The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 12: 1631 - 1636

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.12.004

Basic Research

Effect of miR⁃15a⁃5p on autophagy of placental trophoblasts in preeclampsia

  • Sumei YU ,
  • Yuyue ZHANG ,
  • Liwen MA ,
  • Yuanjun KUANG ,
  • Qingning CHANG ,
  • Min KONG ,
  • ZHANG Huiping ZHANGx
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  • Clinical Medical College，Ningxia Medical University，Yinchuan 750004，China
    National Health Commission Key Laboratory of Metabolic Cardiovascular Disease Research，Yinchuan 750004，China
    General Hospital of Ningxia Medical University，Ningxia 750004，China

Received date: 2023-06-27

  Online published: 2024-06-14

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Abstract

Objective Investigating the impact of miR?15a?5p on autophagy in trophoblast cells of preeclamptic placenta. Methods Collect 20 cases of normal placental tissue and 20 cases of preeclamptic placental tissue from December 2020 to December 2022. Use fluorescence quantitative PCR to detect the expression of miR?15a?5p in placental tissue and trophoblast cells， and study its correlation with patient blood pressure. The HTR8?S/Vneo cells are divided into normal group （control） and hypoxia group， and the effect of hypoxia on the expression of miR?15a?5p is observed. Additionally， mimic?NC group， mimic?NC+hypoxia group， miR?15a?5p mimic group， miR?15a?5p mimic+hypoxia group， inhibitor?NC group， inhibitor?NC+hypoxia group， miR ?15a ?5p inhibitor group， and miR ?15a ?5p inhibitor +hypoxia groups are set up to observe the effect of miR ?15a ?5p on hypoxia?induced autophagy?related proteins LC3B and p62 protein in trophoblast cells. Western blot is used to detect the expression levels of autophagy?related proteins LC3B and p62 protein in each group； TargetScan website predicts the target genes of miR?15a?5p，and detects their expression levels in placental tissue and trophoblast cells. Results Compared with the control group， the expression levels of miR?15a?5p were significantly increased in the placentas and hypoxic trophoblasts of preeclampsia， and they were positively correlated with the blood pressure of the patients. Under hypoxic conditions， the overexpressed miR?15a?5p promoted the protein expression of LC3BII/I， while the relative expression of P62 was decreased. But after interference with miR?15a?5p， LC3BII/I expression was down?regulated and P62 expression was up?regulated. The results of quantitative PCR and Western blot showed that the expression levels of YAP1 in the preeclampsia placental tissues and hypoxic trophoblasts were significantly reduced. Conclusion The upregulation of miR?15a?5p in trophoblast cells of the placenta in individuals with preeclampsia could enhance autophagy in preeclampsia by forming a complex with YAP1.

Key words： preeclampsia; trophoblast; autophagy; miR?15a?5p; YAP1

Cite this article

Sumei YU , Yuyue ZHANG , Liwen MA , Yuanjun KUANG , Qingning CHANG , Min KONG , ZHANG Huiping ZHANGx . Effect of miR⁃15a⁃5p on autophagy of placental trophoblasts in preeclampsia[J]. The Journal of Practical Medicine, 2024 , 40(12) : 1631 -1636 . DOI: 10.3969/j.issn.1006-5725.2024.12.004

