The Journal of Practical Medicine >

2024 , Vol. 40 >Issue 1: 7 - 12

DOI: https://doi.org/10.3969/j.issn.1006-5725.2024.01.002

Feature Reports：Lung cancer

RNA sequencing⁃based research on the immune regulation mechanism of C⁃MET in lung cancer

  • Yue XU ,
  • Yanbin ZHANG ,
  • Shan. SU
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  • *.Qingyuan People′s Hospital，Qingyuan 511436，China

Received date: 2023-06-23

  Online published: 2024-01-24

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Abstract

Objective To analyze the immune regulation mechanism of C-MET expression in non-small cell lung cancer by transcriptome sequencing technology. Methods The C-MET expression of lung adenocarcinoma cell line （H1993） and lung squamous cell carcinoma cell line （EBC-1） with high C-MET expression was silenced using siRNA molecular interference technology. The differentially expressed genes （DEGs） before and after C-MET silencing were detected using transcriptome sequencing technology. The signal pathways and related genes of the immune microenvironment in which C-MET may participate in regulation were excavated through bioinformatics analysis. Finally， the co-culture technique of human immune cells with H1993 and EBC-1 was used to verify the effect of C-MET on immune factors such as INF-γ， INF-β and CXCL-10. Results We detected 505 DEGs in total using transcriptome sequencing.There were 38 differentially expressed genes in the C-MET regulation group before and after H1993， 24 upregulated differentially expressed genes， and 14 downregulated differentially expressed genes， respectively. There are a total of 467 differentially expressed genes in the C-MET regulation group of EBC-1， 347 upregulated differentially expressed genes， and 121 downregulated differentially expressed genes， respectively. KEGG analysis of differential genes suggested that C-MET expression might participate in the regulation of immune cell regulatory factors through the IL-17 signaling pathway， white blood cell differentiation， cytokine receptor activity， cell cycle， cytokine receptor activity， and cytokine-cytokine receptor interaction. The effect of C-MET on immune factor secretion was verified using the co-culture technique of lung cancer cells and human immune cells， and the results of Rt-qPCR assay suggested，the mRNA transcriptional level of INF-γ in PBMC co-cultured with the C-MET high expression group was 77 times that of the low expression group， and the mRNA transcriptional level of CXCL-10 was 1.6 times that of the low expression group. The mRNA transcriptional level of INF--β was twice as high as that of the low expression group. Conclusion C-MET expression may participate in the regulation of tumor surrounding immune microenvironment through IL-17 signaling pathway， leukocyte differentiation， and cytokine receptor activity pathway.

Key words： non-small cell lung cancer; C-MET; immune microenvironment; expression profile sequencing

Cite this article

Yue XU , Yanbin ZHANG , Shan. SU . RNA sequencing⁃based research on the immune regulation mechanism of C⁃MET in lung cancer[J]. The Journal of Practical Medicine, 2024 , 40(1) : 7 -12 . DOI: 10.3969/j.issn.1006-5725.2024.01.002

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