The Journal of Practical Medicine >
LAMP3 inhibited the proliferation， metastatic and PC⁃3⁃induced vasculogenesis of HUVEC by regulating VEGF/AKT signaling
Received date: 2023-05-11
Online published: 2024-03-06
Objective To explore the impactof Lysosome?Associated Membrane Protein 3 （LAMP3） on theproliferation， migration and angiogenesis of PC?3 cells. Methods LAMP3 expression in normal prostate epithelial cells and prostate cancer bone metastasis cells was detected using western blot and RT?PCR. Stable LAMP3?silenced PC?3 cells were constructed， and the effects of LAMP3 on proliferation， invasion， and migration of PC?3 cells were assessed using CCK8， scratch assay， and transwell assay， respectively. ELISA and angiogenesis assays were employed to examine the expression of VEGF and MMP9， as well as angiogenesis of HUVEC cells induced by PC?3 cells. Finally， WB and RT?PCR were used to detect the expression of VEGF， AKT/p?AKT. Results Our findings showed that the expression level of LAMP3 was significantly higherin prostate cellsthan in normal prostate epithelial cells， especially in PC?3 cells （P < 0.05）. We also found that silencing LAMP3 could inhibit the proliferation， migration and invasion of PC?3 cells， along with the expression of VEGF and MMP9 and the PC?3 cells?induced angiogenesis， and these results were statistically significant （P < 0.05）. Furthermore， LAMP3 downregulated the expression of VEGF and AKT/p?AKT in PC?3 cells. Conclusion LAMP3 can affect the proliferation， migrationand angiogenesis of PC?3 cells through the regulation of VEGF/AKT pathway. Thus， LAMP3 might be a potential therapeutic target for prostate cancer bone metastasis.
Canwei CHEN , Zhuangwen LIAO , Ziwen FAN , Shuai HUANG , Yan HUANG , Binwei CHEN . LAMP3 inhibited the proliferation， metastatic and PC⁃3⁃induced vasculogenesis of HUVEC by regulating VEGF/AKT signaling[J]. The Journal of Practical Medicine, 2024 , 40(2) : 182 -187 . DOI: 10.3969/j.issn.1006-5725.2024.02.010
| 1 | SAWADA Y ， KIKUGAWA T ， IIO H， et al . GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer［J］. Int J Cancer， 2020，146（5）：1369-1382. doi:10.1002/ijc.32554 |
| 2 | LIANG Y ， ZHANG X ， MA C ， et al . m（6）A Methylation Regulators Are Predictive Biomarkers for Tumour Metastasis in Prostate Cancer［J］. Cancers （Basel）， 2022，14（16）：4035. doi:10.3390/cancers14164035 |
| 3 | 吴俊檄， 唐欲博， 令狐熙涛， 等 . 肉桂醛对PC-3细胞增殖、EMT及干细胞特性的影响［J］. 实用医学杂志， 2023，39（3）： 298-302， 308. doi:10.3969/j.issn.1006-5725.2023.03.006 |
| 4 | LI Y ， DU W ， HAN J ， et al . LAMP3 promotes the invasion of osteosarcoma cells via SPP1 signaling［J］. Mol Med Rep， 2017，16（5）：5947-5953. doi:10.3892/mmr.2017.7349 |
| 5 | YANG C ， SHEN S ， ZHENG X ， et al . Long noncoding RNA HAGLR acts as a microRNA-143-5p sponge to regulate epithelial-mesenchymal transition and metastatic potential in esophageal cancer by regulating LAMP3［J］. FASEB J， 2019，33（9）：10490-10504. doi:10.1096/fj.201802543rr |
| 6 | WANG D ， CAO X ， ZHANG Y ， et al . LAMP3 expression correlated with poor clinical outcome in human ovarian cancer［J］. Tumour Biol， 2017，39（3）：1010428317695014. doi:10.1177/1010428317695014 |
| 7 | GONZALEZ S ， GUPTA J ， VILLA E ， et al . Replication of genome-wide association study （GWAS） susceptibility loci in a Latino bipolar disorder cohort［J］. Bipolar Disord， 2016，18（6）： 520-527. doi:10.1111/bdi.12438 |
| 8 | 谢尚延， 黄帅， 王斌， 等 . 桦木酸在缺氧条件下对PC-3细胞干性、侵袭、迁移、黏附及HIF-1α表达的影响［J］. 实用医学杂志， 2021，37（24）：3119-3125. |
| 9 | HE P ， XIONG G ， GUO W ， et al . Long non-coding RNA CCAT2 promotes prostate cancer cell proliferation and invasion by regulating the Wnt/beta-catenin signaling pathway［J］. Oncol Lett， 2020， 20（4）：97. doi:10.3892/ol.2020.11958 |
| 10 | BRADY O A ， MARTINA J A ， PUERTOLLANO R . Emerging roles for TFEB in the immune response and inflammation［J］. Autophagy， 2018，14（2）：181-189. doi:10.1080/15548627.2017.1313943 |
