The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 1: 91 - 96

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.01.016

Clinical Research

Study on the mutation characteristics of streptomycin genes of Multidrug-Resistant Mycobacterium Tuberculosis in Jiangxi Province

  • Shengming YU ,
  • Lianghua XIA ,
  • Jiahuan ZHAN ,
  • Siqi LIU ,
  • Wei WANG ,
  • Liang YAN ,
  • Kaisen. CHEN
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  • *.Gaoxin Branch of the First Affiliated Hospital of Nanchang University，Nanchang 330096，China
    *.School of Public Health，Nanchang University，Nanchang 330019，China

Received date: 2023-08-01

  Online published: 2024-01-24

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Abstract

Objective To understand the molecular characteristics of Streptomycin （SM） resistance in multidrug-resistanttuberculosis（MDR-TB） in Jiangxi Province， and to explore the relationship between SM resistant genes （rpsL， rrs and gidB） mutations and SM resistant phenotypes in Beijing genotype TB. Methods 106 non-replicated MDR-TB isolates were collected from Gaoxin Branch of The First Affiliated Hospital of Nanchang University and Jiangxi Provincial Chest Hospital from January to December 2021， and tested for drug-resistance phenotypes， whether they were Beijing genotype or not and the characteristics of rpsL， rrs and gidB gene mutations. Chi-square test was performed to determine whether rpsL， rrs and gidB mutations were related to genotypes and drug-resistance phenotypes. Results Among 106 cases of MDR-TB， 76 cases were resistant to SM. A total of 58 cases had rpsL 43A > G mutation， 8 cases had 88A > G mutation， 5 cases had rrs mutation， and 3 cases had gidB mutation. Statistical analysis showed that the coincidence rate of gene mutation and phenotypic drug-resistance detection was 89.6%， and the specificity and sensitivity were 86.7% and 90.8%， respectively. The isolated rate of Beijing genotype TB was 88.7%， and the drug-resistant gene mutations were mainly concentrated in rpsL and rrs， while the drug-resistant mutations of non-Beijing genotype were mainly concentrated in gidB； in addition， Beijing genotype bacteria were more prone to gene mutations （P = 0.013）， but there was no difference in phenotypic drug-resistance. Conclusions Mutations in rpsL， rrs， and gidB genes have a good coincidence rate with phenotypic drug-resistance， and molecular biology can be used to detect directly drug-resistance genes to predict bacterial resistance； TB genotypes are strongly associated with streptomycin resistance characteristics.

Key words： multidrug-resistant tuberculosis; streptomycin resistance; beijing genotype; Jiangxi province

Cite this article

Shengming YU , Lianghua XIA , Jiahuan ZHAN , Siqi LIU , Wei WANG , Liang YAN , Kaisen. CHEN . Study on the mutation characteristics of streptomycin genes of Multidrug-Resistant Mycobacterium Tuberculosis in Jiangxi Province[J]. The Journal of Practical Medicine, 2024 , 40(1) : 91 -96 . DOI: 10.3969/j.issn.1006-5725.2024.01.016

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