Objective To study EBV infection in nasopharyngeal carcinoma screening population in Guangzhou and analyze the influencing factors. Methods The data of individuals （aged 20 years and above） having EBV antibody of VCA-IgA and NA1-IgA test in Guangzhou Cadre and Talent Health Management Centerwere collected. Current status of EBV infection was studied and its influencing factors were analyzed by logistic regression analysis. Results A total of 55 311 subjects were screened， including 35 429 males （64.05%） and 19，882 females （35.95%）.The overall EBV infection rate was 8.00%. There was no significant differences in the infection rate between different sex groups（P > 0.05）. The infection rate of EBV increased with age， with infection rate of 7.07% in individuals aged 20 ~ 39 years， 7.86% in those aged 40 ~ 59 years，10.11 % in those of 60 ~ 79 years old， and 10.99% in those aged ≥ 80 years， showing statistical significance（χ² = 85.487，P < 0.01）. Logistic regression analysis showed that EBV infection risk increased significantly with advancing age. The odds of EBV infection in individuals aged 40 ~ 59 years were 10.6% higher compared to those aged 20 ~ 39 years （OR = 1.106， 95% CI： 1.028 ~ 1.190）. The odds of EBV infection in individuals aged 60 ~ 79 years were 42.2% higher compared to those aged 20 ~ 39 years （OR = 1.422， 95% CI： 1.288 ~ 1.569）. Furthermore， the odds of EBV infection in individuals aged ≥ 80 years were 53.1% higher compared to those aged 20 ~ 39 years （OR = 1.531， 95% CI： 1.230 ~ 1.906）.A prior diagnosis of hypertension significantly increased the odds of EBV infection （OR = 1.105， 95%CI： 1.003 ~1.218）. However， diabetes history exerted no influence on the outcome of EBV infection（P > 0.05）. Conclusions The overall infection rate of EBV in Guangzhou is 8.00%. Advanced age and hypertension history are the risk factors of EBV. The screening and prevention and control of EBV susceptible people should be strengthened.