone （TSH）， serum triglyceride （TG）， calcium （Ca） and other indicators were statistically significant （P < 0.05）； Through multi-factor logistics regression analysis， it was found that the OR values of T4， FT3， TSH， CA and other detection indicators were all less than 1 （P < 0.05）； After controlling for gender， age， history of hypertension， history of diabetes and other factors， the interaction between TG and HbA1c was analyzed， the aOR was 1.500 when only HbA1c was increased （P > 0.05）， the aOR was 4.488 when only TG was increased （P < 0.05）， and the aOR was 5.084 when TG and HbA1c were increased simultaneously （P < 0.05）. Conclusion ALB， T4， FT3， TSH， CA were protective factors of non-biliary AP， and could be used as predictive indicators of the severity of non-biliary acute pancreatitis. Interaction of HbA1c with TG increases the risk of progression to severe non-biliary acute pancreatitis.