| 11 | TANAKA T ， WARNER B M ， ODANI T ， et al . LAMP3 induces apoptosis and autoantigen release in Sjogren's syndrome patients［J］. Sci Rep， 2020， 10（1）：15169. doi:10.1038/s41598-020-71669-5 |
| 12 | TANAKA T ， WARNER B M ， MICHAEL D G ， et al . LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization［J］. Autophagy， 2022，18（7）：1629-1647. doi:10.1080/15548627.2021.1995150 |
| 13 | NAGELKERKE A ， SIEUWERTS A M ， BUSSINK J ， et al . LAMP3 is involved in tamoxifen resistance in breast cancer cells through the modulation of autophagy［J］. Endocr Relat Cancer， 2014，21（1）：101-112. doi:10.1530/erc-13-0183 |
| 14 | BURTON T D ， FEDELE A O ， XIE J ， et al . The gene for the lysosomal protein LAMP3 is a direct target of the transcription factor ATF4［J］. J Biol Chem， 2020，295（21）：7418-7430. doi:10.1074/jbc.ra119.011864 |
| 15 | WU H ， LI J ， CHEN J ， et al . Efficacy of radiation exposure in laryngeal squamous cell carcinoma is mediated by the LAMP3/LAMC2/tenascin-C pathway［J］. Exp Biol Med （Maywood）， 2019，244（13）：1070-1080. doi:10.1177/1535370219867643 |
| 16 | LIU S ， YUE J ， DU W ， et al . LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53［J］. Cell Mol Biol Lett， 2018， 23：33. doi:10.1186/s11658-018-0099-8 |
| 17 | VIALLARD C ， LARRIVEE B . Tumor angiogenesis and vascular normalization： alternative therapeutic targets［J］. Angiogenesis， 2017，20（4）：409-426. doi:10.1007/s10456-017-9562-9 |
| 18 | CAO J ， LIU X ， YANG Y ， et al . Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/ BAI1 signaling pathway［J］. Angiogenesis， 2020，23（3）：325-338. doi:10.1007/s10456-020-09707-z |
| 19 | UNTERLEUTHNER D ， NEUHOLD P ， SCHWARZ K ， et al . Cancer-associated fibroblast-derived WNT2 increases tumor angiogenesis in colon cancer［J］. Angiogenesis， 2020，23（2）：159-177. doi:10.1007/s10456-019-09688-8 |
| 20 | ABATE M ， PISANTI S ， CAPUTO M ， et al . 3-Hydroxytyrosol Promotes Angiogenesis In Vitro by Stimulating Endothelial Cell Migration［J］. Int J Mol Sci， 2020，21（10）：3657. doi:10.3390/ijms21103657 |
| 21 | JIA Z ， HUANG Y ， JI X ， et al . Ticagrelor and clopidogrel suppress NF-kappaB signaling pathway to alleviate LPS-induced dysfunction in vein endothelial cells［J］. BMC Cardiovasc Disord， 2019，19（1）：318. doi:10.1186/s12872-019-01287-1 |
| 22 | IOANNIDOU E ， MOSCHETTA M ， SHAH S ， et al . Angiogenesis and Anti-Angiogenic Treatment in Prostate Cancer： Mechanisms of Action and Molecular Targets［J］. Int J Mol Sci， 2021，22（18）：9926. doi:10.3390/ijms22189926 |
| 23 | LIU S Y ， ZHAO Z Y ， QIAO Z ， et al . LncRNA PCAT1 Interacts with DKC1 to Regulate Proliferation， Invasion and Apoptosis in NSCLC Cells via the VEGF/AKT/Bcl2/Caspase9 Pathway［J］. Cell Transplant， 2021，30：963689720986071. doi:10.1177/0963689720986071 |
| 24 | YANG L Q ， YU S P ， YANG Y T ， et al . Muscone derivative ZM-32 inhibits breast tumor angiogenesis by suppressing HuR-mediated VEGF and MMP9 expression［J］. Biomed Pharmacother， 2021，136：111265. doi:10.1016/j.biopha.2021.111265 |
| 25 | ZENG Y ， WEI L ， LALI M S ， et al . miR-150-5p mediates extravillous trophoblast cell migration and angiogenesis functions by regulating VEGF and MMP9［J］. Placenta， 2020，93：94-100. doi:10.1016/j.placenta.2020.02.019 |
| 26 | DUAN M X ， ZHOU H ， WU Q Q ， et al . Andrographolide Protects against HG-Induced Inflammation， Apoptosis， Migration， and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs［J］. Mediators Inflamm， 2019，2019：6168340. doi:10.1155/2019/6168340 |
| 27 | YU R ， ZHONG J ， ZHOU Q ， et al . Kaempferol prevents angiogenesis of rat intestinal microvascular endothelial cells induced by LPS and TNF-alpha via inhibiting VEGF/Akt/p38 signaling pathways and maintaining gut-vascular barrier integrity［J］. Chem Biol Interact， 2022，366：110135. doi:10.1016/j.cbi.2022.110135 |
/
| 〈 |
|
〉 |