The Journal of Practical Medicine >
Effect of Jinghou Zengzhi Granules on ovarian GDF9 secretion and granulosa cells apoptosis in controlled ovarian hyperstimulation rats
Received date: 2023-11-08
Online published: 2024-04-08
Objective To explore the effects of Jinghou Zengzhi Granules（JHZZG）， on ovarian GDF9 secretion and granulosa cells（GCs） apoptosis in controlled ovarian hyperstimulation（COH） rats through p38MAPK/CK2/IκBα/NF-κB pathway. Methods A model of COH rats was prepared and 18 rats were randomly divided into 3 groups， including natural ovulation group（NO group）， COH group， and COH+JHZZG group. The expression of p38MAPK， CK2， IκBα， NF-κB， GDF9 mRNA and protein were detected by qRT-PCR and Western blot， and the apoptosis rate of ovarian GCs by TUNEL. Results Compared with the NO group， the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH group increased， while the expression of CK2， IκBα and GDF9 decreased， and the apoptosis rate of ovarian GCs increased （all P < 0.01）. Compared with the COH group， the expression of p38MAPK and NF-κB in the ovarian tissue of rats in the COH+JHZZG group decreased， while the expression of CK2， IκBα and GDF9 increased （all P < 0.01）， and the apoptosis rate of ovarian GCs decreased （P < 0.05）. Conclusion Jinghou Zengzhi Granules could promote the secretion of ovarian GDF9 and inhibit the apoptosis of ovarian GCs of COH rats through p38MAPK/CK2/IκBα/NF-κB signaling pathway， which thereby improve the quality of COH oocytes.
Key words： Jinghou Zengzhi Granules; controlled ovarian hyperstimulation; p38MAPK; NF-κB; GDF9; apoptosis
Zhen YANG , Shaoru JIANG , Xiaoyan CHEN , Xiaolin CHEN , Weimin DENG , Xinyu. GUO . Effect of Jinghou Zengzhi Granules on ovarian GDF9 secretion and granulosa cells apoptosis in controlled ovarian hyperstimulation rats[J]. The Journal of Practical Medicine, 2024 , 40(7) : 918 -923 . DOI: 10.3969/j.issn.1006-5725.2024.07.007
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