The Journal of Practical Medicine >
Effects of targeted temperature management based on different durations after traumatic brain injury in rats
Received date: 2023-07-18
Online published: 2024-06-14
Objective To investigate the protective effect of target temperature management based different durations with delayed hypothermia for traumatic brain injury（TBI） in rats. Methods 36 healthy adult male SD rats were randomly divided into NT group， HT4h group， HT24h group and HT48h group. The TBI model of rats was prepared with an electronic controllable cortical injury device. NT group was treated with normal temperature （37 ℃） 4 h after TBI， and the experimental groups were treated with low temperature （33.0 ± 1.0 ℃） 4 h after TBI for 4 h， 24 h， and 48 h. Three days after TBI， the motor function of the rats in each group was evaluated by beam walking test and inclined-grid climbing test， EB staining was used to measure the blood-brain barrier permeability，the change of hippocampal neurons was observed by Nissl staining， the expression of DCX and GFAP was detected by immunofluorescence， and the expression of Bcl-2 and Bax was measured by Western blot and immunohistochemistry. Results Compared with NT group， the experimental groups could significantly improve the motor function of TBI rats， reduce the permeability of blood-brain barrier，protect hippocampal neurons， promote DCX expression， inhibit GFAP expression， up-regulate the expression of Bcl-2 protein， and down-regulate the expression of Bax protein. However， the protective effect was more apparent in HT48h group than other experimental groups （P < 0.05）. Conclusion Long-term delayed target temperature management has a significant brain protective effect.
Wanyong ZHAO , Xiaopeng SUN , Jingjing WANG , Xiaohong LI , Hongtao SUN . Effects of targeted temperature management based on different durations after traumatic brain injury in rats[J]. The Journal of Practical Medicine, 2024 , 40(12) : 1625 -1630 . DOI: 10.3969/j.issn.1006-5725.2024.12.003
| 1 | CHRISTINE T， SUMAN R， ANDREW K， et al. Overview of Hypothermia， Its Role in Neuroprotection， and the Application of Prophylactic Hypothermia in Traumatic Brain Injury ［J］. Anesth Analg， 2023， 137（5）： 953-962. doi:10.1213/ane.0000000000006503 |
| 2 | YANG Z Y， SONG Z， HOU M. Target Temperature Management Versus Normal Temperature Management for Cardiac Arrest After Traumatic Brain Injury Patient： A Meta-Analysis and Systemic Review［J］. Ther Hypothermia Temp Manag， 2022， 12（3）：139-145. doi:10.1089/ther.2022.0007 |
| 3 | KWANG W. Target temperature management in traumatic brain injury with a focus on adverse events， recognition， and prevention ［J］. Acute Crit Care， 2022，37（4）：483-490. doi:10.4266/acc.2022.01291 |
| 4 | DIETRICH W D， BRAMLETT H M. Therapeutic hypothermia and targeted temperature management in traumatic brain injury： Clinical challenges for successful translation ［J］. Brain Res， 2016， 1640（Pt A）：94-103. doi:10.1016/j.brainres.2015.12.034 |
| 5 | SHOJI Y， HIROYUKI Y. Targeted temperature management in traumatic brain injury［J］. J Intensive Care， 2016， 4：28. doi:10.1186/s40560-016-0137-4 |
| 6 | GUIDO W， JOANNE O， SIMERDEEP K， et al. Therapeutic Hypothermia in Neonatal Hypoxic-Ischemic Encephalopathy［J］. Curr Neurol Neurosci Rep， 2019， 19（2）：2. doi:10.1007/s11910-019-0916-0 |
| 7 | HUI J F， FENG J F， TU Y， et al. Safety and efficacy of long-term mild hypothermia for severe traumatic brain injury with refractory intracranial hypertension （LTH-1）： a multicenter randomized controlled trial［J］. Eclinical Medicine， 2021， 32：100732. doi:10.1016/j.eclinm.2021.100732 |
| 8 | DAI H B， XU M M， LV J， et al. Mild Hypothermia Combined with Hydrogen Sulfide Treatment During Resuscitation Reduces Hippocampal Neuron Apoptosis Via NR2A， NR2B， and PI3K-Akt Signaling in a Rat Model of Cerebral Ischemia-Reperfusion Injury［J］. Mol Neurobiol， 2016， 53（7）： 4865-4873. doi:10.1007/s12035-015-9391-z |
| 9 | BAI Y N， YUAN M Y， MI H L， et al. Hypothermia reduces glymphatic transportation in traumatic edematous brain assessed by intrathecal dynamic contrast-enhanced MRI［J］. Front Neurol， 2022， 13：957055. doi:10.3389/fneur.2022.957055 |
| 10 | EIICHI S， HIROYASU K， HIROSUKE F， et al. Diverse effects of hypothermia therapy in patients with severe traumatic brain injury based on the computed tomography classification of the traumatic coma data bank［J］. J Neurotrauma， 2015， 32（5）：353-358. doi:10.1089/neu.2014.3584 |
| 11 | TURNER S， JOHN D， ZACHARY T， et al. Therapeutic hypothermia for intracerebral hemorrhage： Systematic review and meta-analysis of the experimental and clinical literature［J］. Int J Stroke， 2022， 17（5）：506-516. doi:10.1177/17474930211044870 |
| 12 | CUI H， YANG Z F， XIAO P， et al. Effects of Different Target Temperatures on Angiogenesis and Neurogenesis Following Resuscitation in a Porcine Model After Cardiac Arrest［J］. Shock， 2021， 55（1）：67-73. doi:10.1097/shk.0000000000001559 |
| 13 | 赵万勇， 李晓红， 王景景， 等. 基于不同时间窗的延迟亚低温对颅脑创伤大鼠Bcl-2、Bax和Caspase-3表达的影响［J］. 天津医药， 2017， 45（4）：364-367. |
| 14 | SUN D， RAYMOND J， WILSON P， et al. Cell proliferation and neuronal differentiation in the dentate gyrus in juvenile and adult rats following traumatic brain injury［J］. J Neurotrauma， 2005， 22（1）： 95-105. doi:10.1089/neu.2005.22.95 |
| 15 | 陈灿伟， 范子文， 黄帅， 等.香叶木素对前列腺癌PC-3细胞生物学行为的影响［J］. 实用医学杂志， 2022，38（6）：721-725. doi:10.3969/j.issn.1006-5725.2022.06.014 |
| 16 | JIN H， WEI L， GU X H， et al. Therapeutic effects of pharmacologically induced hypothermia against traumatic brain injury in mice［J］. J Neurotrauma， 2014， 31（16）：1417-1430. doi:10.1089/neu.2013.3251 |
| 17 | CHRISTOPHER M， ADAM F， CYNTHIA M， et al. Management of comfort and sedation in neonates with neonatal encephalopathy treated with therapeutic hypothermia［J］. Semin Fetal Neonatal Med， 2021， 26（4）：101264. doi:10.1016/j.siny.2021.101264 |
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