The Journal of Practical Medicine >

2023 , Vol. 39 >Issue 24: 3182 - 3187

DOI: https://doi.org/10.3969/j.issn.1006-5725.2023.24.006

Basic Research

Impacts of tanshinone IIA on lipopolysaccharide induced proliferation and apoptosis of dental pulp stem cells by regulating the Fas/FasL signaling pathway

  • Wanchao WU ,
  • Yuhuan HAN ,
  • Lijie. LI
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  • *.Department of Stomatology，Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010050，China

Received date: 2023-05-17

  Online published: 2024-01-10

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Abstract

Objective To investigate the impacts of tanshinone ⅡA （Tan ⅡA） on lipopolysaccharide （LPS） induced proliferation and apoptosis of dental pulp stem cells by regulating the fatty acid synthase （Fas）/fatty acid synthase ligand （FasL） signaling pathway. Methods Identification of human pulp stem cells （hDPSCs） isolated from the third molar of 18 ~ 20 years old patients requiring orthodontics. Lps-induced hDPSCs were treated with low， medium and high doses of Tan ⅡA， and then human recombinant FasL protein （rh FasL） was used to intervene the LPS-induced hDPSCs after high dose Tan ⅡA. Proliferation and apoptosis of hDPSCs， levels of tumor necrosis factor-α （TNF-α） and interleukin （IL）-6 in hDPSCs supernatant， proliferating cell nuclear antigen （PCNA）， Cleaved aspartate-specific cysteine proteinase-3 （Cleaved Caspase-3） ， Fas， FasL protein expression were detected. Results hDPSCs were successfully isolated. In a dose-dependent manner， Tan IIA promoted LPS-induced proliferation， inhibited apoptosis， up-regulated PCNA protein expression， and inhibited TNF-α， IL-6 level， Cleaved Caspase-3， Fas， and FasL protein expression. The effect of rh FasL on LPS-induced dental pulp stem cells was opposite to the above indexes （P < 0.05）. rh FasL attenuates the effect of high-dose Tan ⅡA on proliferation and apoptosis of LPS-induced dental pulp stem cells. Conclusion Tan ⅡA may promote LPS induced hDPSCs proliferation and inhibit apoptosis by inhibiting the Fas/FasL signaling pathway.

Key words： tanshinone ⅡA; fatty acid synthase/fatty acid synthase ligand signaling pathway; dental pulp stem cells; proliferation; apoptosis

Cite this article

Wanchao WU , Yuhuan HAN , Lijie. LI . Impacts of tanshinone IIA on lipopolysaccharide induced proliferation and apoptosis of dental pulp stem cells by regulating the Fas/FasL signaling pathway[J]. The Journal of Practical Medicine, 2023 , 39(24) : 3182 -3187 . DOI: 10.3969/j.issn.1006-5725.2023.24.006

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