References

1 RANA S， LEMOINE E， GRANGER J P， et al. Preeclampsia［J］. Circ Res， 2019， 124（7）： 1094?1112. doi:10.1161/circresaha.118.313276
2 冷敏， 彭颖， 林晓莹， 等. 自噬在创面愈合中的作用研究进展［J］. 实用医学杂志， 2020， 36（22）： 3157?3165. doi:10.3969/j.issn.1006-5725.2020.22.026
3 冯晓玲， 姜天悦， 张杨. 胎盘自噬与子痫前期［J］.中国计划生育学杂志， 2022， 30（1）： 236?240.
4 KLIONSKY D J， PETRONI G， AMARAVADI R K， et al. Autophagy in major human diseases［J］. EMBO J， 2021， 40（19）：e108863.
5 CORNELIUS D C， WALLACE K. Autophagy in preeclampsia： A new target？［J］. EBioMedicine， 2020， 57：102864. doi:10.1016/j.ebiom.2020.102864
6 陈国庆， 陈璐， 曾发， 等. 子痫前期孕妇母胎界面炎症介质的表达与线粒体自噬的相关性研究［J］.国际妇产科学杂志， 2020， 47（3）： 271?275，361. doi:10.3969/j.issn.1674-1870.2020.03.007
7 CORREIA DE SOUSA M， GJORGJIEVA M， DOLICKA D， et al. Deciphering miRNAs' Action through miRNA Editing［J］. Int J Mol Sci， 2019， 20（24）：6249. doi:10.3390/ijms20246249
8 ESKANDARI F， REZAEI M， MOHAMMADPOUR?GHAREHBAGH A， et al. The association of pri?miRNA? 26a1 rs7372209 polymorphism and Preeclampsia susceptibility［J］. Clin Exp Hypertens， 2019， 41（3）： 268?273. doi:10.1080/10641963.2018.1469643
9 APICELLA C， RUANO C S M， MéHATS C， et al. The Role of Epigenetics in Placental Development and the Etiology of Preeclampsia［J］. Int J Mol Sci， 2019， 20（11）：2837. doi:10.3390/ijms20112837
10 LAGANà A S， VITALE S G， SAPIA F， et al. miRNA expression for early diagnosis of preeclampsia onset： hope or hype？［J］. J Matern Fetal Neonatal Med， 2018， 31（6）： 817?821. doi:10.1080/14767058.2017.1296426
11 LV Y， LU C， JI X， et al. Roles of microRNAs in preeclampsia［J］. J Cell Physiol， 2019， 234（2）： 1052?1061. doi:10.1002/jcp.27291
12 SKALIS G， KATSI V， MILIOU A， et al. MicroRNAs in Preeclampsia［J］. Microrna， 2019， 8（1）： 28?35. doi:10.2174/2211536607666180813123303
13 曾带娣， 李仲均， 萧丽娟， 等. 子痫前期患者胎盘中微小RNA?34a的表达及相关机制［J］. 发育医学电子杂志， 2020， 8（4）： 329?332.
14 潘耀平， 桂琦， 潘景. 自噬相关蛋白P62、OPTN在重度子痫前期胎盘滋养细胞中的表达及意义［J］.云南师范大学学报（自然科学版）， 2017， 37（3）： 63?65.
15 殷荷， 王艳华， 吴琪瑞， 等. miR?5088?5p在子痫前期患者胎盘组织中的表达及其对滋养细胞自噬的影响［J］.实用医学杂志， 2020， 36（24）： 3312?3316，3322.
16 朱亚飞， 张玉月， 谢琳， 等. 环状RNA circ＿BRAF＿2在子痫前期胎盘滋养细胞自噬中的作用［J］.中国临床药理学杂志， 2022， 38（23）： 2860?2864.
17 MICHALCZYK M， CELEWICZ A， CELEWICZ M， et al. The Role of Inflammation in the Pathogenesis of Preeclampsia［J］. Mediators Inflamm， 2020， 2020： 3864941. doi:10.1155/2020/3864941
18 ZHOU W， WANG H， YANG Y， et al. Trophoblast Cell Subtypes and Dysfunction in the Placenta of Individuals with Preeclampsia Revealed by Single?Cell RNA Sequencing［J］. Mol Cells， 2022， 45（5）： 317?328. doi:10.14348/molcells.2021.0211
19 GU S， ZHOU C， PEI J， et al. Esomeprazole inhibits hypoxia/endothelial dysfunction?induced autophagy in preeclampsia［J］. Cell Tissue Res， 2022， 388（1）： 181?194. doi:10.1007/s00441-022-03587-z
20 GLICK D， BARTH S， MACLEOD K F. Autophagy： cellular and molecular mechanisms［J］. J Pathol， 2010， 221（1）： 3?12. doi:10.1002/path.2697
21 IVES C W， SINKEY R， RAJAPREYAR I， et al. Preeclampsia?Pathophysiology and Clinical Presentations： JACC State?of?the?Art Review［J］. J Am Coll Cardiol， 2020， 76（14）： 1690?1702. doi:10.1016/j.jacc.2020.08.014
22 吴琪瑞， 王艳华， 柴丽芬， 等. circRNA RBM39与miR?5088?5p在缺氧诱导的滋养细胞自噬中的作用［J］.实用医学杂志， 2020， 36（5）： 570?574.
23 ZHANG H， HE Y， WANG J X， et al. miR?30?5p?mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia［J］. Redox Biol， 2020， 29： 101402. doi:10.1016/j.redox.2019.101402
24 PENG P， SONG H， XIE C， et al. miR?146a?5p?mediated suppression on trophoblast cell progression and epithelial?mesenchymal transition in preeclampsia［J］. Biol Res， 2021， 54（1）： 30. doi:10.1186/s40659-021-00351-5
25 ZHOU L， WANG W， WANG F， et al. Plasma?derived exosomal miR?15a?5p as a promising diagnostic biomarker for early detection of endometrial carcinoma［J］. Mol Cancer， 2021， 20（1）： 57. doi:10.1186/s12943-021-01352-4
26 KIM M， LY S H， XIE Y， et al. YAP1 and PRDM14 converge to promote cell survival and tumorigenesis［J］. Dev Cell， 2022， 57（2）： 212?227.e8. doi:10.1016/j.devcel.2021.12.006
27 顾天，刘春宏，张飞，等. 大黄素抑制胃癌AGS细胞YAP1、FOXD1基因表达及相关机制［J］. 实用医学杂志， 2024， 40（1）： 59?64. doi:10.3969/j.issn.1006-5725.2024.01.011
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