Objective The study aimed to compare the diagnostic efficacy of QuantiFERON?TB Gold （QFT?TB） detection of specific cellular immune IGRAs in tuberculosis diagnostic laboratory for pulmonary tuberculosis， extrapulmonary tuberculosis and special population samples in vitro， which may provide evidence for clinical diagnosis and treatment. Methods A total of 546 patients with tuberculosis （AFB + 146 cases， AFB?247 cases）， 117 patients with molecular biology positive tuberculosis （Xpert 69 cases， TB?DNA 48 cases） and 36 patients with histopathological positive were collected from January to July 2023. There were 72 cases of extrapulmonary tuberculosis， 276 cases of pleural effusion and 25 cases of ascites. QFT?TB method was used for detection， chi?square test was applied for comparison between groups， and the methodological evaluation of positive rate and coincidence rate were all compared. Results The positive rates of QFT?TB in pulmonary tuberculosis， extrapulmonary tuberculosis and close contacts were 83.69 %， 69.44 %， and 32.41 %， respectively. The coincidence rates of QFT?TB in AFB +， GeneXpert， TB?DNA and pathological confirmed tuberculosis patients were 91.09%， 88.40%， 81.25%， and 72.22%， respectively. The positive rate of pleural effusion in patients with tuberculous pleurisy was 60.50 %， and the uncertainty rate was 29.71%. The positive rate of ascites was 44.00 % and the uncertainty was 36.00%. Conclusion QFT?TB test has good value in the auxiliary diagnosis of pulmonary tuberculosis， and has certain reference significance for the diagnosis of extrapulmonary tuberculosis based on the detection of pleural fluids and ascites.